Strain identifier

BacDive ID: 3082

Type strain: Yes

Species: Corynebacterium bovis

Strain history: CIP <- 1954, NCTC, A.C. Evans

NCBI tax ID(s): 927655 (strain), 36808 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8932

BacDive-ID: 3082

DSM-Number: 20582

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Corynebacterium bovis DSM 20582 is a microaerophile, mesophilic bacterium that was isolated from milk.

NCBI tax id

NCBI tax idMatching level
36808species
927655strain

strain history

@refhistory
8932<- NCTC <- A.C. Evans
360831954, NCTC, A.C. Evans
67770CIP 54.80 <-- A. C. Evans.
116911CIP <- 1954, NCTC, A.C. Evans

doi: 10.13145/bacdive3082.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium bovis
  • full scientific name: Corynebacterium bovis Bergey et al. 1923 (Approved Lists 1980)

@ref: 8932

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium bovis

full scientific name: Corynebacterium bovis Bergey et al. 1923

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no92.401
69480100positive
116911nopositiverod-shaped

colony morphology

@refcolony colorincubation periodmedium used
18762Light ivory10-14 daysISP 2
18762Light ivory10-14 daysISP 4
18762Light ivory10-14 daysISP 5
18762Light ivory10-14 daysISP 6
18762Light ivory10-14 daysISP 7
116911

multicellular morphology

@refforms multicellular complexmedium name
18762noISP 2
18762noISP 4
18762noISP 5
18762noISP 6
18762noISP 7

multimedia

  • @ref: 8932
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_20582.jpg
  • caption: Medium 535a 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18762ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18762ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18762ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18762ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18762ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
8932COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693
8932TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)yesName: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535a
36083MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
116911CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45
116911CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
116911CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
18762positiveoptimum37mesophilic
8932positivegrowth37mesophilic
36083positivegrowth37mesophilic
44576positivegrowth37mesophilic
67770positivegrowth37mesophilic
116911positivegrowth25-41
116911nogrowth10psychrophilic
116911nogrowth15psychrophilic
116911nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44576microaerophile
116911obligate aerobe

spore formation

@refspore formationconfidence
69481no96
69480no99.996

halophily

@refsaltgrowthtested relationconcentration
18762NaClpositivemaximum2.5 %
116911NaClpositivegrowth0-4 %
116911NaClnogrowth6 %
116911NaClnogrowth8 %
116911NaClnogrowth10 %

murein

  • @ref: 8932
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11691116947citrate-carbon source
1169114853esculin-hydrolysis
116911606565hippurate+hydrolysis
11691117632nitrate-reduction
11691116301nitrite-reduction
11691117632nitrate-respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 116911
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 116911
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11691115688acetoin-
11691117234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382lipase (C 14)-
68382esterase (C 4)-
68379catalase+1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-glucuronidase-3.2.1.31
68379pyrazinamidase-3.5.1.B15
116911oxidase-
116911beta-galactosidase-3.2.1.23
116911alcohol dehydrogenase-1.1.1.1
116911gelatinase-
116911amylase-
116911DNase-
116911caseinase-3.4.21.50
116911catalase+1.11.1.6
116911tween esterase-
116911gamma-glutamyltransferase-2.3.2.2
116911lecithinase-
116911lipase-
116911lysine decarboxylase-4.1.1.18
116911ornithine decarboxylase-4.1.1.17
116911protease-
116911urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
18762-------------------
8932--++-+-----+--------+
8932--++-+--------------+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18762-------------------
116911+-+-+----++-+-------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116911++++---------------+------+----+----------------------------+--------------+-+---------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
8932milk
44576Milk
67770Milk
116911Food, MilkWashingtonUnited States of AmericaUSANorth America1930

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Milk
#Engineered#Food production#Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_4469.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_2234;97_2705;98_3358;99_4469&stattab=map
  • Last taxonomy: Corynebacterium bovis subclade
  • 16S sequence: X82051
  • Sequence Identity:
  • Total samples: 21926
  • soil counts: 1636
  • aquatic counts: 1310
  • animal counts: 18724
  • plant counts: 256

Safety information

risk assessment

@refbiosafety levelbiosafety level commentpathogenicity humanpathogenicity animal
187622Risk group (German classification)
89322Risk group (German classification)yesyes
1169112Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Corynebacterium bovis (strain ATCC 7715) gene for16S ribosomal RNAD385751411ena927655
20218Corynebacterium bovis DSM 20582 strain ATCC 7715 16S ribosomal RNA gene, partial sequenceJX4563721289ena927655
20218C.bovis 16S ribosomal DNAX820511387ena927655
8932Corynebacterium bovis partial 16S rRNA gene, strain NCTC 3224X844441445ena927655

