Strain identifier

BacDive ID: 2821

Type strain: Yes

Species: Enterocloster bolteae

Strain history: CIP <- 2007, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10225 <- DSMZ <- ATCC <- S.M. Finegold: strain WAL 16351

NCBI tax ID(s): 208479 (species)

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General

@ref: 6024

BacDive-ID: 2821

DSM-Number: 15670

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Enterocloster bolteae JCM 12243 is an anaerobe, mesophilic, Gram-positive human pathogen that was isolated from stool specimens of children.

NCBI tax id

  • NCBI tax id: 208479
  • Matching level: species

strain history

@refhistory
6024<- ATCC; ATCC BAA-613 <- S. M. Finegold; WAL 16351
67770CCUG 46953 <-- Y. Song WAL 16351.
116836CIP <- 2007, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10225 <- DSMZ <- ATCC <- S.M. Finegold: strain WAL 16351

doi: 10.13145/bacdive2821.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Enterocloster
  • species: Enterocloster bolteae
  • full scientific name: Enterocloster bolteae (Song et al. 2003) Haas and Blanchard 2020

  • synonyms

    @refsynonym
    20215Lachnoclostridium bolteae
    20215Clostridium bolteae

@ref: 6024

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Enterocloster

species: Enterocloster bolteae

full scientific name: Enterocloster bolteae (Song et al. 2003) Haas and Blanchard 2020

type strain: yes

Morphology

cell morphology

  • @ref: 116836
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 57419
  • incubation period: 1 day

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6024PY + X MEDIUM (DSMZ Medium 104b)yeshttps://mediadive.dsmz.de/medium/104bName: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water
39709MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
116836CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
6024positivegrowth37mesophilic
39709positivegrowth37mesophilic
57419positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6024anaerobe
57419anaerobe
116836anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11683617632nitrate+reduction
11683616301nitrite-reduction

metabolite production

  • @ref: 116836
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
116836oxidase-
116836catalase-1.11.1.6
116836urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116836-+--------+-++-+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6024stool specimens of children
57419Human feces,child with late-onset autismLos AngelesUSAUSANorth America
67770Stool specimens of children with late-onset autism
116836Human, Feces

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Infection#Patient#Specimen
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Human#Child

taxonmaps

  • @ref: 69479
  • File name: preview.99_285.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_176;97_190;98_241;99_285&stattab=map
  • Last taxonomy: Enterocloster bolteae
  • 16S sequence: AJ508452
  • Sequence Identity:
  • Total samples: 91567
  • soil counts: 424
  • aquatic counts: 1298
  • animal counts: 89539
  • plant counts: 306

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
6024yes, in single cases1Risk group (German classification)
1168362Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Clostridium bolteae strain DSM 15670 16S ribosomal RNA gene, partial sequenceHM245933846ena208479
6024Clostridium boltei partial 16S rRNA gene, strain 16351AJ5084521498ena208479
67770Clostridium bolteae gene for 16S ribosomal RNA, partial sequence, strain: JCM 12243AB6492781390ena208479

Genome sequences

  • @ref: 67770
  • description: Enterocloster bolteae ATCC BAA-613
  • accession: GCA_000154365
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 411902

GC content

@refGC-contentmethod
602449.0sequence analysis
6777050.5high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes76.782no
flagellatedno64.312no
gram-positiveyes77.863no
anaerobicyes98.871yes
aerobicno97.594yes
halophileno89.55no
spore-formingyes72.774no
thermophileno97.812yes
glucose-utilyes92.422no
glucose-fermentyes74.363no

External links

@ref: 6024

culture collection no.: JCM 12243, DSM 15670, ATCC BAA 613, CCUG 46953, WAL 16351, CIP 109439, VTT E-052776, AIP 10225

straininfo link

  • @ref: 72345
  • straininfo: 110852

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12747414Clostridium bolteae sp. nov., isolated from human sources.Song Y, Liu C, Molitoris DR, Tomzynski TJ, Lawson PA, Collins MD, Finegold SMSyst Appl Microbiol10.1078/0723202033223373532003Clostridium/*classification/enzymology/genetics/isolation & purification, Culture Media, Esculin/metabolism, Feces/microbiology, Glucuronidase/biosynthesis, Humans, Microbial Sensitivity Tests, Phenotype, Phylogeny, RNA, Ribosomal, 16S/analysisEnzymology
Phylogeny23625266Fusicatenibacter saccharivorans gen. nov., sp. nov., isolated from human faeces.Takada T, Kurakawa T, Tsuji H, Nomoto KInt J Syst Evol Microbiol10.1099/ijs.0.045823-02013Adult, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Feces/*microbiology, Gram-Positive Asporogenous Rods/*classification/genetics/isolation & purification, Humans, Lysine/analysis, Middle Aged, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAPathogenicity
Pathogenicity35333269ROESY and (13)C NMR to distinguish between D- and L-rhamnose in the alpha-D-Manp-(1 --> 4)-beta-Rhap-(1 --> 3) repeating motif.Davidson J, Gauthier-Signore C, Bishop KP, Wicks C, Monteiro MA, Roy PN, Auzanneau FIOrg Biomol Chem10.1039/d2ob00131d2022*Autism Spectrum Disorder, Child, Disaccharides, Humans, Magnetic Resonance Spectroscopy, Mannose/chemistry, *Rhamnose/chemistry

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6024Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15670)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15670
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39709Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7183
57419Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 46953)https://www.ccug.se/strain?id=46953
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72345Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID110852.1StrainInfo: A central database for resolving microbial strain identifiers
116836Curators of the CIPCollection of Institut Pasteur (CIP 109439)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109439