Strain identifier

BacDive ID: 2769

Type strain: Yes

Species: Clostridium chauvoei

Strain Designation: 2585, 10123

Strain history: CIP <- 1994, J.P. Carlier, Inst. Pasteur, Paris, France: strain 10123 <- ATCC <- C.D. Stein: strain 2585

NCBI tax ID(s): 46867 (species)

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General

@ref: 3203

BacDive-ID: 2769

DSM-Number: 7528

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Clostridium chauvoei 2585 is an anaerobe, mesophilic, Gram-positive human pathogen that was isolated from bovine blackleg.

NCBI tax id

  • NCBI tax id: 46867
  • Matching level: species

strain history

@refhistory
3203<- ATCC <- C.D. Stein, 2585
118226CIP <- 1994, J.P. Carlier, Inst. Pasteur, Paris, France: strain 10123 <- ATCC <- C.D. Stein: strain 2585

doi: 10.13145/bacdive2769.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium chauvoei
  • full scientific name: Clostridium chauvoei (Arloing et al. 1887) Scott 1928 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium chauvoei

@ref: 3203

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium chauvoei

full scientific name: Clostridium chauvoei (Arloing et al. 1887) Scott 1928

strain designation: 2585, 10123

type strain: yes

Morphology

cell morphology

  • @ref: 118226
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3203RCM MEDIUM (DSMZ Medium 634)yeshttps://mediadive.dsmz.de/medium/634Name: RCM MEDIUM (DSMZ Medium 634) Composition: Sodium resazurin 0.0005 g/l dehydrated RCM medium
37173MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
118226CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20
118226CIP Medium 187yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=187

culture temp

@refgrowthtypetemperaturerange
3203positivegrowth37mesophilic
37173positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 3203
  • oxygen tolerance: anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11822617632nitrate-reduction
11822616301nitrite-reduction

metabolite production

  • @ref: 118226
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
118226oxidase-
118226catalase-1.11.1.6
118226urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118226-----------+--------

Isolation, sampling and environmental information

isolation

@refsample type
3203bovine blackleg
118226Animal, Bovine, black leg

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Mammals#Bovinae (Cow, Cattle)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4847.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_1253;97_2912;98_3629;99_4847&stattab=map
  • Last taxonomy: Clostridium
  • 16S sequence: U51843
  • Sequence Identity:
  • Total samples: 51283
  • soil counts: 5709
  • aquatic counts: 3525
  • animal counts: 41305
  • plant counts: 744

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
3203yesyes2Risk group (German classification)
1182262Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Clostridium chauvoei DNA, 16S-23S rRNA spacer regionAB040540286ena46867
20218Clostridium chauvoei DNA, 16S-23S rRNA spacer regionAB040541363ena46867
20218Clostridium chauvoei DNA, 16S-23S rRNA spacer regionAB040542441ena46867
20218Clostridium chauvoei genes for 16S rRNA, 16S-23S rRNA spacer region (rrn1), 23S rRNA, partial sequenceAB040712324ena46867
20218Clostridium chauvoei genes for 16S rRNA, tRNA-Ile, 23S rRNA, partial and complete sequenceAB040713401ena46867
20218Clostridium chauvoei genes for 16S rRNA, tRNA-Ala, tRNA-Ile, 23S rRNA, partial and complete sequenceAB040714479ena46867
3203Clostridium chauvoei 16S ribosomal RNA gene sequenceU518431507ena46867

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridium chauvoei DSM 7528GCA_002327185completencbi46867
66792Clostridium chauvoei strain 12S046746867.40completepatric46867
66792Clostridium chauvoei strain 12S046746867.42plasmidpatric46867
66792Clostridium chauvoei strain DSM 752846867.39completepatric46867
66792Clostridium chauvoei DSM 75282775507063completeimg46867

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes59.488no
gram-positiveyes91.666no
anaerobicyes98.368no
aerobicno97.274yes
halophileno76.105no
spore-formingyes94.846no
glucose-utilyes89.818no
thermophileno84.76yes
motileyes87.821no
glucose-fermentyes75.829no

External links

@ref: 3203

culture collection no.: DSM 7528, ATCC 10092, NCIMB 10665, CIP 104317

straininfo link

  • @ref: 72299
  • straininfo: 35178

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7518890Diversity in molecular mass of the common EDTA-soluble antigens of Clostridium chauvoei and Clostridium septicum.Hamaoka T, Terakado N, Nakamura SMicrobiol Immunol10.1111/j.1348-0421.1994.tb01756.x1994Animals, Antibodies, Monoclonal, Antigens, Bacterial/*chemistry/isolation & purification, Antigens, Surface/chemistry/isolation & purification, Clostridium/classification/*immunology, Cross Reactions, Edetic Acid, Epitopes/chemistry/isolation & purification, Fluorescent Antibody Technique, Immunoblotting, Mice, Molecular Weight, Solubility, Species SpecificityEnzymology
Enzymology16178463[Deoxyribonuclease activity detection in Clostridium chauvoei strains].Carloni GH, Bentancor LD, De Torres RARev Argent Microbiol2005Animals, Bacterial Proteins/*analysis, Cattle/microbiology, Cattle Diseases/microbiology, Clostridium/enzymology, Clostridium Infections/microbiology/veterinary, Clostridium chauvoei/*enzymology/isolation & purification, Clostridium perfringens/enzymology, Culture Media, Deoxyribonucleases/*analysis, Horses/blood, Serum, Species SpecificityPhylogeny
Enzymology17555280Extracellular proteins of Clostridium chauvoei are protective in a mouse model.Mattar MA, Cortinas TI, Stefanini AMActa Vet Hung10.1556/AVet.55.2007.2.12007Animals, Antibodies, Bacterial/*biosynthesis, Antigens, Bacterial, Bacterial Proteins/*immunology, Biological Assay, Blotting, Western/veterinary, Cell Cycle, Cells, Cultured, Clostridium chauvoei/growth & development/*immunology/isolation & purification, Dose-Response Relationship, Immunologic, Electrophoresis, Polyacrylamide Gel/veterinary, Enzyme-Linked Immunosorbent Assay/veterinary, Filtration/veterinary, Immunoglobulin G/blood, Mice, Molecular WeightGenetics
Genetics28720440First report of two complete Clostridium chauvoei genome sequences and detailed in silico genome analysis.Thomas P, Semmler T, Eichhorn I, Lubke-Becker A, Werckenthin C, Abdel-Glil MY, Wieler LH, Neubauer H, Seyboldt CInfect Genet Evol10.1016/j.meegid.2017.07.0182017Bacteriophages, Base Composition, Clostridium Infections/microbiology, Clostridium chauvoei/classification/*genetics/virology, Clustered Regularly Interspaced Short Palindromic Repeats, Computational Biology/methods, Drug Resistance, Bacterial, *Genome, Bacterial, *Genomics/methods, Molecular Sequence Annotation, Open Reading Frames, Phylogeny, Replication Origin, Sequence Analysis, DNA, Virulence FactorsPhylogeny
Enzymology29062200Cloning and sequence analysis of hyaluronoglucosaminidase (nagH) gene of Clostridium chauvoei.Dangi SK, Yadav PK, Tiwari A, Nagaleekar VKVet World10.14202/vetworld.2017.1104-11072017Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3203Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7528)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7528
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37173Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16213
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72299Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35178.1StrainInfo: A central database for resolving microbial strain identifiers
118226Curators of the CIPCollection of Institut Pasteur (CIP 104317)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104317