Strain identifier

BacDive ID: 2765

Type strain: Yes

Species: Anaerotignum neopropionicum

Strain Designation: X4

Strain history: <- Stefan Spring, DSM <- JP Touzel, Institut National de la Recherche Agronomique, Villeneuve-d'Ascq, France, X4 <- E Samain, Centre National de la Recherche Scientifique, Grenoble, France

NCBI tax ID(s): 1121327 (strain), 36847 (species)

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General

@ref: 1477

BacDive-ID: 2765

DSM-Number: 3847

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, chemoorganotroph, spore-forming, mesophilic, Gram-negative, motile, rod-shaped

description: Anaerotignum neopropionicum X4 is an anaerobe, chemoorganotroph, spore-forming bacterium that was isolated from anaerobic digester.

NCBI tax id

NCBI tax idMatching level
1121327strain
36847species

strain history

@refhistory
1477<- J. P. Touzel, Institut National de la Recherche Agronomique, Villeneuve-d'Ascq, France; X4 <- E. Samain, Centre National de la Recherche Scientifique, Grenoble, France {1981}
67771<- Stefan Spring, DSM <- JP Touzel, Institut National de la Recherche Agronomique, Villeneuve-d'Ascq, France, X4 <- E Samain, Centre National de la Recherche Scientifique, Grenoble, France

doi: 10.13145/bacdive2765.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Anaerotignum
  • species: Anaerotignum neopropionicum
  • full scientific name: Anaerotignum neopropionicum (Tholozan et al. 1995) Ueki et al. 2017

  • synonyms

    @refsynonym
    20215Tyzzerella neopropionica
    20215Clostridium neopropionicum

@ref: 1477

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Anaerotignum

species: Anaerotignum neopropionicum

full scientific name: Anaerotignum neopropionicum (Tholozan et al. 1995) Ueki et al. 2017

strain designation: X4

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43524negative2-5 µm0.5-0.6 µmrod-shapedyes
67771rod-shapedyes
67771negative

Culture and growth conditions

culture medium

  • @ref: 1477
  • name: ANAEROTIGNUM NEOPROPIONICUM MEDIUM (DSMZ Medium 318b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/318b
  • composition: Name: ANAEROTIGNUM NEOPROPIONICUM MEDIUM (DSMZ Medium 318b) Composition: KHCO3 3.94867 g/l NH4Cl 0.987167 g/l Yeast extract 0.493583 g/l Trypticase peptone 0.493583 g/l Na2S x 9 H2O 0.29615 g/l KH2PO4 0.29615 g/l L-Cysteine HCl x H2O 0.29615 g/l Nitrilotriacetic acid 0.126357 g/l MgCl2 x 6 H2O 0.0987167 g/l FeCl2 x 4 H2O 0.00987167 g/l NaCl 0.00987167 g/l NiCl2 x 6 H2O 0.000987167 g/l ZnCl2 0.000987167 g/l CaCl2 x 2 H2O 0.000987167 g/l MnCl2 x 4 H2O 0.000987167 g/l Sodium resazurin 0.000493583 g/l Na2WO4 x 2 H2O 0.000394867 g/l CoCl2 x 6 H2O 0.00029615 g/l Na2MoO4 x 2 H2O 0.00029615 g/l Na2SeO3 x 5 H2O 0.00029615 g/l CuCl2 0.000197433 g/l H3BO3 9.87167e-05 g/l Pyridoxine hydrochloride 9.87167e-05 g/l Riboflavin 4.93583e-05 g/l Thiamine HCl 4.93583e-05 g/l Nicotinic acid 4.93583e-05 g/l (DL)-alpha-Lipoic acid 4.93583e-05 g/l Calcium D-(+)-pantothenate 4.93583e-05 g/l p-Aminobenzoic acid 4.93583e-05 g/l Biotin 1.97433e-05 g/l Folic acid 1.97433e-05 g/l Vitamin B12 9.87167e-07 g/l Ethanol Distilled water

culture temp

@refgrowthtypetemperaturerange
1477positivegrowth30mesophilic
67771positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
1477anaerobe
43524obligate anaerobe
67771anaerobe