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium bovis DSM 20582 = CIP 54.80GCA_014191555contigncbi927655
66792Corynebacterium bovis DSM 20582927655.4wgspatric927655
66792Corynebacterium bovis DSM 20582 = CIP 54.80927655.8wgspatric927655
66792Corynebacterium bovis strain FDAARGOS_105236808.66completepatric36808
66792Corynebacterium bovis DSM 205822824244109draftimg927655
66792Corynebacterium bovis DSM 20582651324023draftimg927655
67770Corynebacterium bovis DSM 20582 = CIP 54.80GCA_000183325contigncbi927655

GC content

@refGC-contentmethod
893268.3
6777069.7thermal denaturation, midpoint method (Tm)
6777068.3thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno96no
gram-positiveyes94.241no
anaerobicno99.538yes
halophileyes79.232no
spore-formingno91.862no
glucose-utilyes88.794no
aerobicno65.308yes
motileno94.369no
flagellatedno97.745no
thermophileno96.95yes
glucose-fermentno60.579no

External links

@ref: 8932

culture collection no.: DSM 20582, ATCC 7715, NCTC 3224, CCUG 2705, JCM 11947, BCRC 12363, CIP 54.80, IMET 10632, JCM 1482, NCDO 1689, NCIMB 701689, PCM 2047

straininfo link

  • @ref: 72600
  • straininfo: 46452

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology15829661Technical note: molecular typing of Corynebacterium bovis isolates by pulsed-field gel electrophoresis.Garcia-Crespo D, Navas J, Gradillas G, Juste RAJ Dairy Sci10.3168/jds.S0022-0302(05)72842-32005Animals, *Bacterial Typing Techniques, Cattle, Corynebacterium/*classification/*genetics, *Electrophoresis, Gel, Pulsed-Field, Female, Genome, Bacterial, Mastitis, Bovine/*microbiology, Milk/microbiology, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Reproducibility of ResultsGenetics
Genetics22843578Draft genome sequence of Corynebacterium bovis DSM 20582, which causes clinical mastitis in dairy cows.Schroder J, Glaub A, Schneider J, Trost E, Tauch AJ Bacteriol10.1128/JB.00839-122012Adaptation, Biological, Animals, Cattle, Corynebacterium/*genetics/isolation & purification, DNA, Bacterial/*chemistry/*genetics, *Genome, Bacterial, Lipid Metabolism, Mastitis, Bovine/microbiology, Metabolic Networks and Pathways/genetics, Milk/metabolism, Molecular Sequence Data, *Sequence Analysis, DNAMetabolism
Phylogeny23849444Pathogenicity and genetic variation of 3 strains of Corynebacterium bovis in immunodeficient mice.Dole VS, Henderson KS, Fister RD, Pietrowski MT, Maldonado G, Clifford CBJ Am Assoc Lab Anim Sci2013Animals, Cattle, Corynebacterium/*classification/genetics/isolation & purification/*pathogenicity, Corynebacterium Infections/diagnosis/microbiology/pathology/*veterinary, DNA, Bacterial/analysis/genetics, Feces/microbiology, Female, Genes, Bacterial, *Genetic Variation, Housing, Animal, *Mice, Mice, Nude, RNA, Ribosomal, 16S/analysis/genetics, Real-Time Polymerase Chain Reaction, Rodent Diseases/diagnosis/microbiology/pathology, Species Specificity, VirulenceEnzymology
Phylogeny30586440Genotypic differences between strains of the opportunistic pathogen Corynebacterium bovis isolated from humans, cows, and rodents.Cheleuitte-Nieves C, Gulvik CA, McQuiston JR, Humrighouse BW, Bell ME, Villarma A, Fischetti VA, Westblade LF, Lipman NSPLoS One10.1371/journal.pone.02092312018Animals, Cattle, Corynebacterium/*genetics/*isolation & purification/pathogenicity, Corynebacterium Infections/microbiology/veterinary, DNA, Circular, Female, Genome, Bacterial, Genotype, Humans, Mice, Nude, Opportunistic Infections/microbiology/veterinary, Phylogeny, RNA, Bacterial, RNA, Ribosomal, 16S, Rats, Virulence Factors/geneticsGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8932Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20582)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20582
18762Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20582.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36083Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18256
44576Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2705)https://www.ccug.se/strain?id=2705
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72600Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46452.1StrainInfo: A central database for resolving microbial strain identifiers
116911Curators of the CIPCollection of Institut Pasteur (CIP 54.80)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2054.80