Strain identifier
BacDive ID: 3019
Type strain: 
Species: Xenophilus azovorans
Strain Designation: KF46F
Strain history: <- S. Blümel, Univ. Stuttgart <- T. Leisinger, ETH Zuerich; KF46F
NCBI tax ID(s): 1123504 (strain), 151755 (species)
version 8.1 (current version)
General
@ref: 4942
BacDive-ID: 3019
DSM-Number: 13620
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative
description: Xenophilus azovorans KF46F is an aerobe, mesophilic, Gram-negative bacterium that was isolated from soil.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1123504 | strain |
| 151755 | species |
strain history
- @ref: 4942
- history: <- S. Blümel, Univ. Stuttgart <- T. Leisinger, ETH Zuerich; KF46F
doi: 10.13145/bacdive3019.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Comamonadaceae
- genus: Xenophilus
- species: Xenophilus azovorans
- full scientific name: Xenophilus azovorans Blümel et al. 2001
@ref: 4942
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Comamonadaceae
genus: Xenophilus
species: Xenophilus azovorans
full scientific name: Xenophilus azovorans Blümel et al. 2001
strain designation: KF46F
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.986
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 4942 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
| 4942 | MINERAL MEDIUM WITH VITAMINS (DSMZ Medium 462) | yes | https://mediadive.dsmz.de/medium/462 | Name: MINERAL MEDIUM (BRUNNER) WITH VITAMINS (DSMZ Medium 462) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l Peptone 5.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Meat extract 3.0 g/l Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l Pyridoxamine 0.000125 g/l ZnSO4 x 7 H2O 0.0001 g/l Nicotinic acid 5e-05 g/l Vitamin B12 5e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l MnCl2 x 4 H2O 3e-05 g/l p-Aminobenzoate 2.5e-05 g/l Thiamine-HCl x 2 H2O 2.5e-05 g/l NiCl2 x 6 H2O 2e-05 g/l Calcium pantothenate 1.25e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Biotin 5e-06 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 4942 | positive | growth | 30 | mesophilic |
| 57612 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 57612
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.969 |
compound production
- @ref: 4942
- compound: azoreductase
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 57612 C10:0 2.6 10 57612 C15:0 0.9 15 57612 C16:0 36.6 16 57612 C17:0 0.6 17 57612 C10:0 3OH 3.7 11.423 57612 C16:0 2OH 1.1 17.233 57612 C16:1 2OH 1.2 17.047 57612 C16:1 ω7c 7 15.819 57612 C17:0 CYCLO 20.6 16.888 57612 C18:1 2OH 2.4 19.088 57612 C18:1 ω7c /12t/9t 17.6 17.824 57612 C18:2 ω6,9c/C18:0 ANTE 2.1 17.724 57612 C19:0 CYCLO ω8c 1.7 18.9 57612 C8:0 3OH 1.3 9.385 57612 Unidentified 0.7 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent | sampling date |
|---|---|---|---|---|---|
| 4942 | soil | Switzerland | CHE | Europe | |
| 57612 | Soil after enrichment | Switzerland | CHE | Europe | 1980 |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_5654.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1;96_2720;97_3343;98_4214;99_5654&stattab=map
- Last taxonomy: Xenophilus azovorans subclade
- 16S sequence: AF285414
- Sequence Identity:
- Total samples: 78
- soil counts: 27
- aquatic counts: 35
- animal counts: 12
- plant counts: 4
Safety information
risk assessment
- @ref: 4942
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20218
- description: Xenophilus azovorans 16S ribosomal RNA gene, partial sequence
- accession: AF285414
- length: 1484
- database: ena
- NCBI tax ID: 151755
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Xenophilus azovorans DSM 13620 | GCA_000745855 | scaffold | ncbi | 1123504 |
| 66792 | Xenophilus azovorans DSM 13620 | 1123504.3 | wgs | patric | 1123504 |
| 66792 | Xenophilus azovorans DSM 13620 | 2571042002 | draft | img | 1123504 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 83.557 | no |
| flagellated | no | 94.981 | no |
| gram-positive | no | 98.149 | no |
