Strain identifier

BacDive ID: 3102

Type strain: Yes

Species: Corynebacterium matruchotii

Strain Designation: 47

Strain history: CIP <- 1981, NCTC <- 1961, M.N. Gilmour, New-York, USA: strain 47, Bacterionema matruchotii

NCBI tax ID(s): 553207 (strain), 43768 (species)

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General

@ref: 8981

BacDive-ID: 3102

DSM-Number: 20635

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic

description: Corynebacterium matruchotii 47 is a facultative anaerobe, mesophilic bacterium that was isolated from oral calculus.

NCBI tax id

NCBI tax idMatching level
553207strain
43768species

strain history

@refhistory
8981<- NCTC; NCTC 10254 <- ATCC; ATCC 14266 <- M. Gilmour; 47
67770NCTC 10254 <-- M. N. Gilmour.
120859CIP <- 1981, NCTC <- 1961, M.N. Gilmour, New-York, USA: strain 47, Bacterionema matruchotii

doi: 10.13145/bacdive3102.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium matruchotii
  • full scientific name: Corynebacterium matruchotii (Mendel 1919) Collins 1983

  • synonyms

    @refsynonym
    20215Bacterionema matruchotii
    20215Cladothrix matruchoti

@ref: 8981

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium matruchotii

full scientific name: Corynebacterium matruchotii (Mendel 1919) Collins 1983 emend. Nouioui et al. 2018

strain designation: 47

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.24
69480100positive
120859nopositiverod-shaped

colony morphology

@refcolony colorincubation periodmedium used
18451Sand yellow (1002)10-14 daysISP 2
18451Sand yellow (1002)10-14 daysISP 3
18451Beige (1001)10-14 daysISP 4
18451Beige (1001)10-14 daysISP 5
18451Beige (1001)10-14 daysISP 6
18451Beige (1001)10-14 daysISP 7
120859

multicellular morphology

@refforms multicellular complexmedium name
18451noISP 2
18451noISP 3
18451noISP 4
18451noISP 5
18451noISP 6
18451noISP 7

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18451ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18451ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18451ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18451ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18451ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18451ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
8981TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yesName: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/92
34649MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
8981COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf
120859CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
18451positiveoptimum37mesophilic
8981positivegrowth37mesophilic
34649positivegrowth37mesophilic
67770positivegrowth37mesophilic
120859positivegrowth25-41
120859nogrowth10psychrophilic
120859nogrowth15psychrophilic
120859nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120859
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69481no99
69480no99.985

halophily

  • @ref: 120859
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

murein

  • @ref: 8981
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-8(H2), MK-9(H2)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
120859citrate-carbon source16947
120859esculin-hydrolysis4853
120859hippurate+hydrolysis606565
120859nitrate+reduction17632
120859nitrite-reduction16301
120859pyrazinamide+hydrolysis45285
120859nitrate+respiration17632
68379nitrate+reduction17632
68379esculin-hydrolysis4853
68379urea-hydrolysis16199
68379gelatin-hydrolysis5291
68379D-xylose-fermentation65327
68379D-mannitol-fermentation16899
68379lactose-fermentation17716
68379glycogen-fermentation28087

antibiotic resistance

  • @ref: 120859
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 120859
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12085915688acetoin+
12085917234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382trypsin+3.4.21.4
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68379catalase+1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrazinamidase+3.5.1.B15
120859oxidase-
120859beta-galactosidase-3.2.1.23
120859alcohol dehydrogenase+1.1.1.1
120859gelatinase-
120859amylase+
120859DNase+
120859caseinase-3.4.21.50
120859catalase+1.11.1.6
120859tween esterase-
120859gamma-glutamyltransferase-2.3.2.2
120859lecithinase+
120859lipase-
120859lysine decarboxylase-4.1.1.18
120859ornithine decarboxylase-4.1.1.17
120859tryptophan deaminase-
120859urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
18451+++---+----++----+-
8981++---------+/-+/---+/--+/---+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18451-++-++++++----++---
120859-++-+--+-+----++----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120859----+-----+++/--------------+/-+--+---------------+/--+/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120859++-++-+++++-------++-----------+---+--------------------------+--+---------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
8981oral calculus
67770Oral calculus
120859Oral calculusUnited States of AmericaUSANorth America1961

isolation source categories

  • Cat1: #Host Body-Site
  • Cat2: #Oral cavity and airways
  • Cat3: #Tooth

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
184512German classification
89812Risk group (German classification)
1208592Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218C.matruchotii 16S ribosomal DNAX820651344ena43768
20218Corynebacterium matruchotii partial 16S rRNA gene, strain DSM 20635X844431367ena43768

