Strain identifier

BacDive ID: 3

Type strain: No

Species: Acetobacter cerevisiae

Strain Designation: B-468, A8

Strain history: CIP <- 1986, IAM <- T. Asai: strain A8 <- IFO <- ATCC <- NRRL: strain B-468 <- NCTC <- Inst. Garungsgewerber, Germany, Acetobacter acetosus

NCBI tax ID(s): 178900 (species)

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General

@ref: 731

BacDive-ID: 3

DSM-Number: 2324

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Acetobacter cerevisiae B-468 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from Manufacture of vinegar.

NCBI tax id

  • NCBI tax id: 178900
  • Matching level: species

strain history

@refhistory
731<- NCIB <- NCTC <- Inst. Gärungsgewerbe, Berlin (Acetobacter acetosum). According to (8573) this is a strains of Acetobacter orleanensis, but MADLI-TOF and 16S rRNA gene sequencing indicate it is a strain of Acetobacter ceriviseae (19104)
67770IAM 1803 <-- T. Asai A-8 ("Acetobacter acetosus") <-- IFO 3170 <-- ATCC 6438 <-- NRRL B-468 <-- NCTC 2224.
121618CIP <- 1986, IAM <- T. Asai: strain A8 <- IFO <- ATCC <- NRRL: strain B-468 <- NCTC <- Inst. Garungsgewerber, Germany, Acetobacter acetosus

doi: 10.13145/bacdive3.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Acetobacter
  • species: Acetobacter cerevisiae
  • full scientific name: Acetobacter cerevisiae Cleenwerck et al. 2002

@ref: 731

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Acetobacter

species: Acetobacter cerevisiae

full scientific name: Acetobacter cerevisiae Cleenwerck et al. 2002

strain designation: B-468, A8

type strain: no

Morphology

cell morphology

  • @ref: 121618
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
731GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105)yeshttps://mediadive.dsmz.de/medium/105Name: GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105) Composition: Glucose 100.0 g/l CaCO3 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Distilled water
33565MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffiiyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (5.000 g);Peptone (3.000 g);Mannitol (25.000 g)
121618CIP Medium 1yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=1

culture temp

@refgrowthtypetemperaturerange
731positivegrowth26mesophilic
33565positivegrowth30mesophilic
67770positivegrowth25mesophilic
121618positivegrowth10-30
121618nogrowth37mesophilic
121618nogrowth41thermophilic
121618nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121618
  • oxygen tolerance: obligate aerobe

compound production

@refcompound
731acetic acid
731vinegar

halophily

@refsaltgrowthtested relationconcentration
121618NaClpositivegrowth0 %
121618NaClnogrowth2 %
121618NaClnogrowth4 %
121618NaClnogrowth6 %
121618NaClnogrowth8 %
121618NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: Q-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
121618606565hippurate+hydrolysis
12161817632nitrate+reduction
12161816301nitrite-reduction

antibiotic resistance

  • @ref: 121618
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 121618
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
121618oxidase-
121618beta-galactosidase-3.2.1.23
121618gelatinase-
121618catalase+1.11.1.6
121618gamma-glutamyltransferase-2.3.2.2
121618urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121618--++-++---++-----+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121618+--------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample type
67770Manufacture of vinegar
121618Food, Vinegar

taxonmaps

  • @ref: 69479
  • File name: preview.99_1214.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_152;97_827;98_961;99_1214&stattab=map
  • Last taxonomy: Acetobacter
  • 16S sequence: JF793953
  • Sequence Identity:
  • Total samples: 1514
  • soil counts: 185
  • aquatic counts: 172
  • animal counts: 932
  • plant counts: 225

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
7311Risk group (German classification)
1216181Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acetobacter pasteurianus strain DSM 2324 16S ribosomal RNA gene, partial sequenceGQ2406411325ena438
731Acetobacter cerevisiae strain LMG 1592 16S ribosomal RNA gene, partial sequenceJF7939531350ena178900

GC content

@refGC-contentmethod
73157.5
6777058high performance liquid chromatography (HPLC)

External links

@ref: 731

culture collection no.: DSM 2324, ATCC 6438, LMG 1592, NCIB 2224, NRRL B-468, JCM 20269, CCM 2363, CIP 102720, IAM 1803, IFO 3170, LMG 1643, NBRC 3170, NCIMB 2224, NCTC 2224, IMET 10613

straininfo link

  • @ref: 69688
  • straininfo: 15009

literature

  • topic: Phylogeny
  • Pubmed-ID: 19542117
  • title: Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii.
  • authors: Cleenwerck I, De Wachter M, Gonzalez A, De Vuyst L, De Vos P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.005157-0
  • year: 2009
  • mesh: Acetobacteraceae/*classification/genetics/isolation & purification, *Amplified Fragment Length Polymorphism Analysis, *Bacterial Typing Techniques, DNA Fingerprinting/*methods, DNA, Bacterial/analysis, DNA, Ribosomal Spacer/analysis, Deoxyribonucleases, Type II Site-Specific/metabolism, Food Microbiology, Genes, rRNA, Genotype, Gluconacetobacter/*classification/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
731Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2324)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2324
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33565Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14438
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69688Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID15009.1StrainInfo: A central database for resolving microbial strain identifiers
121618Curators of the CIPCollection of Institut Pasteur (CIP 102720)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102720