General

@ref: 5250

BacDive-ID: 2825

DSM-Number: 14207

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, psychrophilic, Gram-positive

description: Clostridium psychrophilum A-1/C-an/I is an anaerobe, spore-forming, psychrophilic bacterium that was isolated from microbial mat sample.

NCBI tax id

• NCBI tax id: 132926
• Matching level: species

strain history

• @ref: 5250
• history: <- H. Hippe, Leibniz-Institut DSMZ GmbH, Braunschweig, Germany; A-1/C-an/I <- H. Hippe {1999}

doi: 10.13145/bacdive2825.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacillota
• domain: Bacteria
• phylum: Bacillota
• class: Clostridia
• order: Eubacteriales
• family: Clostridiaceae
• genus: Clostridium
• species: Clostridium psychrophilum
• full scientific name: Clostridium psychrophilum Spring et al. 2003

@ref: 5250

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium psychrophilum

full scientific name: Clostridium psychrophilum Spring et al. 2003

strain designation: A-1/C-an/I, H12

type strain: yes

Morphology

cell morphology

• @ref: 69480
• gram stain: positive
• confidence: 99.999

colony morphology

• @ref: 5250
• incubation period: >14 days

Culture and growth conditions

culture medium

• @ref: 5250
• name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)
• growth: yes
• link: https://mediadive.dsmz.de/medium/339
• composition: Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

• @ref: 5250
• growth: positive
• type: growth
• temperature: 4
• range: psychrophilic

Physiology and metabolism

oxygen tolerance

spore formation

Isolation, sampling and environmental information

isolation

• @ref: 5250
• sample type: microbial mat sample
• geographic location: McMurdo Dry Valley Region, Lake Fryxell
• country: Antarctica
• origin.country: ATA
• continent: Australia and Oceania
• latitude: -77.6
• longitude: 163.1

isolation source categories

• Cat1: #Environmental
• Cat2: #Microbial community
• Cat3: #Microbial mat

Safety information

risk assessment

• @ref: 5250
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 5250
• description: Clostridium aff. estertheticum A-1/C-an/I partial 16S rRNA gene
• accession: AJ297443
• length: 1433
• database: ena
• NCBI tax ID: 132926

Genome sequences

GC content

• @ref: 5250
• GC-content: 31.8
• method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

External links

@ref: 5250

culture collection no.: DSM 14207, ATCC BAA 582

straininfo link

• @ref: 72349
• straininfo: 97654

literature

• topic: Phylogeny
• Pubmed-ID: 12892121
• title: Characterization of novel psychrophilic clostridia from an Antarctic microbial mat: description of Clostridium frigoris sp. nov., Clostridium lacusfryxellense sp. nov., Clostridium bowmanii sp. nov. and Clostridium psychrophilum sp. nov. and reclassification of Clostridium laramiense as Clostridium estertheticum subsp. laramiense subsp. nov.
• authors: Spring S, Merkhoffer B, Weiss N, Kroppenstedt RM, Hippe H, Stackebrandt E
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.02554-0
• year: 2003
• mesh: Antarctic Regions, Clostridium/*classification/genetics/isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fresh Water/microbiology, Genotype, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Terminology as Topic
• topic2: Genetics

Reference