Strain identifier

BacDive ID: 3066

Type strain: Yes

Species: Slackia isoflavoniconvertens

Strain Designation: HE8

Strain history: <- DSM <- A Matthies, Dept. Gastrointestinal Microbiology, German Inst. of Human Nutrition(DIFE), Nuthetal, Germany, HE8

NCBI tax ID(s): 572010 (species)

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General

@ref: 16097

BacDive-ID: 3066

DSM-Number: 22006

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, rod-shaped

description: Slackia isoflavoniconvertens HE8 is an anaerobe, mesophilic, rod-shaped bacterium that was isolated from faeces of a healthy human.

NCBI tax id

  • NCBI tax id: 572010
  • Matching level: species

strain history

@refhistory
16097<- A. Matthies, Dept. Gastrointestinal Microbiology, German Inst. of Human Nutrition (DIFE), Nuthetal, Germany; HE8
67770CCUG 57679 <-- A. Matthies HE8.
67771<- DSM <- A Matthies, Dept. Gastrointestinal Microbiology, German Inst. of Human Nutrition(DIFE), Nuthetal, Germany, HE8

doi: 10.13145/bacdive3066.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Coriobacteriia
  • order: Eggerthellales
  • family: Eggerthellaceae
  • genus: Slackia
  • species: Slackia isoflavoniconvertens
  • full scientific name: Slackia isoflavoniconvertens Matthies et al. 2009

@ref: 16097

domain: Bacteria

phylum: Actinobacteria

class: Coriobacteriia

order: Coriobacteriales

family: Eggerthellaceae

genus: Slackia

species: Slackia isoflavoniconvertens

full scientific name: Slackia isoflavoniconvertens Matthies et al. 2009

strain designation: HE8

type strain: yes

Morphology

cell morphology

@refcell shapegram stain
67771rod-shaped
67771positive

colony morphology

  • @ref: 61414
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16097WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)yeshttps://mediadive.dsmz.de/medium/339Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339; with strain-specific modifications) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l Arginine 10.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water
16097COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
16097positivegrowth37mesophilic
61414positivegrowth37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16097anaerobe
61414anaerobe
67771anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
16097-+---------------------------
16097-+---------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
16097faeces of a healthy humanNuthetalGermanyDEUEurope
61414Human feces,healthy personNuthetalGermanyDEUEurope2004-11-30
67770Human feces
67771From faeces of a healthy humanNuthetalGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 16097
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
16097Kyklioacalles bupleuri isolate ZFMK-DNA JJ0131 28S ribosomal RNA gene, partial sequenceEU286403377ena501657
67770Slackia isoflavoniconvertens gene for 16S ribosomal RNA, partial sequenceAB5664181473ena572010
67770Slackia isoflavoniconvertens strain HE8 16S ribosomal RNA gene, partial sequenceEU8264031339ena572010

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Slackia isoflavoniconvertens DSM 22006GCA_014192995contigncbi572010
66792Slackia isoflavoniconvertens strain DSM 22006572010.87wgspatric572010
66792Slackia isoflavoniconvertens strain DSM 22006572010.5wgspatric572010
66792Slackia isoflavoniconvertens DSM 220062866337646draftimg572010
66792Slackia isoflavoniconvertens DSM 220062824782603draftimg572010
67771Slackia isoflavoniconvertens DSM 22006GCA_003725955contigncbi572010

GC content

  • @ref: 16097
  • GC-content: 58.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes87.876no
anaerobicyes99.245yes
halophileno80.785no
spore-formingno94.37no
glucose-utilyes64.778no
flagellatedno98.891no
aerobicno98.959no
thermophileno93.165yes
motileno94.789no
glucose-fermentno57.115no

External links

@ref: 16097

culture collection no.: DSM 22006, CCUG 57679, KCTC 15165, JCM 16137

straininfo link

  • @ref: 72586
  • straininfo: 406180

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism25747993Biotransformation of (-)-epigallocatechin and (-)-gallocatechin by intestinal bacteria involved in isoflavone metabolism.Takagaki A, Nanjo FBiol Pharm Bull10.1248/bpb.b14-006462015Actinobacteria/*metabolism, Biotransformation, Catechin/*analogs & derivatives/metabolism, Intestines/microbiology, Isoflavones/metabolism
Genetics30643901Draft Genome Sequences of Type Strains of Gordonibacter faecihominis, Paraeggerthella hongkongensis, Parvibacter caecicola,Slackia equolifaciens, Slackia faecicanis, and Slackia isoflavoniconvertens.Danylec N, Stoll DA, Dotsch A, Huch MMicrobiol Resour Announc10.1128/MRA.01532-182019
Pathogenicity32517924Application of recombinant lactic acid bacteria and bifidobacteria able to enrich soy beverage in dihydrodaidzein and dihydrogenistein.Peiroten A, Gaya P, M feminine Landete JFood Res Int10.1016/j.foodres.2020.1092572020Actinobacteria, Bifidobacterium/genetics, Humans, *Isoflavones, *Lactobacillales, *Soy Milk
Biotechnology34281716Heterologous production of equol by lactic acid bacteria strains in culture medium and food.Ruiz de la Bastida A, Peiroten A, Langa S, Arques JL, Landete JMInt J Food Microbiol10.1016/j.ijfoodmicro.2021.1093282021*Actinobacteria, Animals, Cattle, Equol, Female, *Isoflavones, *Lactobacillales, OxidoreductasesEnzymology

Reference

@idauthorscataloguedoi/urltitle
16097Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22006)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22006
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
61414Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 57679)https://www.ccug.se/strain?id=57679
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68380Automatically annotated from API rID32A
72586Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID406180.1StrainInfo: A central database for resolving microbial strain identifiers