Strain identifier
BacDive ID: 2863
Type strain:
Species: Alkaliphilus oremlandii
Strain Designation: OhILAs
Strain history: <- J. F. Stolz, Duquesne Univ., Dept. Biol. Sci., Pittsburgh, USA; OhILAs <- A. Down
NCBI tax ID(s): 461876 (species)
General
@ref: 15923
BacDive-ID: 2863
DSM-Number: 21761
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic
description: Alkaliphilus oremlandii OhILAs is an anaerobe, mesophilic bacterium that was isolated from sediments.
NCBI tax id
- NCBI tax id: 461876
- Matching level: species
strain history
- @ref: 15923
- history: <- J. F. Stolz, Duquesne Univ., Dept. Biol. Sci., Pittsburgh, USA; OhILAs <- A. Down
doi: 10.13145/bacdive2863.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Alkaliphilus
- species: Alkaliphilus oremlandii
- full scientific name: Alkaliphilus oremlandii Fisher et al. 2009
@ref: 15923
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Alkaliphilus
species: Alkaliphilus oremlandii
full scientific name: Alkaliphilus oremlandii Fisher et al. 2009
strain designation: OhILAs
type strain: yes
Culture and growth conditions
culture medium
- @ref: 15923
- name: PY + X MEDIUM (DSMZ Medium 104b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/104b
- composition: Name: PY + X MEDIUM (DSMZ Medium 104b; with strain-specific modifications) Composition: Yeast extract 10.0 g/l D-Fructose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
- @ref: 15923
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 15923
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 15923
- sample type: sediments
- geographic location: Ohio river
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Sediment
taxonmaps
- @ref: 69479
- File name: preview.99_5727.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_191;96_2748;97_3380;98_4263;99_5727&stattab=map
- Last taxonomy: Alkaliphilus oremlandii subclade
- 16S sequence: DQ250645
- Sequence Identity:
- Total samples: 6734
- soil counts: 991
- aquatic counts: 751
- animal counts: 4682
- plant counts: 310
Safety information
risk assessment
- @ref: 15923
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15923
- description: Clostridium sp. OhILAs 16S ribosomal RNA gene, partial sequence
- accession: DQ250645
- length: 1314
- database: ena
- NCBI tax ID: 350688
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Alkaliphilus oremlandii OhILAs | GCA_000018325 | complete | ncbi | 350688 |
66792 | Alkaliphilus oremlandii OhILAs | 350688.5 | complete | patric | 350688 |
66792 | Alkaliphilus oremlandii OhILAs | 641228475 | complete | img | 350688 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 91.118 | no |
flagellated | no | 60.693 | no |
gram-positive | yes | 64.234 | no |
anaerobic | yes | 98.377 | yes |
aerobic | no | 96.464 | yes |
halophile | no | 88.318 | no |
spore-forming | yes | 91.891 | no |
glucose-util | yes | 81.656 | no |
thermophile | no | 98.771 | yes |
glucose-ferment | no | 60.426 | no |
External links
@ref: 15923
culture collection no.: DSM 21761, ATCC BAA1360
straininfo link
- @ref: 72387
- straininfo: 298317
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Metabolism | 18378595 | Transformation of inorganic and organic arsenic by Alkaliphilus oremlandii sp. nov. strain OhILAs. | Fisher E, Dawson AM, Polshyna G, Lisak J, Crable B, Perera E, Ranganathan M, Thangavelu M, Basu P, Stolz JF | Ann N Y Acad Sci | 10.1196/annals.1419.006 | 2008 | Arsenic/*metabolism, Arsenicals/*metabolism, Clostridium/cytology/growth & development/isolation & purification/*metabolism, Culture Media, Kinetics, Roxarsone/metabolism | Enzymology |
