Strain identifier

BacDive ID: 3105

Type strain: Yes

Species: Corynebacterium minutissimum

Strain Designation: Barton

Strain history: CIP <- 1984, ATCC <- D. Taplin <- I. Sarkany, London, UK

NCBI tax ID(s): 1223512 (strain), 38301 (species)

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General

@ref: 8871

BacDive-ID: 3105

DSM-Number: 20651

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic

description: Corynebacterium minutissimum Barton is an obligate aerobe, mesophilic bacterium that was isolated from erythrasma.

NCBI tax id

NCBI tax idMatching level
1223512strain
38301species

strain history

@refhistory
8871<- NCTC <- I. Sarkany, Barton
67770NCTC 10288 <-- I. Sarkany.
120962CIP <- 1984, ATCC <- D. Taplin <- I. Sarkany, London, UK

doi: 10.13145/bacdive3105.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium minutissimum
  • full scientific name: Corynebacterium minutissimum (ex Sarkany et al. 1962) Collins and Jones 1983

@ref: 8871

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium minutissimum

full scientific name: Corynebacterium minutissimum (ex Sarkany et al. 1962) Collins and Jones 1983 emend. Yassin et al. 2002 emend. Nouioui et al. 2018

strain designation: Barton

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no97.787
69480100positive
120962nopositiverod-shaped

colony morphology

@refcolony colorincubation periodmedium used
18454Light ivory (1015)10-14 daysISP 2
18454Ivory (1014)10-14 daysISP 4
18454Ivory (1014)10-14 daysISP 5
18454Beige (1001)10-14 daysISP 6
18454Ivory (1014)10-14 daysISP 7
120962

multicellular morphology

@refforms multicellular complexmedium name
18454noISP 2
18454noISP 4
18454noISP 5
18454noISP 6
18454noISP 7

multimedia

@refmultimedia contentintellectual property rightscaption
8871https://www.dsmz.de/microorganisms/photos/DSM_20651-1.jpg© Leibniz-Institut DSMZ
8871https://www.dsmz.de/microorganisms/photos/DSM_20651.jpg© Leibniz-Institut DSMZMedium 693 37°C

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18454ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18454ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18454ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18454ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18454ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
8871COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693
8871TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yesName: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535
38086MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120962CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
18454positiveoptimum37mesophilic
8871positivegrowth37mesophilic
38086positivegrowth37mesophilic
67770positivegrowth37mesophilic
120962positivegrowth25-41
120962nogrowth10psychrophilic
120962nogrowth15psychrophilic
120962nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120962
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no98
69480no99.996

halophily

@refsaltgrowthtested relationconcentration
18454NaClpositivemaximum10 %
120962NaClpositivegrowth0-8 %
120962NaClnogrowth10 %

murein

  • @ref: 8871
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-8(H2), MK-9(H2), MK-8(H2)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68368L-arabinose-fermentation30849
68368amygdalin-fermentation27613
68368melibiose-fermentation28053
68368sucrose+fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol-fermentation16899
68368D-glucose+fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016
120962citrate-carbon source16947
120962esculin-hydrolysis4853
120962hippurate-hydrolysis606565
120962nitrate-reduction17632
120962nitrite-reduction16301
120962nitrate-respiration17632
68379esculin-hydrolysis4853
68379gelatin-hydrolysis5291
68379D-ribose-fermentation16988
68379D-xylose-fermentation65327
68379D-mannitol-fermentation16899
68379lactose-fermentation17716
68379glycogen-fermentation28087

