Strain identifier

BacDive ID: 3031

Type strain: Yes

Species: Roseateles asaccharophilus

Strain Designation: KIN192

Strain history: <- CECT <- J. Lalucat, Universitat de les Illes Balears, Microbiologia, Departament de Biologia, Palma de Mallorca, Spain <- CCUG <- J. Pinhassi, Dept. Pure and Applied Nat. Scs., Univ. Kalmar, Sweden

NCBI tax ID(s): 582607 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 17840

BacDive-ID: 3031

DSM-Number: 25082

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Roseateles asaccharophilus KIN192 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from freshwater lake.

NCBI tax id

  • NCBI tax id: 582607
  • Matching level: species

strain history

  • @ref: 17840
  • history: <- CECT <- J. Lalucat, Universitat de les Illes Balears, Microbiologia, Departament de Biologia, Palma de Mallorca, Spain <- CCUG <- J. Pinhassi, Dept. Pure and Applied Nat. Scs., Univ. Kalmar, Sweden

doi: 10.13145/bacdive3031.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Comamonadaceae
  • genus: Roseateles
  • species: Roseateles asaccharophilus
  • full scientific name: Roseateles asaccharophilus (Gomila et al. 2010) Liu et al. 2023
  • synonyms

    • @ref: 20215
    • synonym: Kinneretia asaccharophila

@ref: 17840

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Comamonadaceae

genus: Kinneretia

species: Kinneretia asaccharophila

full scientific name: Kinneretia asaccharophila Gomila et al. 2010

strain designation: KIN192

type strain: yes

Morphology

cell morphology

  • @ref: 29355
  • gram stain: negative
  • cell length: 2 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 59805
  • incubation period: 1 day

pigmentation

  • @ref: 29355
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 17840
  • name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1a
  • composition: Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
17840positivegrowth28mesophilic
29355positivegrowth20-37
29355positiveoptimum28.5mesophilic
59805positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 59805
  • oxygen tolerance: aerobe

halophily

  • @ref: 29355
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 0.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
29355620642,3-butanediol+carbon source
2935530089acetate+carbon source
2935517128adipate+carbon source
2935521217L-alaninamide+carbon source
2935516449alanine+carbon source
2935540585alpha-cyclodextrin+carbon source
29355286442-oxopentanoate+carbon source
2935522653asparagine+carbon source
2935523652dextrin+carbon source
2935524265gluconate+carbon source
2935529987glutamate+carbon source
2935517596inosine+carbon source
2935524996lactate+carbon source
2935518401phenylacetate+carbon source
2935528044phenylalanine+carbon source
2935526271proline+carbon source
2935551850methyl pyruvate+carbon source
2935517822serine+carbon source
2935526986threonine+carbon source
2935517748thymidine+carbon source
2935553423tween 40+carbon source
2935553426tween 80+carbon source
2935516704uridine+carbon source
2935527248urocanic acid+carbon source
2935517632nitrate+reduction

enzymes

@refvalueactivityec
29355alkaline phosphatase+3.1.3.1
29355gelatinase+
29355cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
17840freshwater lakeLake KinneretIsraelISRAsia
59805FreshwaterLake KinneretIsraelISRAsia2000-09-11

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Aquatic#Lake (large)

Safety information

risk assessment

  • @ref: 17840
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17840
  • description: Kinneretia asaccharophila strain KIN192 16S ribosomal RNA gene, partial sequence
  • accession: AY136099
  • length: 1386
  • database: ena
  • NCBI tax ID: 582607

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kinneretia asaccharophila DSM 25082GCA_004362405scaffoldncbi582607
66792Kinneretia asaccharophila strain DSM 25082582607.3wgspatric582607
66792Kinneretia asaccharophila DSM 250822770939493draftimg582607

GC content

  • @ref: 17840
  • GC-content: 69.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes91.612no
flagellatedyes92.406no
gram-positiveno98.623yes
anaerobicno98.862no
aerobicyes95.468yes
halophileno96.124yes
spore-formingno93.803no
glucose-utilyes70.646no
thermophileno97.004yes
glucose-fermentno86.637no

External links

@ref: 17840

culture collection no.: DSM 25082, CCUG 53117, CECT 7319

straininfo link

  • @ref: 72551
  • straininfo: 369430

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19661516Kinneretia asaccharophila gen. nov., sp. nov., isolated from a freshwater lake, a member of the Rubrivivax branch of the family Comamonadaceae.Gomila M, Pinhassi J, Falsen E, Moore ERB, Lalucat JInt J Syst Evol Microbiol10.1099/ijs.0.011478-02009Bacterial Typing Techniques, Comamonadaceae/*classification/genetics/*isolation & purification/physiology, DNA Gyrase/genetics, DNA, Ribosomal Spacer/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Israel, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityGenetics
Phylogeny31081749Aquincola rivuli sp. nov., isolated from a freshwater stream.Sheu SY, Hsieh TY, Chen WMInt J Syst Evol Microbiol10.1099/ijsem.0.0034292019Bacterial Typing Techniques, Base Composition, Burkholderiales/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Taiwan, Ubiquinone/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
17840Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25082)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25082
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29355Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2576728776041
59805Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 53117)https://www.ccug.se/strain?id=53117
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
72551Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID369430.1StrainInfo: A central database for resolving microbial strain identifiers