Strain identifier

BacDive ID: 2781

Type strain: No

Species: Clostridium autoethanogenum

Strain Designation: JA1-1

Strain history: <- E.-J. Nyns, Catholic University of Louvain, Unit of Bioengineering, Louvain-la Neuve, Belgium; JA1-1

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 3787

BacDive-ID: 2781

DSM-Number: 10061

keywords: mesophilic, anaerobe, Bacteria, 16S sequence, genome sequence

description: Clostridium autoethanogenum JA1-1 is an anaerobe, mesophilic bacterium that was isolated from rabbit faeces.

NCBI tax id

NCBI tax idMatching level
84023species
1341692strain

strain history: <- E.-J. Nyns, Catholic University of Louvain, Unit of Bioengineering, Louvain-la Neuve, Belgium; JA1-1

doi: 10.13145/bacdive2781.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium autoethanogenum
  • full scientific name: Clostridium autoethanogenum Abrini et al. 1994

@ref: 3787

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium autoethanogenum

full scientific name: Clostridium autoethanogenum Abrini et al.

strain designation: JA1-1

type strain: no

Culture and growth conditions

culture medium

  • @ref: 3787
  • name: CLOSTRIDIUM LJUNGDAHLII MEDIUM (DSMZ Medium 879)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/879
  • composition: Name: CLOSTRIDIUM LJUNGDAHLII MEDIUM (DSMZ Medium 879) Composition: D-Fructose 5.0 g/l NaHCO3 1.0 g/l Yeast extract 1.0 g/l NH4Cl 1.0 g/l Na2S x 9 H2O 0.3 g/l L-Cysteine HCl x H2O 0.3 g/l KH2PO4 0.1 g/l KCl 0.1 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l CaCl2 x 2 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l Pyridoxine hydrochloride 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l (±)-alpha-Lipoic acid 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l Riboflavin 5e-05 g/l Thiamine HCl 5e-05 g/l Nicotinic acid 5e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp

  • @ref: 3787
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 3787
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 3787
  • sample type: rabbit faeces

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Leporidae (Rabbit/Hare)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Sequence information

16S sequences

  • @ref: 3787
  • description: Clostridium autoethanogenum 16S rRNA gene, partial, strain DSM 10061
  • accession: Y18178
  • length: 1460
  • database: ena
  • NCBI tax ID: 1341692

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridium autoethanogenum DSM 10061 DSM 10061GCA_001484725completencbi1341692
66792Clostridium autoethanogenum DSM 10061 DSM 10061GCA_000484505completencbi1341692
66792Clostridium autoethanogenum JA1-1GCA_002189005scaffoldncbi84023
66792Clostridium autoethanogenum DSM 10061 DSM 10061GCA_000427255contigncbi1341692
66792Clostridium autoethanogenum DSM 100611341692.11completepatric1341692
66792Clostridium autoethanogenum DSM 100611341692.10completepatric1341692
66792Clostridium autoethanogenum DSM 100611341692.4completepatric1341692
66792Clostridium autoethanogenum strain JA1-184023.6wgspatric84023
66792Clostridium autoethanogenum DSM 100612814123208completeimg1341692
66792Clostridium autoethanogenum DSM 100612554235459completeimg1341692
66792Clostridium autoethanogenum DSM 100612554235205draftimg1341692

GC content

@refGC-contentmethod
378725.9thermal denaturation, midpoint method (Tm)
378731.1sequence analysis

External links

@ref: 3787

culture collection no.: DSM 10061

straininfo link

  • @ref: 20218
  • passport: http://www.straininfo.net/strains/45146

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Metabolism23789275[Comparison of microorganisms fermenting syngas into ethanol].Song A, Feng X, Wang F, Xie H, Yang DSheng Wu Gong Cheng Xue Bao2013Bacteria/classification/*metabolism, Clostridium acetobutylicum/metabolism, Ethanol/isolation & purification/*metabolism, *Fermentation, Gases/*metabolism, Hydrogen/metabolismPhylogeny
Genetics23950130Genome Sequence of the Autotrophic Acetogen Clostridium autoethanogenum JA1-1 Strain DSM 10061, a Producer of Ethanol from Carbon Monoxide.Bruno-Barcena JM, Chinn MS, Grunden AMGenome Announc201310.1128/genomeA.00628-13
24655715Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia.Brown SD, Nagaraju S, Utturkar S, De Tissera S, Segovia S, Mitchell W, Land ML, Dassanayake A, Kopke MBiotechnol Biofuels201410.1186/1754-6834-7-40
Metabolism26331212Traits of selected Clostridium strains for syngas fermentation to ethanol.Martin ME, Richter H, Saha S, Angenent LTBiotechnol Bioeng2015Anaerobiosis, *Biofuels, Biotransformation, Carbon Monoxide/*metabolism, Clostridium/genetics/*metabolism, Ethanol/*metabolism, Fermentation, Hydrogen/*metabolism, Hydrogen-Ion Concentration, Metabolic Networks and Pathways/genetics10.1002/bit.25827
Genetics26692227Whole genome sequence and manual annotation of Clostridium autoethanogenum, an industrially relevant bacterium.Humphreys CM, McLean S, Schatschneider S, Millat T, Henstra AM, Annan FJ, Breitkopf R, Pander B, Piatek P, Rowe P, Wichlacz AT, Woods C, Norman R, Blom J, Goesman A, Hodgman C, Barrett D, Thomas NR, Winzer K, Minton NPBMC Genomics2015Clostridium/*genetics, Data Curation/methods, *Genome, Bacterial, High-Throughput Nucleotide Sequencing/methods, Molecular Sequence Annotation, Polymorphism, Single Nucleotide, Sequence Analysis, DNA/*methodsTranscriptome10.1186/s12864-015-2287-5

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3787Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10061)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10061
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880