Strain identifier

BacDive ID: 2985

Type strain: Yes

Species: Polaromonas cryoconiti

Strain Designation: Cr4-35

Strain history: <- R. Margesin, Inst. Microbiology, Univ. Innsbruck, Austria; Cr4-35

NCBI tax ID(s): 866565 (species)

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General

@ref: 17619

BacDive-ID: 2985

DSM-Number: 24278

keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, rod-shaped

description: Polaromonas cryoconiti Cr4-35 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from glacier cryoconite.

NCBI tax id

  • NCBI tax id: 866565
  • Matching level: species

strain history

  • @ref: 17619
  • history: <- R. Margesin, Inst. Microbiology, Univ. Innsbruck, Austria; Cr4-35

doi: 10.13145/bacdive2985.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Comamonadaceae
  • genus: Polaromonas
  • species: Polaromonas cryoconiti
  • full scientific name: Polaromonas cryoconiti Margesin et al. 2012

@ref: 17619

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Comamonadaceae

genus: Polaromonas

species: Polaromonas cryoconiti

full scientific name: Polaromonas cryoconiti Margesin et al. 2012

strain designation: Cr4-35

type strain: yes

Morphology

cell morphology

  • @ref: 23091
  • gram stain: negative
  • cell length: 1.1-1.4 µm
  • cell width: 0.7-0.9 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refincubation periodcolony sizecolony colorcolony shapemedium used
176193-7 days
2309121 days<1.0 mmcreamy whitecircularR2A agar

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
23091Nutrient agar (NA)yes
23091Reasoner's 2A agar (R2A)yescontaining (l-1) 0.5 g yeast extract, 0.5 g peptone, 0.5 g Casamino acids, 0.5 g glucose, 0.5 g starch, 0.3 g sodium pyruvate, 0.3 g K2HPO4, 0.05 g MgSO4 and 15.0 g agar (pH 7)
23091tryptone soy agaryes
17619R2A MEDIUM (DSMZ Medium 830)yesName: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled waterhttps://mediadive.dsmz.de/medium/830

culture temp

@refgrowthtypetemperaturerange
17619positivegrowth20psychrophilic
23091positivegrowth1.0-25.0
23091nogrowth30.0mesophilic
23091positivegrowth1.0psychrophilic
23091positivegrowth1.0-10.0psychrophilic

culture pH

@refabilitytypepH
23091positiveoptimum7.0
23091positivegrowth6.0
23091nogrowth8.0

Physiology and metabolism

oxygen tolerance

  • @ref: 23091
  • oxygen tolerance: aerobe

halophily

  • @ref: 23091
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 1.0

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2309116947citrate-carbon source
2309127897tryptophan-energy source
2309127613amygdalin-fermentation
2309117634D-glucose-fermentation
2309116899D-mannitol-fermentation
2309117268myo-inositol-fermentation
2309130849L-arabinose-fermentation
2309162345L-rhamnose-fermentation
2309128053melibiose-fermentation
2309130911sorbitol-fermentation
2309117992sucrose-fermentation
2309127689decanoate-growth
2309117108D-arabinose-growth
2309117634D-glucose-growth
2309116899D-mannitol-growth
2309116024D-mannose-growth
2309117924D-sorbitol-growth
2309130849L-arabinose-growth
2309117306maltose-growth
2309117268myo-inositol-growth
23091506227N-acetylglucosamine-growth
2309118401phenylacetate-growth
2309117814salicin-growth
2309153258sodium citrate-growth
2309117992sucrose-growth
230914853esculin-hydrolysis
230915291gelatin-hydrolysis
2309117128adipate+growth
2309125115malate+growth
2309132032potassium gluconate+growth
2309153423tween 40+growth
2309153426tween 80+growth
2309117632nitrate+reduction

metabolite production

@refChebi-IDmetaboliteproduction
2309135581indoleno
2309116136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole testcitrate test
2309135581indole-
2309116947citrate-

enzymes

@refvalueactivityec
23091acid phosphatase+3.1.3.2
23091alkaline phosphatase+3.1.3.1
23091alpha-chymotrypsin-3.4.21.1
23091alpha-fucosidase-3.2.1.51
23091alpha-galactosidase-3.2.1.22
23091alpha-glucosidase-3.2.1.20
23091alpha-mannosidase-3.2.1.24
23091amylase-
23091arginine dihydrolase-3.5.3.6
23091beta-galactosidase-3.2.1.23
23091beta-glucosidase-3.2.1.21
23091beta-glucuronidase-3.2.1.31
23091catalase+1.11.1.6
23091cytochrome oxidase+1.9.3.1
23091esterase lipase (C 8)+
23091leucine arylamidase+3.4.11.1
23091lysine decarboxylase-4.1.1.18
23091N-acetyl-beta-glucosaminidase-3.2.1.52
23091naphthol-AS-BI-phosphohydrolase+
23091ornithine decarboxylase-4.1.1.17
23091trypsin-3.4.21.4
23091tryptophan deaminase-4.1.99.1
23091urease-3.5.1.5
23091valine arylamidase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17619glacier cryoconiteHohe Tauern, GroßglocknerAustriaAUTEurope
23091alpine glacier cryoconitePasterze/Großglockner glacier in the Hohe Tauern (at an altitude of 2200 m above sea level)

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Glacier
#Condition#Psychrophilic (<10°C)
#Climate#Cold#Alpine
#Environmental#Terrestrial#Geologic

Safety information

risk assessment

  • @ref: 17619
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17619
  • description: Polaromonas cryoconiti strain Cr4-35 16S ribosomal RNA gene, partial sequence
  • accession: HM583567
  • length: 1491
  • database: ena
  • NCBI tax ID: 866565

GC content

  • @ref: 17619
  • GC-content: 60.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 17619

culture collection no.: DSM 24278, KACC 15090, LMG 26050

straininfo link

  • @ref: 72507
  • straininfo: 404209

literature

  • topic: Phylogeny
  • Pubmed-ID: 22199222
  • title: Polaromonas glacialis sp. nov. and Polaromonas cryoconiti sp. nov., isolated from alpine glacier cryoconite.
  • authors: Margesin R, Sproer C, Zhang DC, Busse HJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.037556-0
  • year: 2011
  • mesh: Austria, Bacterial Typing Techniques, Base Composition, Comamonadaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/analysis, Ice Cover/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, Putrescine/analogs & derivatives/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analysis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17619Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24278)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24278
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23091Rosa Margesin,Cathrin Spröer,De-Chao Zhang,Hans-Jürgen Busse10.1099/ijs.0.037556-0Polaromonas glacialis sp. nov. and Polaromonas cryoconiti sp. nov., isolated from alpine glacier cryoconiteIJSEM 62: 2662-2668 201222199222
72507Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID404209.1StrainInfo: A central database for resolving microbial strain identifiers