Strain identifier

BacDive ID: 3113

Type strain: Yes

Species: Corynebacterium pseudotuberculosis

Strain Designation: E23

Strain history: CIP <- 1987, NCTC <- 1931, G.F. Petrie: strain E23 <- J.B. Buxton, Corynebacterium ovis

NCBI tax ID(s): 1719 (species)

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General

@ref: 9010

BacDive-ID: 3113

DSM-Number: 20689

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Corynebacterium pseudotuberculosis E23 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from infected gland of sheep.

NCBI tax id

  • NCBI tax id: 1719
  • Matching level: species

strain history

@refhistory
9010<- NCTC <- G.F. Petrie <- J.B. Buxton (Corynebacterium ovis)
67770NCTC 3450 <-- G. F. Petrie <-- J. B. Buxton E23.
122002CIP <- 1987, NCTC <- 1931, G.F. Petrie: strain E23 <- J.B. Buxton, Corynebacterium ovis

doi: 10.13145/bacdive3113.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium pseudotuberculosis
  • full scientific name: Corynebacterium pseudotuberculosis (Buchanan 1911) Eberson 1918 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus pseudotuberculosis

@ref: 9010

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium pseudotuberculosis

full scientific name: Corynebacterium pseudotuberculosis (Buchanan 1911) Eberson 1918 emend. Nouioui et al. 2018

strain designation: E23

type strain: yes

Morphology

cell morphology

  • @ref: 122002
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refincubation periodmedium used
1877010-14 daysISP 2
1877010-14 daysISP 3
1877010-14 daysISP 4
1877010-14 daysISP 5
1877010-14 daysISP 6
1877010-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18770noISP 2
18770noISP 3
18770noISP 4
18770noISP 5
18770noISP 6
18770noISP 7

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18770ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18770ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18770ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18770ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18770ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18770ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9010TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yesName: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/92
9010COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693
38112MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
122002CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
122002CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
18770positiveoptimum37mesophilic
9010positivegrowth37mesophilic
38112positivegrowth37mesophilic
44589positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 44589
  • oxygen tolerance: microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837917632nitrate-reduction
683794853esculin-hydrolysis
683795291gelatin-hydrolysis
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379catalase+1.11.1.6
68379gelatinase-
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    44589C16:022.416
    44589C18:018.718
    44589C17:1 ISO I/C16:0 DMA2.416.481
    44589C18:1 ω9c19.617.769
    44589C18:2 ω6,9c/C18:0 ANTE36.917.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
18770---+---------------
9010---+--+--+-++--+/-+++-+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18770+++------+----+----

Isolation, sampling and environmental information

isolation

@refsample typecountrycontinent
9010infected gland of sheep
44589Sheep gland infectionSouth AmericaMiddle and South America
67770Infected gland of sheepSouth America
122002Animal, Sheep, infected glandSouth AmericaMiddle and South America

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Mammals#Caprinae (Sheep/Goat)
#Host Body-Site

taxonmaps

  • @ref: 69479
  • File name: preview.99_248.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_101;97_108;98_117;99_248&stattab=map
  • Last taxonomy: Corynebacterium
  • 16S sequence: X81916
  • Sequence Identity:
  • Total samples: 30067
  • soil counts: 2353
  • aquatic counts: 2458
  • animal counts: 24362
  • plant counts: 894

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
187702Risk group (German classification)
90102Risk group (German classification)
1220022Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Corynebacterium pseudotuberculosis strain ATCC 19410 16S ribosomal RNA gene, partial sequenceGQ1183421442ena1719
20218C.pseudotuberculosis 16S rRNA gene (CIP 102968T)X819161397ena1719
20218Corynebacterium pseudotuberculosis strain DSM 20689 16S-23S ribosomal RNA intergenic spacer, complete sequenceAF536509377ena1719
20218Corynebacterium pseudotuberculosis 16S rRNA gene, strain NCTC 3450X842551446ena1719
9010Corynebacterium pseudotuberculosis (strain ATCC 19410) gene for 16S ribosomal RNAD385791472ena1719

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium pseudotuberculosis ATCC 19410GCA_002155265completencbi1719
66792Corynebacterium pseudotuberculosis strain ATCC 194101719.2381completepatric1719
66792Corynebacterium pseudotuberculosis strain DSM 206891719.2642wgspatric1719
66792Corynebacterium pseudotuberculosis DSM 206892756170169draftimg1719
66792Corynebacterium pseudotuberculosis ATCC 194102872045035completeimg1719
67770Corynebacterium pseudotuberculosis DSM 20689GCA_003634885contigncbi1719

GC content

@refGC-contentmethod
6777052.2genome sequence analysis
6777051.8thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno97.594no
gram-positiveyes94.119no
anaerobicno99.013no
halophileyes72.539no
spore-formingno93.439no
glucose-utilyes89.043no
aerobicno83.587yes
thermophileno95.537yes
motileno93.963no
glucose-fermentyes70.128no

