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Strain identifier

BacDive ID: 2816

Type strain: Yes

Species: Clostridium thiosulfatireducens

Strain history: M.L. Fardeau, Université de Marseille, Marseille, France, strain Lup 21

NCBI tax ID(s): 150336 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4906

BacDive-ID: 2816

DSM-Number: 13105

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Clostridium thiosulfatireducens DSM 13105 is an anaerobe, mesophilic bacterium that was isolated from granular sludge from an upflow anaerobic sludge blanket reactor.

NCBI tax id

  • NCBI tax id: 150336
  • Matching level: species

strain history

doi: 10.13145/bacdive2816.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium thiosulfatireducens
  • full scientific name: Clostridium thiosulfatireducens Hernández-Eugenio et al. 2002

@ref: 4906

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium thiosulfatireducens

full scientific name: Clostridium thiosulfatireducens Hernández-Eugenio et al. 2002

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 92.961

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4906ANAEROCOLUMNA MEDIUM (DSMZ Medium 860)yeshttps://mediadive.dsmz.de/medium/860Name: ANAEROCOLUMNA MEDIUM (DSMZ Medium 860; with strain-specific modifications) Composition: Na2S2O3 x 5 H2O 2.49501 g/l Na2CO3 1.49701 g/l Yeast extract 0.998004 g/l NH4Cl 0.998004 g/l NaCl 0.598802 g/l L-Cysteine HCl x H2O 0.499002 g/l K2HPO4 0.299401 g/l KH2PO4 0.299401 g/l Na2S x 9 H2O 0.299401 g/l MgCl2 x 6 H2O 0.199601 g/l KCl 0.0998004 g/l CaCl2 x 2 H2O 0.0998004 g/l HCl 0.00374251 g/l FeCl2 x 4 H2O 0.00224551 g/l Sodium resazurin 0.000499002 g/l CoCl2 x 6 H2O 0.000284431 g/l MnCl2 x 4 H2O 0.000149701 g/l ZnCl2 0.00010479 g/l Na2MoO4 x 2 H2O 5.38922e-05 g/l NiCl2 x 6 H2O 3.59281e-05 g/l H3BO3 8.98204e-06 g/l CuCl2 x 2 H2O 2.99401e-06 g/l Distilled water
33017MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)

culture temp

@refgrowthtypetemperaturerange
4906positivegrowth37mesophilic
33017positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
4906anaerobe
69480anaerobe98.525

spore formation

  • @ref: 69480
  • spore formation: yes
  • confidence: 98.021

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 99.163

Isolation, sampling and environmental information

isolation

  • @ref: 4906
  • sample type: granular sludge from an upflow anaerobic sludge blanket reactor
  • country: Mexico
  • origin.country: MEX
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Engineered#Bioreactor
#Engineered#Waste#Activated sludge
#Condition#Anoxic (anaerobic)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1064.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_614;97_717;98_836;99_1064&stattab=map
  • Last taxonomy: Clostridium
  • 16S sequence: AF317650
  • Sequence Identity:
  • Total samples: 25309
  • soil counts: 12801
  • aquatic counts: 4474
  • animal counts: 5452
  • plant counts: 2582

Safety information

risk assessment

  • @ref: 4906
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Clostridium thiosulfatireducens gene for 16S rRNA, partial sequence, strain: DSM 13105AB2941411357ena150336
4906Clostridium thiosulforeducens 16S ribosomal RNA gene, partial sequenceAF3176501447ena150336

GC content

  • @ref: 4906
  • GC-content: 31.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 4906

culture collection no.: DSM 13105, CIP 106908, Lup 21

straininfo link

@refpassport
20218http://www.straininfo.net/strains/302299
20218http://www.straininfo.net/strains/302298

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12361247Clostridium thiosulfatireducens sp. nov., a proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor.Hernandez-Eugenio G, Fardeau ML, Cayol JL, Patel BKC, Thomas P, Macarie H, Garcia JL, Ollivier BInt J Syst Evol Microbiol10.1099/00207713-52-5-14612002Anaerobiosis, Bioreactors, Clostridium/*classification/isolation & purification/metabolism, DNA, Bacterial/genetics, Endopeptidases/metabolism, Fermentation, Genotype, Industrial Waste, Microscopy, Electron, Molecular Sequence Data, Oxidation-Reduction, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Sulfur/metabolism, Thiosulfates/metabolismMetabolism
Phylogeny19542123Clostridium sulfidigenes sp. nov., a mesophilic, proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from pond sediment.Sallam A, Steinbuchel AInt J Syst Evol Microbiol10.1099/ijs.0.004986-02009Bacterial Proteins, Bacterial Typing Techniques, Base Composition, Clostridium/*classification/genetics/isolation & purification/metabolism, DNA, Bacterial/analysis, Fresh Water/*microbiology, Genes, rRNA, Genotype, Geologic Sediments/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Peptide Hydrolases/*metabolism, Phenotype, Phylogeny, Plant Proteins/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Sulfur-Reducing Bacteria/*classification/genetics/isolation & purification, Thiosulfates/*metabolismMetabolism
Phylogeny25230767Clostridium huakuii sp. nov., an anaerobic, acetogenic bacterium isolated from methanogenic consortia.Ruan Z, Wang Y, Zhang C, Song J, Zhai Y, Zhuang Y, Wang H, Chen X, Li Y, Zhao B, Zhao BInt J Syst Evol Microbiol10.1099/ijs.0.062711-02014Bacterial Typing Techniques, Base Composition, *Biofuels, China, Clostridium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny26419229Clostridium punense sp. nov., an obligate anaerobe isolated from healthy human faeces.Lanjekar VB, Marathe NP, Shouche YS, Ranade DRInt J Syst Evol Microbiol10.1099/ijsem.0.0006442015Bacterial Typing Techniques, Base Composition, Clostridium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Fermentation, Humans, India, Male, Middle Aged, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4906Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13105)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13105
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33017Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19091
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)