Strain identifier

BacDive ID: 275

Type strain: Yes

Species: Aeromonas salmonicida

Strain Designation: 20

Strain history: CIP <- 1988, NCIB <- I.W. Smith: strain 20 <- Public Hlth. Lab., Carmarthen, Wales, UK

NCBI tax ID(s): 645 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8224

BacDive-ID: 275

DSM-Number: 19634

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped, animal pathogen

description: Aeromonas salmonicida 20 is a facultative anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from salmon.

NCBI tax id

  • NCBI tax id: 645
  • Matching level: species

strain history

@refhistory
8224<- NCIMB <- I. W. Smith, Marine Laboratory <- Public Health Laboratory, Carmarthen, Wales
67770ATCC 33658 <-- NCMB 1102 <-- I. W. Smith <-- Public Health Lab., Carmarthen, Wales, UK.
122396CIP <- 1988, NCIB <- I.W. Smith: strain 20 <- Public Hlth. Lab., Carmarthen, Wales, UK

doi: 10.13145/bacdive275.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Aeromonas
  • species: Aeromonas salmonicida
  • full scientific name: Aeromonas salmonicida (Lehmann and Neumann 1896) Griffin et al. 1953 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacterium salmonicida

@ref: 8224

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Aeromonadales

family: Aeromonadaceae

genus: Aeromonas

species: Aeromonas salmonicida subsp. salmonicida

full scientific name: Aeromonas salmonicida subsp. salmonicida (Lehmann and Neumann 1896) Schubert 1967

strain designation: 20

type strain: yes

Morphology

cell morphology

  • @ref: 122396
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8224REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
41734MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122396CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
122396CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
8224positivegrowth28mesophilic
41734positivegrowth30mesophilic
67770positivegrowth20psychrophilic
122396positivegrowth22-30
122396nogrowth5psychrophilic
122396nogrowth10psychrophilic
122396nogrowth37mesophilic
122396nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122396
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
122396NaClpositivegrowth0-4 %
122396NaClnogrowth6 %
122396NaClnogrowth8 %
122396NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
12239617632nitrate+reduction
12239616301nitrite-reduction
122396132112sodium thiosulfate-builds gas from

antibiotic resistance

  • @ref: 122396
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
12239635581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
6836815688acetoin-
6836835581indole-
12239615688acetoin-
12239617234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68368cytochrome oxidase+1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368beta-galactosidase-3.2.1.23
122396oxidase+
122396beta-galactosidase+3.2.1.23
122396alcohol dehydrogenase-1.1.1.1
122396amylase+
122396catalase-1.11.1.6
122396tween esterase+
122396lysine decarboxylase+4.1.1.18
122396ornithine decarboxylase-4.1.1.17
122396urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122396--------------------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
8224-+--------+++-------+
8224----------+++-------+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
8224+-++-++++--+++++-+--+
8224+-++-++++--+++++-+--+
8224+-++-++++--+++++-+--+
8224+-++-++++--+++++-+--+
8224+-+--++++--++++--+--+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122396---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinenthost species
8224salmonRiver CletterUnited KingdomGBREurope
67770Salmon (Salmo salar)Salmo salar
122396Animal, Fish, salmonCletter RiverUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Host#Fishes
#Host#Fishes#Salmonidae

taxonmaps

  • @ref: 69479
  • File name: preview.99_485.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_11;96_15;97_15;98_18;99_485&stattab=map
  • Last taxonomy: Aeromonas
  • 16S sequence: X60405
  • Sequence Identity:
  • Total samples: 313
  • soil counts: 16
  • aquatic counts: 156
  • animal counts: 131
  • plant counts: 10

