Strain identifier

BacDive ID: 2800

Type strain: Yes

Species: Clostridium acidisoli

Strain Designation: CK-74

Strain history: <- C. Matthies, University of Bayreuth, Department of Ecological Microbiology, Bayreuth, Germany; CK-74 <- <- C. H. Kuhner {1994}

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4763

BacDive-ID: 2800

DSM-Number: 12555

keywords: anaerobe, Bacteria, 16S sequence, genome sequence, mesophilic

description: Clostridium acidisoli CK-74 is an anaerobe, mesophilic bacterium that was isolated from acidic peat bog.

NCBI tax id

NCBI tax idMatching level
91624species
1121291strain

strain history: <- C. Matthies, University of Bayreuth, Department of Ecological Microbiology, Bayreuth, Germany; CK-74 <- <- C. H. Kuhner {1994}

doi: 10.13145/bacdive2800.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium acidisoli
  • full scientific name: Clostridium acidisoli Kuhner et al. 2000

@ref: 4763

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium acidisoli

full scientific name: Clostridium acidisoli Kuhner et al. 2000

strain designation: CK-74

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 4763
  • name: ANAEROBIC TRYPTIC SOY BROTH MEDIUM (DSMZ Medium 869)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/869
  • composition: Name: ANAEROBIC TRYPTIC SOY BROTH MEDIUM (DSMZ Medium 869) Composition: Trypticase soy broth 5.5 g/l D-Glucose 2.0 g/l Resazurin 0.0005 g/l Distilled water

culture temp

  • @ref: 4763
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 4763
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 4763
  • sample type: acidic peat bog
  • geographic location: Fichtelgebirge
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Wetland (Swamp)
#Condition#Acidic

Safety information

risk assessment

  • @ref: 4763
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4763
  • description: Clostridium acidisoli 16S rRNA gene, strain CK74
  • accession: AJ237756
  • length: 1498
  • database: ena
  • NCBI tax ID: 91624

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridium acidisoli DSM 12555 DSM 12555GCA_900176305contigncbi1121291
66792Clostridium acidisoli DSM 12555 strain DSM 125551121291.3wgspatric1121291
66792Clostridium acidisoli DSM 125552585428079draftimg1121291

GC content

  • @ref: 4763
  • GC-content: 30.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 4763

culture collection no.: DSM 12555, ATCC BAA 167

straininfo link

@refpassport
20218http://www.straininfo.net/strains/119001
20218http://www.straininfo.net/strains/119000

literature

  • topic: Phylogeny
  • Pubmed-ID: 10758899
  • title: Clostridium akagii sp. nov. and Clostridium acidisoli sp. nov.: acid-tolerant, N2-fixing clostridia isolated from acidic forest soil and litter.
  • authors: Kuhner CH, Matthies C, Acker G, Schmittroth M, Gossner AS, Drake HL
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-50-2-873
  • year: 2000
  • mesh: Anaerobiosis, Clostridium/*classification/isolation & purification/*physiology/ultrastructure, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Genes, rRNA, Hydrogen-Ion Concentration, Microscopy, Electron, Molecular Sequence Data, *Nitrogen Fixation, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Trees
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4763Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12555)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12555
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880