Metabolism | 18767149 | The selenoproteome of Clostridium sp. OhILAs: characterization of anaerobic bacterial selenoprotein methionine sulfoxide reductase A. | Kim HY, Zhang Y, Lee BC, Kim JR, Gladyshev VN | Proteins | 10.1002/prot.22212 | 2009 | Amino Acid Sequence, Bacterial Proteins/*chemistry/genetics/metabolism, Base Sequence, Clostridium/classification/*metabolism, Codon, Terminator/chemistry/metabolism, Escherichia coli/genetics/metabolism, Methionine Sulfoxide Reductases, Molecular Sequence Data, Oxidoreductases/*chemistry/genetics/metabolism, Proteome/*metabolism, Selenocysteine/chemistry/metabolism, Selenoproteins/*chemistry/genetics/metabolism | Enzymology |
Metabolism | 21069144 | A proteome investigation of roxarsone degradation by Alkaliphilus oremlandii strain OhILAs. | Chovanec P, Stolz JF, Basu P | Metallomics | 10.1039/b915479e | 2009 | Aldehyde Oxidoreductases/chemistry/metabolism, Amino Acid Sequence, Bacterial Proteins/biosynthesis/chemistry/*metabolism, Biotransformation, Clostridium/enzymology/*metabolism, Culture Media, Electrophoresis, Gel, Two-Dimensional, Molecular Sequence Data, Proteome/chemistry/drug effects/*metabolism, Roxarsone/*metabolism/pharmacokinetics/pharmacology, Sequence Alignment, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | Enzymology |
Metabolism | 21210868 | Tandem use of selenocysteine: adaptation of a selenoprotein glutaredoxin for reduction of selenoprotein methionine sulfoxide reductase. | Kim MJ, Lee BC, Jeong J, Lee KJ, Hwang KY, Gladyshev VN, Kim HY | Mol Microbiol | 10.1111/j.1365-2958.2010.07500.x | 2011 | Amino Acid Sequence, Bacterial Proteins/chemistry/genetics/*metabolism, Clostridium/chemistry/*enzymology/genetics, Escherichia coli/genetics/metabolism, Glutaredoxins/chemistry/genetics/*metabolism, Kinetics, Methionine Sulfoxide Reductases/genetics/*metabolism, Molecular Sequence Data, Oxidation-Reduction, Protein Engineering, Selenocysteine/metabolism, Selenoproteins/genetics/*metabolism, Sequence Alignment | Enzymology |
Metabolism | 25138597 | Microbial degradation of linseed oil-based elastomer and subsequent accumulation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. | Pramanik N, Das R, Rath T, Kundu PP | Appl Biochem Biotechnol | 10.1007/s12010-014-1061-5 | 2014 | Elastomers/chemistry/*metabolism, Gram-Positive Endospore-Forming Rods/*metabolism, Linseed Oil/chemistry/*metabolism, Magnetic Resonance Spectroscopy, Polyesters/chemistry/*metabolism, Spectroscopy, Fourier Transform Infrared | |
Metabolism | 25142266 | Mapping the protein profile involved in the biotransformation of organoarsenicals using an arsenic metabolizing bacterium. | Thomas JA, Chovanec P, Stolz JF, Basu P | Metallomics | 10.1039/c4mt00185k | 2014 | Arsanilic Acid/metabolism, Arsenates/metabolism, Arsenicals/*metabolism, Bacterial Proteins/*metabolism, Biotransformation, Clostridium/growth & development/*metabolism, Proteomics, Roxarsone/metabolism, Tandem Mass Spectrometry | Proteome |
Metabolism | 25635348 | Arsenic biotransformation in solid waste residue: comparison of contributions from bacteria with arsenate and iron reducing pathways. | Tian H, Shi Q, Jing C | Environ Sci Technol | 10.1021/es504618x | 2015 | Arsenates/*metabolism, Arsenic/analysis/*metabolism, Biotransformation/physiology, China, Iron/metabolism, Metallurgy, Oxidation-Reduction, Pantoea/*metabolism, Shewanella/*metabolism, Solid Waste/*analysis, Sulfides/metabolism, X-Ray Absorption Spectroscopy |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
15923 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21761) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21761 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
72387 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID298317.1 | StrainInfo: A central database for resolving microbial strain identifiers |