Strain identifier
BacDive ID: 3073
Type strain:
Species: Adlercreutzia equolifaciens
Strain Designation: FJC-B9
Strain history: <- CCUG <- Toshinari Maruo, FJC-B9
NCBI tax ID(s): 446660 (species)
General
@ref: 8024
BacDive-ID: 3073
DSM-Number: 19450
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, ovoid-shaped
description: Adlercreutzia equolifaciens FJC-B9 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from human faeces.
NCBI tax id
- NCBI tax id: 446660
- Matching level: species
strain history
@ref | history |
---|---|
8024 | <- Toshinari Maruo; FJC-B9 |
67770 | T. Maruo FJC-B9. |
67771 | <- CCUG <- Toshinari Maruo, FJC-B9 |
doi: 10.13145/bacdive3073.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Coriobacteriia
- order: Eggerthellales
- family: Eggerthellaceae
- genus: Adlercreutzia
- species: Adlercreutzia equolifaciens
- full scientific name: Adlercreutzia equolifaciens Maruo et al. 2008
@ref: 8024
domain: Bacteria
phylum: Actinobacteria
class: Coriobacteriia
order: Coriobacteriales
family: Eggerthellaceae
genus: Adlercreutzia
species: Adlercreutzia equolifaciens subsp. equolifaciens
full scientific name: Adlercreutzia equolifaciens subsp. equolifaciens (Maruo et al. 2008) Nouioui et al. 2018
strain designation: FJC-B9
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
32379 | positive | 2.1 µm | 0.65 µm | ovoid-shaped | no | |
67771 | no | |||||
67771 | positive | |||||
69480 | positive | 100 |
colony morphology
- @ref: 60388
- incubation period: 2-3 days
pigmentation
- @ref: 32379
- production: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8024 | PYG MEDIUM (MODIFIED) (DSMZ Medium 104) | yes | https://mediadive.dsmz.de/medium/104 | Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water |
8024 | WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) | yes | https://mediadive.dsmz.de/medium/339 | Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8024 | positive | growth | 37 | mesophilic |
60388 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
67771 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
8024 | anaerobe | |
32379 | anaerobe | |
60388 | anaerobe | |
67771 | anaerobe | |
69480 | anaerobe | 99.078 |
spore formation
@ref | spore formation | confidence |
---|---|---|
32379 | no | |
67771 | no | |
69481 | no | 100 |
69480 | no | 99.987 |
observation
@ref | observation |
---|---|
67770 | quinones: DMMK-6 |
67771 | quinones: DMMK-6(70-96%), MMK-6(1-29%) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
32379 | 29016 | arginine | + | carbon source |
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | + | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 16634 | raffinose | - | fermentation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68380 | serine arylamidase | - | |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | histidine arylamidase | - | |
68380 | glycin arylamidase | - | |
68380 | alanine arylamidase | - | 3.4.11.2 |
68380 | tyrosine arylamidase | - | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | phenylalanine arylamidase | - | |
68380 | leucyl glycin arylamidase | - | 3.4.11.1 |
68380 | proline-arylamidase | - | 3.4.11.5 |
68380 | L-arginine arylamidase | - | |
68380 | alkaline phosphatase | - | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-glucosidase | - | 3.2.1.21 |
68380 | alpha-glucosidase | - | 3.2.1.20 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | beta-galactosidase | - | 3.2.1.23 |
68380 | alpha-galactosidase | - | 3.2.1.22 |
68380 | arginine dihydrolase | + | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8024 | - | + | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
8024 | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | sampling date |
---|---|---|---|---|---|
8024 | human faeces | Japan | JPN | Asia | |
60388 | Human feces,25-yr-old healthy woman | Japan | JPN | Asia | 2006-06-01 |
67770 | Human feces | ||||
67771 | From human feces | Japan | JPN | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Human | |
#Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
taxonmaps
- @ref: 69479
- File name: preview.99_4556.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15800;96_2274;97_2756;98_3424;99_4556&stattab=map
- Last taxonomy: Adlercreutzia equolifaciens subclade
- 16S sequence: AB649147
- Sequence Identity:
- Total samples: 103294
- soil counts: 562
- aquatic counts: 2542
- animal counts: 99975
- plant counts: 215
Safety information
risk assessment
- @ref: 8024
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Adlercreutzia equolifaciens gene for 16S ribosomal RNA, partial sequence | AB649147 | 1469 | ena | 446660 |
8024 | Adlercreutzia equolifaciens gene for 16S ribosomal RNA, partial sequence, strain: FJC-B9 | AB306661 | 1469 | ena | 446660 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Adlercreutzia equolifaciens DSM 19450 | GCA_000478885 | complete | ncbi | 1384484 |
66792 | Adlercreutzia equolifaciens KCTC 15235 | GCA_003428235 | contig | ncbi | 446660 |
66792 | Adlercreutzia equolifaciens DSM 19450 | 1384484.3 | complete | patric | 1384484 |
66792 | Adlercreutzia equolifaciens strain KCTC 15235 | 446660.3 | wgs | patric | 446660 |
