Strain identifier

BacDive ID: 309

Type strain: Yes

Species: Achromobacter ruhlandii

Strain history: CIP <- 1977, M.Y. Popoff, Inst. Pasteur, Paris, France <- DSM <- ATCC <- W. Vishniac, Hydrogenomonas ruhlandii

NCBI tax ID(s): 72557 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 245

BacDive-ID: 309

DSM-Number: 653

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Achromobacter ruhlandii DSM 653 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 72557
  • Matching level: species

strain history

@refhistory
245<- ATCC <- W. Vishniac (Hydrogenomonas ruhlandii)
67770IAM 12600 <-- ATCC 15749 <-- W. Vishniac ("Hydrogenomonas ruhlandii").
122308CIP <- 1977, M.Y. Popoff, Inst. Pasteur, Paris, France <- DSM <- ATCC <- W. Vishniac, Hydrogenomonas ruhlandii

doi: 10.13145/bacdive309.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Achromobacter
  • species: Achromobacter ruhlandii
  • full scientific name: Achromobacter ruhlandii (Packer and Vishniac 1955) Yabuuchi et al. 1998

  • synonyms

    @refsynonym
    20215Hydrogenomonas ruhlandii
    20215Alcaligenes ruhlandii

@ref: 245

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Achromobacter

species: Achromobacter ruhlandii

full scientific name: Achromobacter ruhlandii (Packer and Vishniac 1955) Yabuuchi et al. 1998 emend. Vandamme et al. 2013

type strain: yes

Morphology

cell morphology

  • @ref: 122308
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 245
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36484MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
245NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
245MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81)yesName: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81) Composition: Agar 20.1005 g/l Na2HPO4 x 2 H2O 2.91457 g/l KH2PO4 2.31156 g/l NH4Cl 1.00503 g/l MgSO4 x 7 H2O 0.502512 g/l Ferric ammonium citrate 0.0502513 g/l CaCl2 x 2 H2O 0.0100503 g/l NaVO3 x H2O 0.00502512 g/l Calcium pantothenate 0.00251256 g/l Pyridoxine hydrochloride 0.00251256 g/l Nicotinic acid 0.00251256 g/l Thiamine-HCl x 2 H2O 0.00251256 g/l H3BO3 0.00150754 g/l CoCl2 x 6 H2O 0.00100503 g/l Riboflavin 0.000502513 g/l ZnSO4 x 7 H2O 0.000502513 g/l MnCl2 x 4 H2O 0.000150754 g/l Na2MoO4 x 2 H2O 0.000150754 g/l NiCl2 x 6 H2O 0.000100503 g/l CuCl2 x 2 H2O 5.02513e-05 g/l Vitamin B12 5.02513e-05 g/l Folic acid 1.00503e-05 g/l Biotin 5.02513e-06 g/l Distilled waterhttps://mediadive.dsmz.de/medium/81
122308CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
122308CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
36484positivegrowth30mesophilic
245positivegrowth30mesophilic
67770positivegrowth30mesophilic
122308positivegrowth15-41
122308nogrowth5psychrophilic
122308nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122308
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
122308NaClpositivegrowth0-6 %
122308NaClnogrowth8 %
122308NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12230816947citrate+carbon source
1223084853esculin-hydrolysis
122308606565hippurate-hydrolysis
12230817632nitrate-builds gas from
12230817632nitrate+reduction
12230816301nitrite-builds gas from
12230816301nitrite-reduction
12230815792malonate-assimilation
12230817632nitrate-respiration

antibiotic resistance

  • @ref: 122308
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 122308
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12230815688acetoin-
12230817234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122308oxidase+
122308beta-galactosidase-3.2.1.23
122308alcohol dehydrogenase-1.1.1.1
122308gelatinase-
122308amylase-
122308DNase-
122308caseinase-3.4.21.50
122308catalase+1.11.1.6
122308tween esterase+
122308gamma-glutamyltransferase-2.3.2.2
122308lecithinase-
122308lipase-
122308lysine decarboxylase-4.1.1.18
122308ornithine decarboxylase-4.1.1.17
122308phenylalanine ammonia-lyase-4.3.1.24
122308protease-
122308tryptophan deaminase-
122308urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122308-+++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122308+------------------------------------------+---+++++++----+-++---+---------++++++----+-++++++-+-+++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
245soilUSAUSANorth America
67770Soil
122308Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_954.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7;96_9;97_9;98_570;99_954&stattab=map
  • Last taxonomy: Achromobacter
  • 16S sequence: JQ746426
  • Sequence Identity:
  • Total samples: 7288
  • soil counts: 1429
  • aquatic counts: 1212
  • animal counts: 2896
  • plant counts: 1751

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
2452Risk group (German classification)
1223081Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Achromobacter ruhlandii gene for 16S rRNA, partial sequenceAB0108401318ena72557
245Achromobacter ruhlandii strain CCUG 38886 16S ribosomal RNA gene, partial sequenceJQ7464261309ena72557

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Achromobacter ruhlandii strain LMG 186672557.63wgspatric72557
67770Achromobacter ruhlandii LMG 1866GCA_902859695contigncbi72557

GC content

@refGC-contentmethod
24566.2
6777067.7high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes87.243no
flagellatedno63.691no
gram-positiveno98.592no
anaerobicno98.041no
aerobicyes83.065no
halophileno92.144no
spore-formingno94.001no
thermophileno99.708yes
glucose-utilyes50.934no
glucose-fermentno90.47no

External links

@ref: 245

culture collection no.: DSM 653, ATCC 15749, CCUG 38886, LMG 1866, JCM 20676, CGMCC 1.10394, CIP 77.26, IAM 12600, IAM 12693, IAM 12694, JCM 20751, JCM 20752, NCIMB 11475, VKM B-1333, CCM 7494

straininfo link

  • @ref: 69991
  • straininfo: 389402

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9688077Emendation of genus Achromobacter and Achromobacter xylosoxidans (Yabuuchi and Yano) and proposal of Achromobacter ruhlandii (Packer and Vishniac) comb. nov., Achromobacter piechaudii (Kiredjian et al.) comb. nov., and Achromobacter xylosoxidans subsp. denitrificans (Ruger and Tan) comb. nov.Yabuuchi E, Kawamura Y, Kosako Y, Ezaki TMicrobiol Immunol10.1111/j.1348-0421.1998.tb02306.x1998Alcaligenes/*classification/genetics, Bacterial Typing Techniques, Bordetella/classification, DNA, Bacterial/analysis/chemistry, Humans, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as TopicPathogenicity
Phylogeny24711589Achromobacter sediminum sp. nov., isolated from deep subseafloor sediment of South Pacific Gyre.Zhang Z, Fan X, Gao X, Zhang XHInt J Syst Evol Microbiol10.1099/ijs.0.062265-02014Achromobacter/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Molecular Sequence Data, Pacific Ocean, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNAGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
245Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 653)https://www.dsmz.de/collection/catalogue/details/culture/DSM-653
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36484Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10939
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69991Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389402.1StrainInfo: A central database for resolving microbial strain identifiers
122308Curators of the CIPCollection of Institut Pasteur (CIP 77.26)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2077.26