Strain identifier

BacDive ID: 3032

Type strain: Yes

Species: Limnohabitans curvus

Strain Designation: MWH-C5

Strain history: <- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-C5

NCBI tax ID(s): 323423 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 15863

BacDive-ID: 3032

DSM-Number: 21645

keywords: genome sequence, 16S sequence, Bacteria, aerobe, chemoorganotroph, mesophilic, rod-shaped, colony-forming

description: Limnohabitans curvus MWH-C5 is an aerobe, chemoorganotroph, mesophilic bacterium that forms circular colonies and was isolated from water of a deep, oligo-mesotrophic freshwater lake.

NCBI tax id

  • NCBI tax id: 323423
  • Matching level: species

strain history

  • @ref: 15863
  • history: <- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-C5

doi: 10.13145/bacdive3032.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Comamonadaceae
  • genus: Limnohabitans
  • species: Limnohabitans curvus
  • full scientific name: Limnohabitans curvus Hahn et al. 2010

@ref: 15863

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Comamonadaceae

genus: Limnohabitans

species: Limnohabitans curvus

full scientific name: Limnohabitans curvus Hahn et al. 2010

strain designation: MWH-C5

type strain: yes

Morphology

cell morphology

  • @ref: 23095
  • cell length: 1.0-1.5 µm
  • cell width: 0.4-0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorcolony shapemedium usedincubation period
23095unpigmentedcircularnutrient broth soytone yeast extract medium
610683-5 days

Culture and growth conditions

culture medium

@refnamegrowthlink
15863R2A MEDIUM (DSMZ Medium 830)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830.pdf
23095nutrient broth soytone yeast extract mediumyes
23095solidified complex mediayes

culture temp

@refgrowthtypetemperaturerange
15863positivegrowth28mesophilic
23095positivegrowth4.0-34.0
23095mesophilic
61068positivegrowth22psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23095aerobe
23095facultative anaerobe
61068aerobe

nutrition type

  • @ref: 23095
  • type: chemoorganotroph

halophily

@refsaltgrowthtested relationconcentration
23095NaClpositivegrowth0.0-0.5 %(w/v)
23095NaClmaximum0.5 %
23095NaClnogrowth>0.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2309529016arginine-assimilation
2309517750betaine-assimilation
2309517126DL-carnitine-assimilation
2309528300glutamine-assimilation
2309517754glycerol-assimilation
2309529805glycolate-assimilation
2309536655glyoxylate-assimilation
2309527570histidine-assimilation
2309529985L-glutamate-assimilation
2309524996lactate-assimilation
2309515792malonate-assimilation
23095506227N-acetylglucosamine-assimilation
2309530623oxalate-assimilation
2309516452oxaloacetate-assimilation
2309528044phenylalanine-assimilation
2309526271proline-assimilation
2309517822serine-assimilation
2309527922sorbose-assimilation
2309516610spermidine-assimilation
2309527897tryptophan-assimilation
2309524996lactate-carbon source
2309528300glutamine-carbon source
2309517754glycerol-carbon source
2309529805glycolate-carbon source
2309529985L-glutamate-carbon source
2309515971L-histidine-carbon source
2309517295L-phenylalanine-carbon source
2309517203L-proline-carbon source
2309517115L-serine-carbon source
2309516828L-tryptophan-carbon source
2309515792malonate-carbon source
23095506227N-acetylglucosamine-carbon source
2309516452oxaloacetate-carbon source
23095309162-oxoglutarate+assimilation
2309530089acetate+assimilation
2309516947citrate+assimilation
2309512936D-galactose+assimilation
2309516988D-ribose+assimilation
2309525115malate+assimilation
2309516236ethanol+assimilation
2309529806fumarate+assimilation
2309524265gluconate+assimilation
2309517234glucose+assimilation
23095glycerate+assimilation
2309537684mannose+assimilation
2309517272propionate+assimilation
2309515361pyruvate+assimilation
2309530031succinate+assimilation
2309517992sucrose+assimilation
23095309162-oxoglutarate+carbon source
2309516947citrate+carbon source
230958391D-gluconate+carbon source
2309517634D-glucose+carbon source
2309516024D-mannose+carbon source
2309525115malate+carbon source
2309515361pyruvate+carbon source

