Strain identifier
BacDive ID: 2786
Type strain: 
Species: Clostridium acetireducens
Strain Designation: 30A
Strain history: <- J. C. Gottschal, Univ. Groningen, The Netherlands; 30A <- J. Örlygsson {1991}
NCBI tax ID(s): 1121290 (strain), 76489 (species)
version 8.1 (current version)
General
@ref: 4046
BacDive-ID: 2786
DSM-Number: 10703
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive
description: Clostridium acetireducens 30A is an anaerobe, spore-forming, mesophilic bacterium that was isolated from anaerobic bioreactor fed on waste from potato starch factory.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 76489 | species |
| 1121290 | strain |
strain history
- @ref: 4046
- history: <- J. C. Gottschal, Univ. Groningen, The Netherlands; 30A <- J. Örlygsson {1991}
doi: 10.13145/bacdive2786.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium acetireducens
- full scientific name: Clostridium acetireducens Örlygsson et al. 1996
@ref: 4046
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Clostridium
species: Clostridium acetireducens
full scientific name: Clostridium acetireducens Örlygsson et al. 1996
strain designation: 30A
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 100
Culture and growth conditions
culture medium
- @ref: 4046
- name: RCM MEDIUM (WITH CASAMINO ACIDS) (DSMZ Medium 634a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/634a
- composition: Name: RCM MEDIUM (with Casamino acids) (DSMZ Medium 634a) Composition: dehydrated RCM medium 38.0 g/l Casamino acids 15.0 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
- @ref: 4046
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 4046 | anaerobe | |
| 69480 | anaerobe | 99.998 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
Isolation, sampling and environmental information
isolation
- @ref: 4046
- sample type: anaerobic bioreactor fed on waste from potato starch factory
- geographic location: De Krim
- country: Netherlands
- origin.country: NLD
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Bioreactor | |
| #Engineered | #Waste | #Solid plant waste |
| #Condition | #Anoxic (anaerobic) |
Safety information
risk assessment
- @ref: 4046
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4046
- description: Clostridium sp. gene for 16S rRNA
- accession: X79862
- length: 1502
- database: ena
- NCBI tax ID: 76489
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Clostridium acetireducens DSM 10703 | GCA_001758365 | contig | ncbi | 1121290 |
| 66792 | Clostridium acetireducens DSM 10703 | 1121290.3 | wgs | patric | 1121290 |
| 66792 | Clostridium acetireducens DSM 10703 | 2687453717 | draft | img | 1121290 |
GC content
- @ref: 4046
- GC-content: 28.5
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | yes | 72.434 | no |
| flagellated | no | 74.332 | no |
| gram-positive | yes | 58.61 | no |
| anaerobic | yes | 99.998 | no |
| aerobic | no | 99.393 | no |
| halophile | no | 86.302 | no |
| spore-forming | yes | 93.703 | no |
| thermophile | no | 79.065 | yes |
| glucose-util | yes | 75.736 | no |
| glucose-ferment | yes | 60.115 | no |
External links
@ref: 4046
culture collection no.: DSM 10703
straininfo link
- @ref: 72313
- straininfo: 48861
literature
- topic: Genetics
- Pubmed-ID: 27979949
- title: Genome Sequence of the Poly-3-Hydroxybutyrate Producer Clostridium acetireducens DSM 10703.
- authors: Fluchter S, Poehlein A, Schiel-Bengelsdorf B, Daniel R, Durre P
- journal: Genome Announc
- DOI: 10.1128/genomeA.01399-16
- year: 2016
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 4046 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10703) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-10703 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 72313 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48861.1 | StrainInfo: A central database for resolving microbial strain identifiers |