Strain identifier
BacDive ID: 2852
Type strain: 
Species: Clostridium sulfidigenes
Strain Designation: SGB2
Strain history: <- A. Sallam; SGB2
NCBI tax ID(s): 318464 (species)
version 8.1 (current version)
General
@ref: 7813
BacDive-ID: 2852
DSM-Number: 18982
keywords: 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped
description: Clostridium sulfidigenes SGB2 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from pond sediment.
NCBI tax id
- NCBI tax id: 318464
- Matching level: species
strain history
- @ref: 7813
- history: <- A. Sallam; SGB2
doi: 10.13145/bacdive2852.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium sulfidigenes
- full scientific name: Clostridium sulfidigenes Sallam and Steinbüchel 2009
@ref: 7813
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Clostridium
species: Clostridium sulfidigenes
full scientific name: Clostridium sulfidigenes Sallam and Steinbüchel 2009
strain designation: SGB2
type strain: yes
Morphology
cell morphology
- @ref: 29052
- gram stain: positive
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
- @ref: 7813
- name: PY + X MEDIUM (DSMZ Medium 104b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/104b
- composition: Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 7813 | positive | growth | 35 | mesophilic |
| 29052 | positive | growth | 18-48 | |
| 29052 | positive | optimum | 34 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 29052 | positive | growth | 5.5-9.0 | alkaliphile |
| 29052 | positive | optimum | 6.6 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 7813 | anaerobe |
| 29052 | anaerobe |
spore formation
- @ref: 29052
- spore formation: yes
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 29052 | 16449 | alanine | + | carbon source |
| 29052 | 29016 | arginine | + | carbon source |
| 29052 | 5291 | gelatin | + | carbon source |
| 29052 | 25017 | leucine | + | carbon source |
| 29052 | 18257 | ornithine | + | carbon source |
| 29052 | 28044 | phenylalanine | + | carbon source |
| 29052 | 26271 | proline | + | carbon source |
| 29052 | 15361 | pyruvate | + | carbon source |
| 29052 | 17822 | serine | + | carbon source |
| 29052 | 26986 | threonine | + | carbon source |
metabolite production
- @ref: 29052
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: yes
Isolation, sampling and environmental information
isolation
- @ref: 7813
- sample type: pond sediment
- geographic location: Münster
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Pond (small) |
| #Environmental | #Aquatic | #Sediment |
Safety information
risk assessment
- @ref: 7813
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7813
- description: Clostridium sulfidigenes strain SGB2 16S ribosomal RNA gene, complete sequence
- accession: EF199998
- length: 1504
- database: ena
- NCBI tax ID: 318464
GC content
| @ref | GC-content | method |
|---|---|---|
| 7813 | 32.3 | high performance liquid chromatography (HPLC) |
| 29052 | 32.3 |
External links
@ref: 7813
culture collection no.: DSM 18982, ATCC BAA–1538
straininfo link
- @ref: 72376
- straininfo: 396528
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 19542123 | Clostridium sulfidigenes sp. nov., a mesophilic, proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from pond sediment. | Sallam A, Steinbuchel A | Int J Syst Evol Microbiol | 10.1099/ijs.0.004986-0 | 2009 | Bacterial Proteins, Bacterial Typing Techniques, Base Composition, Clostridium/*classification/genetics/isolation & purification/metabolism, DNA, Bacterial/analysis, Fresh Water/*microbiology, Genes, rRNA, Genotype, Geologic Sediments/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Peptide Hydrolases/*metabolism, Phenotype, Phylogeny, Plant Proteins/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Sulfur-Reducing Bacteria/*classification/genetics/isolation & purification, Thiosulfates/*metabolism | Metabolism |
| Phylogeny | 25230767 | Clostridium huakuii sp. nov., an anaerobic, acetogenic bacterium isolated from methanogenic consortia. | Ruan Z, Wang Y, Zhang C, Song J, Zhai Y, Zhuang Y, Wang H, Chen X, Li Y, Zhao B, Zhao B | Int J Syst Evol Microbiol | 10.1099/ijs.0.062711-0 | 2014 | Bacterial Typing Techniques, Base Composition, *Biofuels, China, Clostridium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Genetics |
| Phylogeny | 26419229 | Clostridium punense sp. nov., an obligate anaerobe isolated from healthy human faeces. | Lanjekar VB, Marathe NP, Shouche YS, Ranade DR | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000644 | 2015 | Bacterial Typing Techniques, Base Composition, Clostridium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Fermentation, Humans, India, Male, Middle Aged, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Pathogenicity |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 7813 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18982) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18982 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 29052 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25482 | 28776041 | ||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 72376 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID396528.1 | StrainInfo: A central database for resolving microbial strain identifiers |