External links

@ref: 9010

culture collection no.: DSM 20689, ATCC 19410, NCTC 3450, CCUG 2806, LMG 19054, CIP 102968, JCM 9389, BCRC 14872, CCM 3428, CECT 808, IFO 15363, NBRC 15363, PCM 2052

straininfo link

  • @ref: 72631
  • straininfo: 12980

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19661496Corynebacterium mustelae sp. nov., isolated from a ferret with lethal sepsis.Funke G, Frodl R, Bernard KAInt J Syst Evol Microbiol10.1099/ijs.0.010942-02009Animals, Autopsy/*veterinary, Bacterial Proteins/genetics, Bacterial Typing Techniques, Corynebacterium/*classification/genetics/*isolation & purification, Corynebacterium Infections/microbiology/*veterinary, DNA, Ribosomal/analysis/genetics, Ferrets/*microbiology, Genes, rRNA, Genotype, Lung/microbiology, Male, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sepsis/microbiology/*veterinary, Sequence Analysis, DNA, Species SpecificityGenetics
Enzymology22911589Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR.Dorneles EM, Santana JA, Andrade GI, Santos EL, Guimaraes AS, Mota RA, Santos AS, Miyoshi A, Azevedo V, Gouveia AM, Lage AP, Heinemann MBGenet Mol Res10.4238/2012.August.6.92012Animals, Brazil, Cluster Analysis, Consensus Sequence/*genetics, Corynebacterium pseudotuberculosis/*genetics/*isolation & purification, DNA Fingerprinting, DNA, Intergenic/*genetics, Enterobacteriaceae/genetics, Goats/*microbiology, Polymerase Chain Reaction/*methods, Repetitive Sequences, Nucleic Acid/*geneticsPhylogeny
Phylogeny24901343Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates.Dorneles EM, Santana JA, Ribeiro D, Dorella FA, Guimaraes AS, Moawad MS, Selim SA, Garaldi AL, Miyoshi A, Ribeiro MG, Gouveia AM, Azevedo V, Heinemann MB, Lage APPLoS One10.1371/journal.pone.00987582014Animal Diseases/microbiology, Animals, Cluster Analysis, Corynebacterium Infections/veterinary, Corynebacterium pseudotuberculosis/*classification/*genetics/isolation & purification, *DNA, Intergenic, Genotype, Molecular Typing, Phylogeny, Polymerase Chain Reaction, *Repetitive Sequences, Nucleic AcidGenetics
Phylogeny27979735Molecular epidemiology of Corynebacterium pseudotuberculosis isolated from horses in California.Haas DJ, Dorneles EM, Spier SJ, Carroll SP, Edman J, Azevedo VA, Heinemann MB, Lage APInfect Genet Evol10.1016/j.meegid.2016.12.0112016Animals, Bacterial Typing Techniques, California/epidemiology, Cluster Analysis, Corynebacterium Infections/epidemiology/microbiology/pathology/*veterinary, Corynebacterium pseudotuberculosis/classification/*genetics/isolation & purification, DNA Fingerprinting, DNA, Bacterial/*genetics, DNA, Intergenic/*genetics, Genetic Variation, Genotype, Horse Diseases/*epidemiology/microbiology/pathology, Horses/microbiology, Molecular Epidemiology, Phylogeny, Severity of Illness IndexGenetics
Phylogeny28946887Quadruplex PCR assay for identification of Corynebacterium pseudotuberculosis differentiating biovar Ovis and Equi.Almeida S, Dorneles EMS, Diniz C, Abreu V, Sousa C, Alves J, Carneiro A, Bagano P, Spier S, Barh D, Lage AP, Figueiredo H, Azevedo VBMC Vet Res10.1186/s12917-017-1210-52017Corynebacterium pseudotuberculosis/classification/*genetics/*isolation & purification, DNA, Bacterial/genetics, Genome, Bacterial, Polymerase Chain Reaction/*methods, Species SpecificityGenetics
30173853Caseous Lymphadenitis Caused by Corynebacterium pseudotuberculosis in Alpine Chamois (Rupicapra r. rupicapra): a Review of 98 Cases.Domenis L, Spedicato R, Pepe E, Orusa R, Robetto SJ Comp Pathol10.1016/j.jcpa.2018.04.0032018Animals, Corynebacterium Infections/*veterinary, Corynebacterium pseudotuberculosis, Female, Lymphadenitis/*veterinary, Male, Rupicapra/*microbiology
Pathogenicity30721090Lawsonia inermis L. leaves aqueous extract as a natural antioxidant and antibacterial product.Pasandi Pour A, Farahbakhsh HNat Prod Res10.1080/14786419.2019.15690062019Anti-Bacterial Agents/chemistry/*pharmacology, Antioxidants/administration & dosage/chemistry/*pharmacology, Dose-Response Relationship, Drug, Drug Evaluation, Preclinical, Ecotype, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects, Lawsonia Plant/*chemistry, Microbial Sensitivity Tests, Naphthoquinones/analysis, Plant Extracts/chemistry, Plant Leaves/chemistry
Phylogeny31310200Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp. nov., Corynebacterium pseudopelargi sp. nov., and Corynebacterium gerontici sp. nov.Busse HJ, Kleinhagauer T, Glaeser SP, Spergser J, Kampfer P, Ruckert CInt J Syst Evol Microbiol10.1099/ijsem.0.0035802019Animals, Bacterial Typing Techniques, Base Composition, Birds/*microbiology, Corynebacterium/*classification/isolation & purification, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Genes, Bacterial, Nasal Cavity/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9010Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20689)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20689
18770Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20689.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38112Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14713
44589Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2806)https://www.ccug.se/strain?id=2806
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72631Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12980.1StrainInfo: A central database for resolving microbial strain identifiers
122002Curators of the CIPCollection of Institut Pasteur (CIP 102968)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102968