Strain identifier

BacDive ID: 2813

Type strain: Yes

Species: Clostridium algidicarnis

Strain Designation: Dainty C

Strain history: <- NCIMB <- NCFB <- R. H. Dainty, Agricultural and Food Research Council, Institute of Food Research, Bristol, UK; Dainty C

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General

@ref: 5711

BacDive-ID: 2813

DSM-Number: 15099

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, psychrophilic, motile

description: Clostridium algidicarnis Dainty C is an anaerobe, spore-forming, psychrophilic bacterium that was isolated from vacuum-packed refrigerated pork.

NCBI tax id

NCBI tax idMatching level
37659species
1121295strain

strain history

  • @ref: 5711
  • history: <- NCIMB <- NCFB <- R. H. Dainty, Agricultural and Food Research Council, Institute of Food Research, Bristol, UK; Dainty C

doi: 10.13145/bacdive2813.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium algidicarnis
  • full scientific name: Clostridium algidicarnis Lawson et al. 1995

@ref: 5711

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium algidicarnis

full scientific name: Clostridium algidicarnis Lawson et al. 1995

strain designation: Dainty C

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes92.817
69480100positive

Culture and growth conditions

culture medium

  • @ref: 5711
  • name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/110
  • composition: Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water

culture temp

  • @ref: 5711
  • growth: positive
  • type: growth
  • temperature: 22
  • range: psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
5711anaerobe
69480anaerobe99.998

spore formation

@refspore formationconfidence
69481yes100
69480yes99.667

Isolation, sampling and environmental information

isolation

  • @ref: 5711
  • sample type: vacuum-packed refrigerated pork

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Bottled, canned, packed food
#Engineered#Food production#Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_2592.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_899;97_1048;98_2008;99_2592&stattab=map
  • Last taxonomy: Clostridium
  • 16S sequence: AF127023
  • Sequence Identity:
  • Total samples: 19709
  • soil counts: 4363
  • aquatic counts: 3822
  • animal counts: 10663
  • plant counts: 861

Safety information

risk assessment

  • @ref: 5711
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Clostridium algidicarnis 16S ribosomal RNA gene, partial sequenceAF1270231481ena37659
5711Clostridium algidicarnis 16S rRNA gene, strain NCFB 2931X776761511ena37659

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridium algidicarnis DSM 15099GCA_002934235scaffoldncbi1121295
66792Clostridium algidicarnis DSM 150991121295.3wgspatric1121295
66792Clostridium algidicarnis DSM 150992695420939draftimg1121295

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedyes61.628no
gram-positiveyes88.995no
anaerobicyes98.129yes
aerobicno95.674yes
halophileno80.529no
spore-formingyes97.198no
glucose-utilyes90.051no
thermophileno99.351yes
motileyes85.825no
glucose-fermentyes66.524no

External links

@ref: 5711

culture collection no.: DSM 15099, NCFB 2931, NCIMB 702931

straininfo link

  • @ref: 72339
  • straininfo: 62026

literature

  • topic: Phylogeny
  • Pubmed-ID: 9562877
  • title: Efficacy of heat and ethanol spore treatments for the isolation of psychrotrophic Clostridium spp. associated with the spoilage of chilled vacuum-packed meats.
  • authors: Broda DM, De Lacy KM, Bell RG
  • journal: Int J Food Microbiol
  • DOI: 10.1016/s0168-1605(97)00119-0
  • year: 1998
  • mesh: Animals, Cattle, Clostridium/drug effects/growth & development/*isolation & purification, Ethanol/*pharmacology, Food Microbiology, *Food Packaging, *Hot Temperature, Meat/*microbiology, Sheep, Spores, Bacterial/drug effects/growth & development/*isolation & purification, Time Factors
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5711Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15099)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15099
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72339Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID62026.1StrainInfo: A central database for resolving microbial strain identifiers