nutrition type

  • @ref: 43524
  • type: chemoorganotroph

spore formation

@refspore formationtype of spore
43524yes
67771yesspore

compound production

  • @ref: 1477
  • compound: propionate

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4352417234glucose-assimilation
4352416236ethanol+assimilation
4352416977L-alanine+assimilation
4352417115L-serine+assimilation
4352416857L-threonine+assimilation
4352418222xylose+assimilation
4352417191L-isoleucine+/-assimilation
4352415603L-leucine+/-assimilation
4352416414L-valine+/-assimilation

metabolite production

@refChebi-IDmetaboliteproduction
4352430089acetateyes
4352417968butyrateyes
4352448944isobutyrateyes
4352448942isovalerateyes
4352417272propionateyes

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
1477anaerobic digesterLilleFranceFRAEurope
43524anoxic digester sludge
67771From anoxic digestor sludgeLilleFranceFRAEurope

isolation source categories

Cat1Cat2Cat3
#Condition#Anoxic (anaerobic)
#Engineered#Biodegradation#Anaerobic digestor
#Engineered#Waste#Sewage sludge

Safety information

risk assessment

  • @ref: 1477
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 1477
  • description: Clostridium neopropionicum 16S rRNA gene, type strain DSM 3847T
  • accession: X76746
  • length: 1511
  • database: ena
  • NCBI tax ID: 36847

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerotignum neopropionicum X42651869904draftimg36847
66792Anaerotignum neopropionicum DSM 38472728369180draftimg36847

GC content

@refGC-contentmethod
147734.5
4352434.5high performance liquid chromatography (HPLC)
6777134.0-35.0

External links

@ref: 1477

culture collection no.: DSM 3847, KCTC 15564

straininfo link

  • @ref: 72295
  • straininfo: 47766

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics27081124Draft Genome Sequence of the Strict Anaerobe Clostridium neopropionicum X4 (DSM 3847T).Beck MH, Poehlein A, Bengelsdorf FR, Schiel-Bengelsdorf B, Daniel R, Durre PGenome Announc10.1128/genomeA.00209-162016
Phylogeny28905695Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum.Ueki A, Goto K, Ohtaki Y, Kaku N, Ueki KInt J Syst Evol Microbiol10.1099/ijsem.0.0022682017Animals, Bacterial Typing Techniques, Base Composition, Bioreactors/microbiology, Cattle, Clostridiales/*classification/genetics/isolation & purification, Clostridium/*classification, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Japan, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Waste Disposal, FluidTranscriptome
Phylogeny31680219Anaerotignum faecicola sp. nov., isolated from human faeces.Choi SH, Kim JS, Park JE, Lee KC, Eom MK, Oh BS, Yu SY, Kang SW, Han KI, Suh MK, Lee DH, Yoon H, Kim BY, Lee JH, Lee JH, Lee JS, Park SHJ Microbiol10.1007/s12275-019-9268-32019Bacterial Typing Techniques, Base Composition, Clostridiales/*classification/genetics/*isolation & purification/physiology, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Feces/*microbiology, Humans, Hydrogen-Ion Concentration, *Microbiota, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seoul, Sequence Analysis, DNA, TemperaturePathogenicity
Metabolism33990298Propionate Production from Carbon Monoxide by Synthetic Cocultures of Acetobacterium wieringae and Propionigenic Bacteria.Moreira JPC, Diender M, Arantes AL, Boeren S, Stams AJM, Alves MM, Alves JI, Sousa DZAppl Environ Microbiol10.1128/AEM.02839-202021Acetobacterium/drug effects/*metabolism, Bacterial Proteins/genetics/metabolism, Carbon Monoxide/*metabolism, Coculture Techniques, Deltaproteobacteria/drug effects/*metabolism, Fermentation, Propionates/*metabolism, Proteome/metabolism, Sodium Acetate/pharmacologyPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1477Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3847)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3847
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
43524Atsuko Ueki, Kazushi Goto, Yoshimi Ohtaki, Nobuo Kaku, Katsuji Ueki10.1099/ijsem.0.002268Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus AnaerotignumIJSEM 67: 4146-4153 201728905695
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
72295Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47766.1StrainInfo: A central database for resolving microbial strain identifiers