Strain identifier

BacDive ID: 3081

Type strain: Yes

Species: Gulosibacter faecalis

Strain Designation: B 187

Strain history: CIP <- 2004, IFO <- ATCC 13722

NCBI tax ID(s): 272240 (species)

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General

@ref: 8578

BacDive-ID: 3081

DSM-Number: 20154

keywords: Bacteria, obligate aerobe, mesophilic, Gram-positive, oval-shaped

description: Gulosibacter faecalis B 187 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from cow manure.

NCBI tax id

  • NCBI tax id: 272240
  • Matching level: species

strain history

@refhistory
8578<- ATCC <- H.P. Broquist, B 187
119217CIP <- 2004, IFO <- ATCC 13722

doi: 10.13145/bacdive3081.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Gulosibacter
  • species: Gulosibacter faecalis
  • full scientific name: Gulosibacter faecalis (Lin et al. 2004) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Zimmermannella faecalis

@ref: 8578

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Gulosibacter

species: Gulosibacter faecalis

full scientific name: Gulosibacter faecalis (Lin et al. 2004) Nouioui et al. 2018

strain designation: B 187

type strain: yes

Morphology

cell morphology

  • @ref: 119217
  • gram stain: positive
  • cell shape: oval-shaped
  • motility: no

colony morphology

  • @ref: 119217

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8578TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
33992MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
119217CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
119217CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
8578positivegrowth28mesophilic
33992positivegrowth30mesophilic
119217positivegrowth15-41
119217nogrowth10psychrophilic
119217nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119217
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
119217NaClpositivegrowth0-8 %
119217NaClnogrowth10 %

murein

  • @ref: 8578
  • murein short key: B08
  • type: B1ß {Gly} [L-Hsr] D-Glu-Gly-L-Dab

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11921716947citrate+carbon source
1192174853esculin-hydrolysis
119217606565hippurate-hydrolysis
11921717632nitrate-reduction
11921716301nitrite-reduction
11921717632nitrate-respiration

antibiotic resistance

  • @ref: 119217
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 119217
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11921715688acetoin-
11921717234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
119217oxidase-
119217beta-galactosidase-3.2.1.23
119217alcohol dehydrogenase-1.1.1.1
119217gelatinase-
119217amylase+
119217DNase+
119217caseinase+3.4.21.50
119217catalase+1.11.1.6
119217tween esterase+
119217gamma-glutamyltransferase+2.3.2.2
119217lecithinase-
119217lipase-
119217lysine decarboxylase-4.1.1.18
119217ornithine decarboxylase-4.1.1.17
119217phenylalanine ammonia-lyase-4.3.1.24
119217protease-
119217tryptophan deaminase-
119217urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119217--+--+-----+--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119217++--+------------------+---------+----+----++---+++-++-------+--------++++++--+----------+++----++-

Isolation, sampling and environmental information

isolation

@refsample type
8578cow manure
119217Animal, Cow, feces

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
85781Risk group (German classification)
1192171Risk group (French classification)

Sequence information

GC content

  • @ref: 8578
  • GC-content: 73.7

External links

@ref: 8578

culture collection no.: DSM 20154, NCDO 1718, ATCC 13722, CIP 108568, IAM 15030, IFO 15706, TISTR 1514

literature

  • topic: Phylogeny
  • Pubmed-ID: 15388726
  • title: Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae.
  • authors: Lin YC, Uemori K, de Briel DA, Arunpairojana V, Yokota A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02741-0
  • year: 2004
  • mesh: Actinomycetales/*classification/*isolation & purification/physiology/ultrastructure, Actinomycetales Infections/microbiology, Amino Acids, Diamino/analysis, Animals, Bacterial Typing Techniques, Base Composition, Blood/microbiology, Butter/microbiology, Cattle, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis, Feces/microbiology, Genes, rRNA, Humans, Microscopy, Electron, Scanning, Molecular Sequence Data, Muramic Acids/chemistry, Peptidoglycan/chemistry, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Urine/microbiology, Vitamin K 2/analysis, Wounds and Injuries/microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8578Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20154)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20154
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33992Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6204
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
119217Curators of the CIPCollection of Institut Pasteur (CIP 108568)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108568