Strain identifier

BacDive ID: 3085

Type strain: Yes

Species: Corynebacterium flavescens

Strain Designation: OJ8, OJ 8

Strain history: CIP <- 1969, F. Gasser, Inst. Pasteur, Paris, France <- ATCC <- 1919, R.N. Doetsch: strain OJ 8 <- O. Jensen, Microbacterium flavum

NCBI tax ID(s): 28028 (species)

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General

@ref: 8699

BacDive-ID: 3085

DSM-Number: 20296

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Corynebacterium flavescens OJ8 is a mesophilic bacterium that was isolated from cheese.

NCBI tax id

  • NCBI tax id: 28028
  • Matching level: species

strain history

@refhistory
8699<- H.G. Schlegel <- K. Komagata <- ATCC <- R.N. Doetsch, OJ8 <- S. Orla-Jensen (Microbacterium flavum)
67770K. Suzuki CNF 011 <-- AJ 1415 <-- ATCC 10340 <-- R. N. Doetsch OJ 8 <-- S. Orla-Jensen.
119367CIP <- 1969, F. Gasser, Inst. Pasteur, Paris, France <- ATCC <- 1919, R.N. Doetsch: strain OJ 8 <- O. Jensen, Microbacterium flavum

doi: 10.13145/bacdive3085.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium flavescens
  • full scientific name: Corynebacterium flavescens Barksdale et al. 1979 (Approved Lists 1980)

@ref: 8699

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium flavescens

full scientific name: Corynebacterium flavescens Barksdale et al. 1979 emend. Nouioui et al. 2018

strain designation: OJ8, OJ 8

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no95.135
69480100positive

colony morphology

@refcolony colorincubation periodmedium used
18637Deep orange (2011)10-14 daysISP 2
18637Signal yellow (1003)10-14 daysISP 3
18637Ivory (1014)10-14 daysISP 4
18637Signal yellow (1003)10-14 daysISP 5
18637Colorless10-14 daysISP 6

multicellular morphology

@refforms multicellular complexmedium name
18637noISP 2
18637noISP 3
18637noISP 4
18637noISP 5
18637noISP 6

multimedia

  • @ref: 8699
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_20296.jpg
  • caption: Medium 693 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18637ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18637ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18637ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18637ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18637ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
8699TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yesName: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535
33457MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
119367CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
18637positiveoptimum28mesophilic
8699positivegrowth37mesophilic
33457positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.999

halophily

  • @ref: 18637
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 7.5 %

murein

  • @ref: 8699
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-8(H2), MK-9(H2)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016
68379nitrate-reduction17632
68379esculin-hydrolysis4853
68379D-xylose-fermentation65327
68379lactose-fermentation17716
68379sucrose+fermentation17992
68379glycogen-fermentation28087

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379catalase+1.11.1.6
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379pyrrolidonyl arylamidase+3.4.19.3
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    49381C14:0614
    49381C16:035.816
    49381C18:04.418
    49381C18:1 ω9c47.917.769
    49381C18:2 ω6,9c/C18:0 ANTE617.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
18637-++-++---++------+-
49381--++-------++-++-+--+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18637+++-++---++--------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18637---------+-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119367-------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample type
8699cheese
49381Cheese
67770Cheese
119367Food, Cheese

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_5542.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_2678;97_3286;98_4136;99_5542&stattab=map
  • Last taxonomy: Corynebacterium flavescens subclade
  • 16S sequence: X84441
  • Sequence Identity:
  • Total samples: 1799
  • soil counts: 247
  • aquatic counts: 161
  • animal counts: 1362
  • plant counts: 29

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
186371Risk group (German classification)
86991Risk group (German classification)
1193672Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218C.flavescens 16S ribosomal DNAX820601409ena28028
20218Corynebacterium flavescens partial 16S rRNA gene, strain NCDO 1320X844411489ena28028

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium flavescens OJ8GCA_001941465completencbi28028
66792Corynebacterium flavescens strain NBRC 1413628028.6wgspatric28028
66792Corynebacterium flavescens strain OJ828028.4completepatric28028
66792Corynebacterium flavescens DSM 20296 Genome sequencing2775507246completeimg28028
67770Corynebacterium flavescens NBRC 14136GCA_006539465contigncbi28028
67770Corynebacterium flavescens CCUG 28791TGCA_008693105contigncbi28028

GC content

@refGC-contentmethod
869958.3
6777058.3thermal denaturation, midpoint method (Tm)
6777059.9genome sequence analysis
6777056.8thermal denaturation, midpoint method (Tm)
6777058.6thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno97.33no
gram-positiveyes95.29no
anaerobicno98.602no
halophileyes78.705no
spore-formingno93.259no
glucose-utilyes87.522no
aerobicno57.742no
thermophileno98.59yes
motileno91.914no
glucose-fermentyes61.869no

External links

@ref: 8699

culture collection no.: CCUG 28791, DSM 20296, ATCC 10340, NCDO 1320, NCIB 8707, JCM 1317, BCRC 12131, CECT 536, CGMCC 1.1885, CIP 69.5, IAM 12638, IAM 1642, IFO 14136, IMET 11080, IMSNU 21211, KCCM 41095, KCTC 3414, LMG 4046, NBRC 14136, NCCB 42012, NCFB 1320, NCIMB 8707, VKM Ac-1956

straininfo link

  • @ref: 72603
  • straininfo: 13628

literature

  • topic: Pathogenicity
  • Pubmed-ID: 10788790
  • title: Rescuing activity of galactoglycerolipids from cellular lesions induced by 5-aminolevulinic acid.
  • authors: Nakata K
  • journal: J Biochem
  • DOI: 10.1093/oxfordjournals.jbchem.a022674
  • year: 2000
  • mesh: Aminolevulinic Acid/*pharmacology, Brassicaceae/*drug effects, Chlorophyll/biosynthesis, Corynebacterium/*drug effects, Dose-Response Relationship, Drug, Dose-Response Relationship, Radiation, Drug Interactions, Fatty Acids/pharmacology, Free Radical Scavengers/pharmacology, *Galactolipids, *Galactose/analysis/*pharmacology, Glycerides/analysis/*pharmacology, Glycolipids/analysis/*pharmacology, Light, Plant Leaves/chemistry, Plant Stems/drug effects, Porphyrins/biosynthesis, Reactive Oxygen Species/metabolism, tert-Butylhydroperoxide/pharmacology
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8699Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20296)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20296
18637Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20296.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33457Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10412
49381Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 28791)https://www.ccug.se/strain?id=28791
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72603Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13628.1StrainInfo: A central database for resolving microbial strain identifiers
119367Curators of the CIPCollection of Institut Pasteur (CIP 69.5)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2069.5