66792 | Adlercreutzia equolifaciens DSM 19450 | 2597489914 | complete | img | 1384484 |
GC content
@ref | GC-content | method |
---|---|---|
8024 | 64.1 | |
32379 | 64 | |
67770 | 64 | high performance liquid chromatography (HPLC) |
67771 | 64.0 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 93.829 | yes |
flagellated | no | 98.49 | yes |
gram-positive | yes | 91.296 | yes |
anaerobic | yes | 98.94 | yes |
aerobic | no | 96.037 | no |
halophile | no | 86.986 | no |
spore-forming | no | 93.116 | no |
glucose-util | yes | 65.38 | no |
thermophile | no | 99.191 | no |
glucose-ferment | no | 59.512 | no |
External links
@ref: 8024
culture collection no.: DSM 19450, CCUG 54925, JCM 14793, KCTC 15235
straininfo link
- @ref: 72592
- straininfo: 406541
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 18450717 | Adlercreutzia equolifaciens gen. nov., sp. nov., an equol-producing bacterium isolated from human faeces, and emended description of the genus Eggerthella. | Maruo T, Sakamoto M, Ito C, Toda T, Benno Y | Int J Syst Evol Microbiol | 10.1099/ijs.0.65404-0 | 2008 | Bacterial Typing Techniques, Cytophagaceae/*classification/genetics/isolation & purification/physiology, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Equol, Feces/*microbiology, Genes, rRNA, Genotype, Humans, Isoflavones/*biosynthesis/metabolism, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity | Genetics |
Genetics | 24051320 | Complete Genome Sequence of the Equol-Producing Bacterium Adlercreutzia equolifaciens DSM 19450T. | Toh H, Oshima K, Suzuki T, Hattori M, Morita H | Genome Announc | 10.1128/genomeA.00742-13 | 2013 | ||
Metabolism | 25747993 | Biotransformation of (-)-epigallocatechin and (-)-gallocatechin by intestinal bacteria involved in isoflavone metabolism. | Takagaki A, Nanjo F | Biol Pharm Bull | 10.1248/bpb.b14-00646 | 2015 | Actinobacteria/*metabolism, Biotransformation, Catechin/*analogs & derivatives/metabolism, Intestines/microbiology, Isoflavones/metabolism | |
Phylogeny | 29583112 | Ellagibacter isourolithinifaciens gen. nov., sp. nov., a new member of the family Eggerthellaceae, isolated from human gut. | Beltran D, Romo-Vaquero M, Espin JC, Tomas-Barberan FA, Selma MV | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002735 | 2018 | Actinobacteria/*classification/genetics/isolation & purification, Adult, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid, Fatty Acids/chemistry, Feces/microbiology, Gastrointestinal Tract/*microbiology, Glycolipids/chemistry, Humans, Male, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Pathogenicity |
Metabolism | 31052328 | Transcriptional Regulation of the Equol Biosynthesis Gene Cluster in Adlercreutzia equolifaciens DSM19450(T). | Florez AB, Vazquez L, Rodriguez J, Redruello B, Mayo B | Nutrients | 10.3390/nu11050993 | 2019 | Actinobacteria/*metabolism, Equol/*biosynthesis/*genetics, Gene Expression Regulation, Bacterial, Genes, Bacterial, Isoflavones/genetics/metabolism, *Multigene Family, *Regulatory Elements, Transcriptional | |
Metabolism | 32586036 | Metabolism of Soy Isoflavones by Intestinal Bacteria: Genome Analysis of an Adlercreutzia Equolifaciens Strain That Does Not Produce Equol. | Vazquez L, Florez AB, Redruello B, Mayo B | Biomolecules | 10.3390/biom10060950 | 2020 | Actinobacteria/classification/*genetics/*metabolism, Equol/biosynthesis, Female, Humans, Intestines/*microbiology, Isoflavones/analysis/genetics/*metabolism | Pathogenicity |
Metabolism | 34173644 | Heterologous expression of equol biosynthesis genes from Adlercreutzia equolifaciens. | Vazquez L, Florez AB, Rodriguez J, Mayo B | FEMS Microbiol Lett | 10.1093/femsle/fnab082 | 2021 | Actinobacteria/*genetics, Bacterial Proteins/*genetics/metabolism, Cloning, Molecular, Equol/*biosynthesis, Escherichia coli/genetics/*metabolism, *Gene Expression, Isoflavones/metabolism | Enzymology |
Phylogeny | 34870581 | Adlercreutzia hattorii sp. nov., an equol non-producing bacterium isolated from human faeces. | Sakamoto M, Ikeyama N, Yuki M, Murakami T, Mori H, Iino T, Ohkuma M | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005121 | 2021 | Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Equol/biosynthesis, Feces/microbiology, Humans, Japan, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Pathogenicity |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed | ID_cross_reference |
---|---|---|---|---|---|---|---|
8024 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19450) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19450 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | ||
32379 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28776041 | 28606 | ||
60388 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 54925) | https://www.ccug.se/strain?id=54925 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | |||||
68380 | Automatically annotated from API rID32A | ||||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
72592 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID406541.1 | StrainInfo: A central database for resolving microbial strain identifiers |