General

@ref: 4847

BacDive-ID: 2826

DSM-Number: 12857

keywords: 16S sequence, Bacteria, anaerobe, thermophilic

description: Lacrimispora amygdalina BR-10 is an anaerobe, thermophilic bacterium that was isolated from sludge from anaerobic wastewater treatment plant.

NCBI tax id

• NCBI tax id: 253257
• Matching level: species

strain history

• @ref: 4847
• history: <- S. N. Parshina, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; BR-10 <- S. N. Parshina {1998}

doi: 10.13145/bacdive2826.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacillota
• domain: Bacteria
• phylum: Bacillota
• class: Clostridia
• order: Eubacteriales
• family: Lachnospiraceae
• genus: Lacrimispora
• species: Lacrimispora amygdalina
• full scientific name: Lacrimispora amygdalina (Parshina et al. 2003) Haas and Blanchard 2020

• synonyms

  • @ref: 20215
  • synonym: Clostridium amygdalinum

@ref: 4847

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Lacrimispora

species: Lacrimispora amygdalina

full scientific name: Lacrimispora amygdalina (Parshina et al. 2003) Haas and Blanchard 2020

strain designation: BR-10

type strain: yes

Culture and growth conditions

culture medium

• @ref: 4847
• name: PY + X MEDIUM (DSMZ Medium 104b)
• growth: yes
• link: https://mediadive.dsmz.de/medium/104b
• composition: Name: PY + X MEDIUM (DSMZ Medium 104b; with strain-specific modifications) Composition: Yeast extract 10.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

• @ref: 4847
• growth: positive
• type: growth
• temperature: 45
• range: thermophilic

Physiology and metabolism

oxygen tolerance

• @ref: 4847
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 4847
• sample type: sludge from anaerobic wastewater treatment plant
• country: Netherlands
• origin.country: NLD
• continent: Europe

isolation source categories

Safety information

risk assessment

• @ref: 4847
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 4847
• description: Clostridium amygdalinum 16S ribosomal RNA gene, partial sequence
• accession: AY353957
• length: 1485
• database: ena
• NCBI tax ID: 253257

GC content

• @ref: 4847
• GC-content: 32.5

External links

@ref: 4847

culture collection no.: DSM 12857, ATCC BAA 501

straininfo link

• @ref: 72350
• straininfo: 127870

literature

• topic: Phylogeny
• Pubmed-ID: 14657106
• title: Soehngenia saccharolytica gen. nov., sp. nov. and Clostridium amygdalinum sp. nov., two novel anaerobic, benzaldehyde-converting bacteria.
• authors: Parshina SN, Kleerebezem R, Sanz JL, Lettinga G, Nozhevnikova AN, Kostrikina NA, Lysenko AM, Stams AJ
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.02668-0
• year: 2003
• mesh: Anaerobiosis, Benzaldehydes/*pharmacokinetics, Clostridium/*classification/growth & development/isolation & purification/metabolism, Culture Media, Gram-Positive Endospore-Forming Bacteria/*classification/growth & development/isolation & purification/metabolism, Molecular Sequence Data, *Phylogeny, Sewage/microbiology
• topic2: Enzymology

Reference