Strain identifier
BacDive ID: 2773
Type strain:
Species: Thermoclostridium stercorarium
Strain history: <- ATCC <- NCIMB <- R. H. Madden
NCBI tax ID(s): 1121335 (strain), 1510 (species)
General
@ref: 3229
BacDive-ID: 2773
DSM-Number: 8532
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-positive
description: Thermoclostridium stercorarium DSM 8532 is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from compost heap.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1121335 | strain |
1510 | species |
strain history
- @ref: 3229
- history: <- ATCC <- NCIMB <- R. H. Madden
doi: 10.13145/bacdive2773.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Oscillospiraceae
- genus: Thermoclostridium
- species: Thermoclostridium stercorarium
- full scientific name: Thermoclostridium stercorarium (Madden 1983) Zhang et al. 2018
synonyms
- @ref: 20215
- synonym: Clostridium stercorarium
@ref: 3229
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Oscillospiraceae
genus: Thermoclostridium
species: Thermoclostridium stercorarium subsp. stercorarium
full scientific name: Thermoclostridium stercorarium subsp. stercorarium (Madden 1983) Zhang et al. 2018
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 99.999
Culture and growth conditions
culture medium
- @ref: 3229
- name: CLOSTRIDIUM (GS) MEDIUM (DSMZ Medium 255)
- growth: yes
- link: https://mediadive.dsmz.de/medium/255
- composition: Name: CLOSTRIDIUM (GS) MEDIUM (DSMZ Medium 255) Composition: Morpholinopropane sulfonic acid 9.98004 g/l Yeast extract 5.98802 g/l Cellobiose 4.99002 g/l Urea 1.99601 g/l L-Cysteine HCl x H2O 0.998004 g/l K2HPO4 x 3 H2O 0.998004 g/l KH2PO4 0.499002 g/l MgCl2 x 6 H2O 0.499002 g/l CaCl2 x 2 H2O 0.0499002 g/l FeSO4 x 7 H2O 0.0012475 g/l Sodium resazurin 0.000499002 g/l Distilled water
culture temp
@ref | growth | type | temperature | range | confidence |
---|---|---|---|---|---|
3229 | positive | growth | 60 | thermophilic | |
69480 | thermophilic | 98.977 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
3229 | anaerobe | |
69480 | anaerobe | 99.747 |
compound production
@ref | compound |
---|---|
3229 | xylanase |
3229 | exogluconase |
3229 | endoglucanase |
Isolation, sampling and environmental information
isolation
- @ref: 3229
- sample type: compost heap
- geographic location: Aberdeenshire
- country: United Kingdom
- origin.country: GBR
- continent: Europe
isolation source categories
- Cat1: #Engineered
- Cat2: #Biodegradation
- Cat3: #Composting
taxonmaps
- @ref: 69479
- File name: preview.99_1650.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_246;96_941;97_1095;98_1306;99_1650&stattab=map
- Last taxonomy: Thermoclostridium stercorarium subclade
- 16S sequence: AJ310082
- Sequence Identity:
- Total samples: 6000
- soil counts: 1258
- aquatic counts: 584
- animal counts: 3737
- plant counts: 421
Safety information
risk assessment
- @ref: 3229
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Clostridium stercorarium subsp. leptospartum 16S ribosomal RNA gene, partial sequence | AF266461 | 1518 | ena | 160385 |
20218 | Clostridium stercorarium (NCIMB 11754) 16S ribosomal RNA (16S rRNA) gene | L09174 | 1519 | ena | 1510 |
3229 | Clostridium stercorarium 16S rRNA gene, strain DSM 8532T | AJ310082 | 1500 | ena | 1510 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Thermoclostridium stercorarium subsp. stercorarium DSM 8532 | GCA_000331995 | complete | ncbi | 1121335 |
66792 | Thermoclostridium stercorarium subsp. stercorarium DSM 8532 | GCA_000349885 | complete | ncbi | 1121335 |
66792 | [Clostridium] stercorarium subsp. stercorarium DSM 8532 | 1121335.11 | complete | patric | 1121335 |
66792 | Clostridium stercorarium subsp. stercorarium DSM 8532 | 1121335.3 | complete | patric | 1121335 |
66792 | Thermoclostridium stercorarium stercorarium DSM 8532 | 2522125167 | complete | img | 1121335 |
66792 | Thermoclostridium stercorarium stercorarium DSM 8532 | 2540341047 | complete | img | 1121335 |
GC content
@ref | GC-content | method |
---|---|---|
3229 | 39 | thermal denaturation, midpoint method (Tm) |
3229 | 42.2 | sequence analysis |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 56 | no |
motile | yes | 71.555 | no |
flagellated | no | 81.107 | no |
gram-positive | yes | 69.234 | no |
anaerobic | yes | 98.96 | yes |
aerobic | no | 98.42 | no |
halophile | no | 93.968 | no |
spore-forming | yes | 86.501 | no |
thermophile | yes | 99.69 | no |
glucose-util | yes | 88.893 | no |
glucose-ferment | no | 51.094 | no |
External links
@ref: 3229
culture collection no.: DSM 8532, ATCC 35414, NCIMB 11754
straininfo link
- @ref: 72303
- straininfo: 41063
literature
- topic: Enzymology
- Pubmed-ID: 31685873
- title: Biochemical characterisation of four rhamnosidases from thermophilic bacteria of the genera Thermotoga, Caldicellulosiruptor and Thermoclostridium.
- authors: Baudrexl M, Schwarz WH, Zverlov VV, Liebl W
- journal: Sci Rep
- DOI: 10.1038/s41598-019-52251-0
- year: 2019
- mesh: Bacterial Proteins/chemistry/*metabolism, Caldicellulosiruptor, Cloning, Molecular, Clostridiales/*enzymology, Cobalt/chemistry/metabolism, Edetic Acid/chemistry/metabolism, Firmicutes/*enzymology, Flavanones/metabolism, Glycoside Hydrolases/chemistry/*metabolism, Hydrogen-Ion Concentration, Inhibitory Concentration 50, Kinetics, Recombinant Proteins/biosynthesis/chemistry/isolation & purification, Rhamnose/metabolism, Substrate Specificity, Temperature, Thermotoga maritima/*enzymology, Thermotoga neapolitana/*enzymology
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
3229 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8532) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-8532 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
72303 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID41063.1 | StrainInfo: A central database for resolving microbial strain identifiers |