Strain identifier

BacDive ID: 2814

Type strain: No

Species: Clostridium tunisiense

Strain Designation: E1, E1T

Strain history: CIP <- 2002, M.L. Fardeau, IRD, Marseille, France: strain E1T

NCBI tax ID(s): 1233099 (strain), 219748 (species)

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General

@ref: 5757

BacDive-ID: 2814

DSM-Number: 15206

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Clostridium tunisiense E1 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from anaerobic mud of an olive mill wastewater basin.

NCBI tax id

NCBI tax idMatching level
219748species
1233099strain

strain history

@refhistory
5757<- M.-L. Fardeau, Universités de Provence et de la Méditerranée, ESIL, Marseille, France; E1 <- O. Ben Dhia Thabet {2001}
404772002, M.L. Fardeau, IRD, Marseille, France: strain E1T
115970CIP <- 2002, M.L. Fardeau, IRD, Marseille, France: strain E1T

doi: 10.13145/bacdive2814.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium tunisiense
  • full scientific name: Clostridium tunisiense Thabet et al. 2004

@ref: 5757

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium tunisiense

full scientific name: Clostridium tunisiense Thabet et al. 2004

strain designation: E1, E1T

type strain: no

Morphology

cell morphology

  • @ref: 115970
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 115970
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5757ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503)yeshttps://mediadive.dsmz.de/medium/503Name: ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503; with strain-specific modifications) Composition: Yeast extract 1.99402 g/l Trypticase peptone 1.99402 g/l Na2CO3 1.49551 g/l NaCl 0.997008 g/l KCl 0.498505 g/l MgCl2 x 6 H2O 0.398803 g/l Na2S x 9 H2O 0.299103 g/l NH4Cl 0.249252 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149552 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498505 g/l NaOH 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Nicotinic acid 0.000199402 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l Calcium pantothenate 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water
40477MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
115970CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5757positivegrowth37mesophilic
40477positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5757anaerobe
115970anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11597015824D-fructose+degradation
11597017634D-glucose-degradation
11597065327D-xylose-degradation
11597017057cellobiose-degradation
11597017716lactose-degradation
11597017306maltose+degradation
11597017814salicin-degradation
11597017992sucrose-degradation
1159704853esculin+hydrolysis
11597017632nitrate+reduction
11597016301nitrite+reduction
11597017632nitrate+respiration

antibiotic resistance

  • @ref: 115970
  • ChEBI: 6909
  • metabolite: metronidazole
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 115970
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
115970oxidase-
115970beta-galactosidase-3.2.1.23
115970gelatinase+
115970amylase-
115970DNase+
115970caseinase+3.4.21.50
115970catalase-1.11.1.6
115970tween esterase-
115970lecithinase+
115970lipase+
115970protease+
115970urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
115970--++----+-----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5757anaerobic mud of an olive mill wastewater basinSfaxTunisiaTUNAfrica
115970Environment, Anaerobic mud of an olive mill waste water basinTunisiaTUNAfrica2001

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial
#Engineered#Waste#Industrial wastewater
#Environmental#Terrestrial#Mud (Sludge)
#Condition#Anoxic (anaerobic)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4403.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_614;97_2668;98_3308;99_4403&stattab=map
  • Last taxonomy: Clostridium
  • 16S sequence: AY187622
  • Sequence Identity:
  • Total samples: 1716
  • soil counts: 602
  • aquatic counts: 562
  • animal counts: 456
  • plant counts: 96

Safety information

risk assessment

  • @ref: 115970
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5757
  • description: Clostridium tunisiense 16S ribosomal RNA gene, partial sequence
  • accession: AY187622
  • length: 1475
  • database: ena
  • NCBI tax ID: 1233099

External links

@ref: 5757

culture collection no.: DSM 15206, CIP 107666

literature

  • topic: Phylogeny
  • Pubmed-ID: 16701517
  • title: Clostridium tunisiense sp. nov., a new proteolytic, sulfur-reducing bacterium isolated from an olive mill wastewater contaminated by phosphogypse.
  • authors: Thabet OB, Fardeau ML, Joulian C, Thomas P, Hamdi M, Garcia JL, Ollivier B
  • journal: Anaerobe
  • DOI: 10.1016/j.anaerobe.2004.04.002
  • year: 2004

Reference

@idauthorscataloguedoi/urltitle
5757Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15206)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15206
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40477Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5126
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
115970Curators of the CIPCollection of Institut Pasteur (CIP 107666)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107666