Archive BacDiveID:10.13145/bacdive2816.20221219.7.1

Strain identifier

BacDive ID: 2816

Type strain:

Species: Clostridium thiosulfatireducens

Strain history: M.L. Fardeau, Université de Marseille, Marseille, France, strain Lup 21

NCBI tax ID(s): 150336 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 4906

BacDive-ID: 2816

DSM-Number: 13105

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Clostridium thiosulfatireducens DSM 13105 is an anaerobe, mesophilic bacterium that was isolated from granular sludge from an upflow anaerobic sludge blanket reactor.

NCBI tax id

NCBI tax id: 150336
Matching level: species

strain history

doi: 10.13145/bacdive2816.20221219.7.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/bacillota
domain: Bacteria
phylum: Bacillota
class: Clostridia
order: Eubacteriales
family: Clostridiaceae
genus: Clostridium
species: Clostridium thiosulfatireducens
full scientific name: Clostridium thiosulfatireducens Hernández-Eugenio et al. 2002

@ref: 4906

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium thiosulfatireducens

full scientific name: Clostridium thiosulfatireducens Hernández-Eugenio et al. 2002

type strain: yes

Morphology

cell morphology

@ref: 69480
gram stain: positive
confidence: 92.961

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
4906	ANAEROCOLUMNA MEDIUM (DSMZ Medium 860)	yes	https://mediadive.dsmz.de/medium/860	Name: ANAEROCOLUMNA MEDIUM (DSMZ Medium 860; with strain-specific modifications) Composition: Na2S2O3 x 5 H2O 2.49501 g/l Na2CO3 1.49701 g/l Yeast extract 0.998004 g/l NH4Cl 0.998004 g/l NaCl 0.598802 g/l L-Cysteine HCl x H2O 0.499002 g/l K2HPO4 0.299401 g/l KH2PO4 0.299401 g/l Na2S x 9 H2O 0.299401 g/l MgCl2 x 6 H2O 0.199601 g/l KCl 0.0998004 g/l CaCl2 x 2 H2O 0.0998004 g/l HCl 0.00374251 g/l FeCl2 x 4 H2O 0.00224551 g/l Sodium resazurin 0.000499002 g/l CoCl2 x 6 H2O 0.000284431 g/l MnCl2 x 4 H2O 0.000149701 g/l ZnCl2 0.00010479 g/l Na2MoO4 x 2 H2O 5.38922e-05 g/l NiCl2 x 6 H2O 3.59281e-05 g/l H3BO3 8.98204e-06 g/l CuCl2 x 2 H2O 2.99401e-06 g/l Distilled water
33017	MEDIUM 6 - Columbia agar with 10 % horse blood	yes		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)

culture temp

@ref	growth	type	temperature	range
4906	positive	growth	37	mesophilic
33017	positive	growth	37	mesophilic

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance	confidence
4906	anaerobe
69480	anaerobe	98.525

spore formation

@ref: 69480
spore formation: yes
confidence: 98.021

halophily

@ref: 69480
halophily level: non-halophilic
confidence: 99.163

Isolation, sampling and environmental information

isolation

@ref: 4906
sample type: granular sludge from an upflow anaerobic sludge blanket reactor
country: Mexico
origin.country: MEX
continent: North America

isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Bioreactor
#Engineered	#Waste	#Activated sludge
#Condition	#Anoxic (anaerobic)

taxonmaps

@ref: 69479
File name: preview.99_1064.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_614;97_717;98_836;99_1064&stattab=map
Last taxonomy: Clostridium
16S sequence: AF317650
Sequence Identity:
Total samples: 25309
soil counts: 12801
aquatic counts: 4474
animal counts: 5452
plant counts: 2582

Safety information

risk assessment

@ref: 4906
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Clostridium thiosulfatireducens gene for 16S rRNA, partial sequence, strain: DSM 13105	AB294141	1357	ena	150336
4906	Clostridium thiosulforeducens 16S ribosomal RNA gene, partial sequence	AF317650	1447	ena	150336

GC content

@ref: 4906
GC-content: 31.4
method: high performance liquid chromatography (HPLC)

External links

@ref: 4906

culture collection no.: DSM 13105, CIP 106908, Lup 21

straininfo link

@ref	passport
20218	http://www.straininfo.net/strains/302299
20218	http://www.straininfo.net/strains/302298

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	12361247	Clostridium thiosulfatireducens sp. nov., a proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor.	Hernandez-Eugenio G, Fardeau ML, Cayol JL, Patel BKC, Thomas P, Macarie H, Garcia JL, Ollivier B	Int J Syst Evol Microbiol	10.1099/00207713-52-5-1461	2002	Anaerobiosis, Bioreactors, Clostridium/*classification/isolation & purification/metabolism, DNA, Bacterial/genetics, Endopeptidases/metabolism, Fermentation, Genotype, Industrial Waste, Microscopy, Electron, Molecular Sequence Data, Oxidation-Reduction, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Sulfur/metabolism, Thiosulfates/metabolism	Metabolism
Phylogeny	19542123	Clostridium sulfidigenes sp. nov., a mesophilic, proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from pond sediment.	Sallam A, Steinbuchel A	Int J Syst Evol Microbiol	10.1099/ijs.0.004986-0	2009	Bacterial Proteins, Bacterial Typing Techniques, Base Composition, Clostridium/classification/genetics/isolation & purification/metabolism, DNA, Bacterial/analysis, Fresh Water/microbiology, Genes, rRNA, Genotype, Geologic Sediments/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Peptide Hydrolases/metabolism, Phenotype, Phylogeny, Plant Proteins/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Sulfur-Reducing Bacteria/classification/genetics/isolation & purification, Thiosulfates/metabolism	Metabolism
Phylogeny	25230767	Clostridium huakuii sp. nov., an anaerobic, acetogenic bacterium isolated from methanogenic consortia.	Ruan Z, Wang Y, Zhang C, Song J, Zhai Y, Zhuang Y, Wang H, Chen X, Li Y, Zhao B, Zhao B	Int J Syst Evol Microbiol	10.1099/ijs.0.062711-0	2014	Bacterial Typing Techniques, Base Composition, Biofuels, China, Clostridium/classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA	Genetics
Phylogeny	26419229	Clostridium punense sp. nov., an obligate anaerobe isolated from healthy human faeces.	Lanjekar VB, Marathe NP, Shouche YS, Ranade DR	Int J Syst Evol Microbiol	10.1099/ijsem.0.000644	2015	Bacterial Typing Techniques, Base Composition, Clostridium/classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/microbiology, Fermentation, Humans, India, Male, Middle Aged, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA	Pathogenicity

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
4906	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13105)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-13105
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
33017	Curators of the CIP		https://brclims.pasteur.fr/brcWeb/souche/detail/1/19091
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)