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
12096235581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
12096215688acetoin-
12096217234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68382alkaline phosphatase+3.1.3.1
68379catalase+1.11.1.6
68379gelatinase-
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
120962oxidase-
120962beta-galactosidase-3.2.1.23
120962alcohol dehydrogenase-1.1.1.1
120962gelatinase-
120962amylase+
120962DNase-
120962caseinase-3.4.21.50
120962catalase+1.11.1.6
120962tween esterase-
120962gamma-glutamyltransferase-2.3.2.2
120962lecithinase-
120962lipase-
120962lysine decarboxylase-4.1.1.18
120962ornithine decarboxylase-4.1.1.17
120962protease-
120962tryptophan deaminase-
120962urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
18454---+-----+---------
8871+----------+---+-+--+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18454+-+-+-++-+---------
120962+++++--+-++---------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARA
18454---------+-+----+---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120962+---------+++--------+-----+--+------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120962++-----+-++----++-+------------+-----------------+---------+-+-----------+-+--+++--------+++--+-++-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
8871erythrasma
44224Human trunk of female,erythrasmaLondonUnited KingdomGBREurope
67770Erythrasma
120962Human, ErythrasmaLondonUnited KingdomGBREurope1962

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host Body-Site#Organ#Skin, Nail, Hair

taxonmaps

  • @ref: 69479
  • File name: preview.99_3289.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_322;97_835;98_971;99_3289&stattab=map
  • Last taxonomy: Corynebacterium
  • 16S sequence: X84679
  • Sequence Identity:
  • Total samples: 22059
  • soil counts: 1440
  • aquatic counts: 1218
  • animal counts: 18979
  • plant counts: 422

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
184542German classification
88712Risk group (German classification)
1209622Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218C.minutissimum 16S ribosomal DNAX820641400ena38301
8871Corynebacterium minutissimum 16S rRNA gene, strain NCTC 10288, clone 1X846781435ena38301
8871Corynebacterium minutissimum 16S rRNA gene, strain NCTC 10288, clone 2X846791435ena38301

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium minutissimum NCTC10288GCA_900478045completencbi38301
66792Corynebacterium minutissimum ATCC 2334838301.3wgspatric38301
66792Corynebacterium minutissimum NBRC 153611223512.3wgspatric1223512
66792Corynebacterium minutissimum strain FDAARGOS_89438301.37completepatric38301
66792Corynebacterium minutissimum strain NCTC1028838301.16completepatric38301
66792Corynebacterium minutissimum NCTC 102882811995197completeimg38301
66792Corynebacterium minutissimum ATCC 233482645728081draftimg38301
66792Corynebacterium minutissimum NBRC 153612731957584draftimg1223512
67770Corynebacterium minutissimum NBRC 15361GCA_001552395contigncbi1223512
67770Corynebacterium minutissimum ATCC 23348GCA_000805675contigncbi38301

GC content

@refGC-contentmethod
887158.0
6777060genome sequence analysis
6777061high performance liquid chromatography (HPLC)
6777058.9thermal denaturation, midpoint method (Tm)
6777058thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno98no
flagellatedno97.25no
gram-positiveyes95.855no
anaerobicno99.389no
aerobicno60.284no
halophileyes80.545no
spore-formingno94.615no
thermophileno97.76yes
glucose-utilyes86.125no
motileno94.569no
glucose-fermentyes70.907yes

External links

@ref: 8871

culture collection no.: DSM 20651, ATCC 23348, NCTC 10288, CCUG 541, JCM 9387, BCRC 14870, CCM 4728, CECT 4158, CIP 100652, IFO 15361, NBRC 15361

straininfo link

  • @ref: 72623
  • straininfo: 45702

literature

  • Pubmed-ID: 35997235
  • title: The effect of flavonols in Anacardium occidentale L. leaf extracts on skin pathogenic microorganisms.
  • authors: Hashim NS, Tan ML, Ooi KL, Sulaiman SF
  • journal: Nat Prod Res
  • DOI: 10.1080/14786419.2022.2112038
  • year: 2022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8871Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20651)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20651
18454Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20651.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38086Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/12140
44224Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 541)https://www.ccug.se/strain?id=541
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72623Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45702.1StrainInfo: A central database for resolving microbial strain identifiers
120962Curators of the CIPCollection of Institut Pasteur (CIP 100652)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20100652