Strain identifier

BacDive ID: 3055

Type strain: No

Species: Eggerthella lenta

Strain Designation: MSMC 77-67

Strain history: CIP <- 1994, ATCC <- R.J. Zabransky: strain MSMC 77-67

NCBI tax ID(s): 84112 (species)

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General

@ref: 6014

BacDive-ID: 3055

DSM-Number: 15644

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, human pathogen

description: Eggerthella lenta MSMC 77-67 is an anaerobe, mesophilic human pathogen that was isolated from abdominal wound.

NCBI tax id

  • NCBI tax id: 84112
  • Matching level: species

strain history

@refhistory
6014<- J. Tang, ATCC; ATCC 43055 <- R. J. Zabransky;
120580CIP <- 1994, ATCC <- R.J. Zabransky: strain MSMC 77-67

doi: 10.13145/bacdive3055.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Coriobacteriia
  • order: Eggerthellales
  • family: Eggerthellaceae
  • genus: Eggerthella
  • species: Eggerthella lenta
  • full scientific name: Eggerthella lenta (Eggerth 1935) Wade et al. 1999

  • synonyms

    @refsynonym
    20215Eubacterium lentum
    20215Eggerthella lenta
    20215Bacteroides lentus

@ref: 6014

domain: Bacteria

phylum: Actinobacteria

class: Coriobacteriia

order: Coriobacteriales

family: Eggerthellaceae

genus: Eggerthella

species: Eggerthella lenta

full scientific name: Eggerthella lenta (Eggerth 1935) Wade et al. 1999 emend. Maruo et al. 2008 emend. Nouioui et al. 2018

strain designation: MSMC 77-67

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no95.289
6948099.999positive
120580nonegativerod-shaped

colony morphology

  • @ref: 120580

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6014COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
6014EUBACTERIUM LENTUM MEDIUM (DSMZ Medium 209)yeshttps://mediadive.dsmz.de/medium/209Name: EUBACTERIUM LENTUM MEDIUM (DSMZ Medium 209) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Arginine 5.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Resazurin 0.001 g/l NaOH Vitamin K1 Distilled water
39743MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
120580CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
6014positivegrowth37mesophilic
39743positivegrowth37mesophilic
52315positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
6014anaerobe
52315anaerobe
69480anaerobe99.999
120580anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.975

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1205804853esculin-hydrolysis
12058017632nitrate+reduction
12058016301nitrite-reduction
12058017632nitrate+respiration
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
12058035581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382alkaline phosphatase-3.1.3.1
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5
120580oxidase-
120580beta-galactosidase-3.2.1.23
120580gelatinase-
120580amylase-
120580DNase-
120580caseinase-3.4.21.50
120580catalase-1.11.1.6
120580tween esterase-
120580lecithinase-
120580lipase-
120580protease-
120580urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    52315C12:01.112
    52315C13:00.513
    52315C14:01414
    52315C15:01.215
    52315C16:010.916
    52315C17:00.417
    52315C18:00.418
    52315C13:0 3OH/C15:1 i I/H3.114.469
    52315C13:0 ANTEISO0.412.701
    52315C13:0 iso0.612.612
    52315C13:1 at 12-130.512.931
    52315C14:0 3OH/C16:1 ISO I1.315.485
    52315C14:0 ISO4.513.618
    52315C15:0 ANTEISO5.914.711
    52315C15:0 ISO1.114.621
    52315C16:0 aldehyde5.114.949
    52315C16:0 iso0.415.626
    52315C16:1 ω7c0.715.819
    52315C17:0 anteiso0.216.722
    52315C17:1 ISO I/C16:0 DMA32.416.481
    52315C17:1 ω9c ISO0.416.416
    52315C18:1 ISO H0.317.46
    52315C18:1 ω9c3.117.769
    52315C18:2 ω6,9c/C18:0 ANTE4.517.724
    52315C19:0 ISO0.418.633
    52315C19:1 ISO I1.118.473
    52315Unidentified0.413.94
    52315Unidentified0.314.894
    52315Unidentified0.315.939
    52315Unidentified0.516.11
    52315Unidentified0.418.324
    52315unknown 18.1943.718.194
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
52315--++------++--------
120580-----------+--------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
6014-+---------------------------
6014-+---------------------------
52315-+---------------+-----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6014abdominal woundWisconsinUSAUSANorth America
52315Human wound,abdominal
120580Human, Abdominal wound

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Abdomen
#Host Body-Site#Other#Wound

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
6014yesyes2Risk group (German classification)
1205801Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Eggerthella lenta partial 16S rRNA gene, strain ATCC 43055
  • accession: FM207094
  • length: 383
  • database: ena
  • NCBI tax ID: 84112

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Eggerthella lenta DSM 15644GCA_003340005contigncbi84112
66792Eggerthella lenta strain DSM 1564484112.14wgspatric84112

GC content

  • @ref: 6014
  • GC-content: 62

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno86no
motileno92.959no
flagellatedno98.195no
gram-positiveyes83.238no
anaerobicyes98.392no
halophileno90.631no
spore-formingno87.814no
thermophileno99.753yes
glucose-utilyes66.834no
aerobicno95.563yes
glucose-fermentno59.487no

External links

@ref: 6014

culture collection no.: DSM 15644, ATCC 43055, CCUG 34779, CIP 104211, CCM 4714

straininfo link

  • @ref: 72575
  • straininfo: 46035

literature

topicPubmed-IDtitleauthorsjournalDOIyearmesh
Pathogenicity2229368Eubacterium lentum ATCC 43055, a new reference strain for quality control of anaerobic susceptibility tests.Barry AL, Zabransky RJJ Clin Microbiol10.1128/jcm.28.10.2375-2376.19901990Bacteria, Anaerobic/drug effects, Eubacterium/*drug effects, Evaluation Studies as Topic, Microbial Sensitivity Tests/*standards, Quality Control, Reference Standards
Pathogenicity2280013Quality control criteria for testing the susceptibility of anaerobic bacteria to meropenem.Zabransky RJ, Bayola-Mueller L, Jenkins SG, Pitkin DR, Schreckenberger PC, Celig DM, Spiegel CAJ Clin Microbiol10.1128/jcm.28.12.2804-2805.19901990Bacteria, Anaerobic/*drug effects, Meropenem, Microbial Sensitivity Tests/methods/*standards, Quality Control, Reference Values, Thienamycins/*pharmacology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6014Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15644)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15644
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39743Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16095
52315Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 34779)https://www.ccug.se/strain?id=34779
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
72575Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46035.1StrainInfo: A central database for resolving microbial strain identifiers
120580Curators of the CIPCollection of Institut Pasteur (CIP 104211)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104211