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium matruchotii ATCC 14266GCA_011612265completencbi43768
66792Corynebacterium matruchotii ATCC 14266553207.3wgspatric553207
66792Corynebacterium matruchotii strain ATCC 1426643768.438completepatric43768
66792Corynebacterium matruchotii strain NCTC1025443768.6wgspatric43768
66792Corynebacterium matruchotii ATCC 14266648276634draftimg553207
66792Corynebacterium matruchotii NCTC 102542808606759draftimg43768
67770Corynebacterium matruchotii ATCC 14266GCA_000175375contigncbi553207
67770Corynebacterium matruchotii NCTC10254GCA_900447555contigncbi43768

GC content

@refGC-contentmethod
898155
6777056thermal denaturation, midpoint method (Tm)
6777057.1genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileno96.31no
motileno96.31no
flagellatedno98.34no
flagellatedno98.34no
gram-positiveyes91.984no
gram-positiveyes91.984no
anaerobicno97.383no
anaerobicno97.383no
aerobicno81.913no
aerobicno81.913no
halophileno56.641no
halophileno56.641no
spore-formingno88.284no
spore-formingno88.284no
glucose-utilyes87.336no
glucose-utilyes87.336no
thermophileno84.176no
thermophileno84.176no
glucose-fermentyes62.333no
glucose-fermentyes62.333no

External links

@ref: 8981

culture collection no.: DSM 20635, ATCC 14266, DSM 43761, NCTC 10254, JCM 9386, BCRC 14869, CCUG 27545, CCUG 46620, CIP 81.82, IFO 15360, LMG 19647, NBRC 15360

straininfo link

  • @ref: 72620
  • straininfo: 14129

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology3526757Isolation and characterization of a peptidase from an oral strain of Corynebacterium matruchotii.Fujimura S, Nakamura T, Pulverer GZentralbl Bakteriol Mikrobiol Hyg A10.1016/s0176-6724(86)80028-11986Bacteriocins/metabolism, Corynebacterium/*enzymology, Electrophoresis, Polyacrylamide Gel, Hot Temperature, Hydrogen-Ion Concentration, Peptide Hydrolases/*isolation & purification, Protease Inhibitors/pharmacology, Substrate SpecificityPhylogeny
Phylogeny11230408Diversity within reference strains of Corynebacterium matruchotii includes Corynebacterium durum and a novel organism.Barrett SL, Cookson BT, Carlson LC, Bernard KA, Coyle MBJ Clin Microbiol10.1128/JCM.39.3.943-948.20012001Bacterial Typing Techniques, Base Sequence, Corynebacterium/chemistry/*classification/*genetics/metabolism, Corynebacterium Infections/*microbiology, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis/genetics, Dental Plaque/microbiology, Fatty Acids/analysis, *Genetic Variation, Humans, Molecular Sequence Data, Nucleic Acid Hybridization/methods, Periodontitis/microbiology, RNA, Ribosomal, 16S/genetics, Reference Standards, Sequence Analysis, DNAPathogenicity
Metabolism16403458Transposome mutagenesis of an integral membrane transporter in Corynebacterium matruchotii.Wang C, Hayes B, Vestling MM, Takayama KBiochem Biophys Res Commun10.1016/j.bbrc.2005.12.0972005Biological Transport, Blotting, Southern, Cell Membrane/metabolism, Cell Proliferation, Cell Wall/metabolism, Chromatography, Thin Layer, Cord Factors/chemistry, Corynebacterium/*metabolism, Cytoplasm/metabolism, *DNA Transposable Elements, Lipids/chemistry, Mass Spectrometry, Models, Genetic, *Mutagenesis, Mutation, Mycobacterium tuberculosis/metabolism, Mycolic Acids/chemistry, Polymerase Chain Reaction, RNA/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Time Factors, Transposases/metabolism, Trehalose/chemistry

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8981Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20635)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20635
18451Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20635.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34649Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11248
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72620Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14129.1StrainInfo: A central database for resolving microbial strain identifiers
120859Curators of the CIPCollection of Institut Pasteur (CIP 81.82)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.82