| anaerobic | no | 98.816 | yes |
| aerobic | yes | 93.78 | yes |
| halophile | no | 95.725 | no |
| spore-forming | no | 94.896 | no |
| glucose-util | no | 57.538 | no |
| thermophile | no | 99.316 | yes |
| glucose-ferment | no | 89.65 | no |
External links
@ref: 4942
culture collection no.: DSM 13620, CCUG 47268, NCIMB 13707, ATCC BAA 794
straininfo link
- @ref: 72541
- straininfo: 49497
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 11594616 | Xenophilus azovorans gen. nov., sp. nov., a soil bacterium that is able to degrade azo dyes of the Orange II type. | Blumel S, Busse HJ, Stolz A, Kampfer P | Int J Syst Evol Microbiol | 10.1099/00207713-51-5-1831 | 2001 | Azo Compounds/*metabolism, Benzenesulfonates/*metabolism, Betaproteobacteria/chemistry/*classification/genetics/physiology, Biodegradation, Environmental, DNA, Ribosomal/analysis, Fatty Acids/analysis, Lipid Metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology | Metabolism |
| Enzymology | 12147495 | Molecular cloning and characterization of the gene coding for the aerobic azoreductase from Xenophilus azovorans KF46F. | Blumel S, Knackmuss HJ, Stolz A | Appl Environ Microbiol | 10.1128/AEM.68.8.3948-3955.2002 | 2002 | Aerobiosis, Amino Acid Sequence, Azo Compounds/chemistry/metabolism, Base Sequence, Betaproteobacteria/*enzymology/genetics/growth & development, *Cloning, Molecular, DNA, Bacterial/analysis, Molecular Sequence Data, *NADH, NADPH Oxidoreductases/chemistry/genetics/isolation & purification/metabolism, Nitroreductases, Sequence Analysis, DNA, Substrate Specificity | Metabolism |
| Phylogeny | 19651735 | Xenophilus aerolatus sp. nov., isolated from air. | Kim SJ, Kim YS, Weon HY, Anandham R, Noh HJ, Kwon SW | Int J Syst Evol Microbiol | 10.1099/ijs.0.013185-0 | 2009 | *Air Microbiology, Base Composition, Base Sequence, Comamonadaceae/*classification/genetics/physiology, DNA, Bacterial/chemistry, Korea, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, RNA | Genetics |
| Enzymology | 20508929 | Characterisation of the flavin-free oxygen-tolerant azoreductase from Xenophilus azovorans KF46F in comparison to flavin-containing azoreductases. | Burger S, Stolz A | Appl Microbiol Biotechnol | 10.1007/s00253-010-2669-1 | 2010 | Amino Acid Sequence, Bacteria/chemistry/enzymology/genetics, Bacterial Proteins/*chemistry/genetics/*metabolism, Comamonadaceae/chemistry/*enzymology/genetics, Flavins/*metabolism, Kinetics, Molecular Sequence Data, NADH, NADPH Oxidoreductases/*chemistry/genetics/*metabolism, Nitroreductases, Oxygen/*metabolism, Sequence Alignment, Substrate Specificity | Metabolism |
| Phylogeny | 26296923 | Corticibacter populi gen. nov., sp. nov., a new member of the family Comamonadaceae, from the bark of Populus euramericana. | Fang W, Li Y, Xue H, Tian G, Wang L, Guo MW, Piao CG | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000418 | 2015 | Bacterial Typing Techniques, Base Composition, China, Comamonadaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Endophytes/classification/genetics/isolation & purification, Fatty Acids/chemistry, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, Plant Bark/*microbiology, Populus/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry | Enzymology |
| Genetics | 32226704 | Draft genome sequence of Kocuria indica DP-K7, a methyl red degrading actinobacterium. | Kumaran S, Ngo ACR, Schultes FPJ, Tischler D | 3 Biotech | 10.1007/s13205-020-2136-3 | 2020 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 4942 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13620) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13620 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 57612 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 47268) | https://www.ccug.se/strain?id=47268 | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 72541 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49497.1 | StrainInfo: A central database for resolving microbial strain identifiers |