enzymes

@refvalueactivityec
23095catalase+1.11.1.6
23095cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureisolation procedure
15863water of a deep, oligo-mesotrophic freshwater lakeLake Mondsee (47°50'2.92"N;13°22'25.98"E)AustriaAUTEurope47.834113.3739
23095water column of the oligomesotrophic Lake MondseeSalzkammergutnutrient broth soytone yeast extract (NSY) mediumdilution-acclimatization method (DAM)
61068Freshwater,lakeMondsee,Lake MondseeAustriaAUTEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Aquatic#Lake (large)

taxonmaps

  • @ref: 69479
  • File name: preview.99_23757.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1;96_269;97_2286;98_9808;99_23757&stattab=map
  • Last taxonomy: Comamonadaceae
  • 16S sequence: AJ938026
  • Sequence Identity:
  • Total samples: 226
  • aquatic counts: 182
  • animal counts: 41
  • plant counts: 3

Safety information

risk assessment

  • @ref: 15863
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15863
  • description: Limnohabitans curvus 16S rRNA (partial), tRNA-Ile, tRNA-Ala and 23S rRNA (partial) genes, strain MWH-C5
  • accession: AJ938026
  • length: 2196
  • database: ena
  • NCBI tax ID: 323423

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Limnohabitans curvus MWH-C5GCA_003063475contigncbi323423
66792Limnohabitans curvus strain MWH-C5323423.8wgspatric323423
66792Limnohabitans curvus MWH-C52684622876completeimg323423

GC content

@refGC-contentmethod
1586355.5high performance liquid chromatography (HPLC)
2309555.5

External links

@ref: 15863

culture collection no.: DSM 21645, CCUG 56720, KCTC 42562, CCUG 57770

straininfo link

  • @ref: 72552
  • straininfo: 406109

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19671731Limnohabitans curvus gen. nov., sp. nov., a planktonic bacterium isolated from a freshwater lake.Hahn MW, Kasalicky V, Jezbera J, Brandt U, Jezberova J, Simek KInt J Syst Evol Microbiol10.1099/ijs.0.013292-02009Amino Acids/analysis, Betaproteobacteria/classification/*genetics/isolation & purification/metabolism, Fresh Water/microbiology, Microscopy, Electron, Molecular Sequence Data, Monosaccharides/analysis, Phenotype, Phylogeny, Phytoplankton/classification/genetics/*isolation & purification/metabolism, Sucrose/analysis, Temperature, *Water MicrobiologyEnzymology
Phylogeny20118294Limnohabitans australis sp. nov., isolated from a freshwater pond, and emended description of the genus Limnohabitans.Hahn MW, Kasalicky V, Jezbera J, Brandt U, Simek KInt J Syst Evol Microbiol10.1099/ijs.0.022384-02010Bacterial Typing Techniques, Base Composition, Brazil, Comamonadaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fresh Water/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Ponds/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistryGenetics
Phylogeny34402776Limnohabitans radicicola sp. nov., a slow-growing bacterium isolated from rhizosphere of rice plant and emended description of the genus Limnohabitans.Chhetri G, Kim J, Kim I, Kang M, Seo TInt J Syst Evol Microbiol10.1099/ijsem.0.0049572021Bacterial Typing Techniques, Base Composition, Comamonadaceae/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Oryza, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15863Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21645)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21645
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23095Martin W. Hahn,Vojtech Kasalický,Jan Jezbera,Ulrike Brandt,Jitka Jezberová,Karel Simek10.1099/ijs.0.013292-0Limnohabitans curvus gen. nov., sp. nov., a planktonic bacterium isolated from a freshwater lakeIJSEM 60: 1358-1365 201019671731
61068Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 56720)https://www.ccug.se/strain?id=56720
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72552Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID406109.1StrainInfo: A central database for resolving microbial strain identifiers