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
8224yes1Risk group (German classification)
1223961Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Aeromonas salmonicida subsp. salmonicida strain CECT 894T 16S ribosomal RNA gene, complete sequenceAY9877511510ena29491
20218Aeromonas salmonicida clone 2 16S ribosomal RNA gene, partial sequence; and 16S-23S ribosomal RNA intergenic spacer, complete sequenceEF373989536ena645
20218Aeromonas salmonicida clone 3 16S ribosomal RNA gene, partial sequence; and 16S-23S ribosomal RNA intergenic spacer, complete sequenceEF373990518ena645
20218Aeromonas salmonicida strain ATCC 33658 clone 4 16S-23S ribosomal RNA intergenic spacer, complete sequenceEF373991517ena645
20218Aeromonas salmonicida strain ATCC 33658 clone 1 16S-23S ribosomal RNA intergenic spacer, complete sequenceEF373992513ena645
20218Aeromonas salmonicida subsp. salmonicida strain CIP 103209 16S ribosomal RNA gene, partial sequenceJX014449151ena29491
20218Aeromonas salmonicida subsp. salmonicida strain ATCC 33658 16S ribosomal RNA gene, complete sequenceKC2447771287ena29491
20218Aeromonas salmonicida subsp. salmonicida 16S rRNA gene, strain NCIMB 1102X604051503ena29491
20218A.salmonicida (ssp. NCMB 1102) gene for 16S ribosomal RNAX718361283ena29491
8224A.salmonicida salmonicida (ATCC 33658T) gene for 16S ribosomal RNAX746811460ena29491
67770Aeromonas salmonicida subsp. salmonicida gene for 16S rRNA, strain: JCM 7874AB0275411467ena29491

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aeromonas salmonicida strain NCTC12959645.114wgspatric645
66792Aeromonas salmonicida subsp. salmonicida CIP 10320929491.11wgspatric29491
66792Aeromonas salmonicida subsp. salmonicida strain ATCC 3365829491.22wgspatric29491
66792Aeromonas salmonicida salmonicida ATCC 336582744054639draftimg29491
66792Aeromonas salmonicida salmonicida CIP 1032092648501469draftimg29491
67770Aeromonas salmonicida subsp. salmonicida CIP 103209GCA_000820065scaffoldncbi29491
67770Aeromonas salmonicida subsp. salmonicida ATCC 33658GCA_001643305contigncbi29491
67770Aeromonas salmonicida NCTC12959GCA_900445115contigncbi645

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.852no
anaerobicno96.086no
halophileno89.435no
spore-formingno95.014no
glucose-utilyes88.328no
thermophileno99.57yes
flagellatedyes92.588no
aerobicyes85.431no
motileyes89.77no
glucose-fermentyes88.253yes

External links

@ref: 8224

culture collection no.: DSM 19634, ATCC 33658, CAIM 346, CECT 894, CIP 103209, JCM 7874, LMG 3780, NCIMB 1102, NCTC 12959, GTC 02802, IMET 11051, KCTC 12266, DSM 3241

straininfo link

  • @ref: 69957
  • straininfo: 388

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity12958263Antimicrobial susceptibility testing of aquatic bacteria: quality control disk diffusion ranges for Escherichia coli ATCC 25922 and Aeromonas salmonicida subsp. salmonicida ATCC 33658 at 22 and 28 degrees C.Miller RA, Walker RD, Baya A, Clemens K, Coles M, Hawke JP, Henricson BE, Hsu HM, Mathers JJ, Oaks JL, Papapetropoulou M, Reimschuessel RJ Clin Microbiol10.1128/JCM.41.9.4318-4323.20032003Aeromonas/*drug effects, Animals, Diffusion, Escherichia coli/*drug effects, Microbial Sensitivity Tests/*standards, Quality Control, Temperature, *Water Microbiology
14735937Association of Type III secretion genes with virulence of Aeromonas salmonicida subsp. salmonicida.Burr SE, Wahli T, Segner H, Pugovkin D, Frey JDis Aquat Organ10.3354/dao0571672003Aeromonas/*genetics/*pathogenicity, Animals, Blotting, Southern, DNA Primers, Fish Diseases/*microbiology, Gram-Negative Bacterial Infections/*mortality, Oncorhynchus mykiss, Virulence, Virulence Factors/*genetics
Pathogenicity15997819Standardization of a broth microdilution susceptibility testing method to determine minimum inhibitory concentrations of aquatic bacteria.Miller RA, Walker RD, Carson J, Coles M, Coyne R, Dalsgaard I, Gieseker C, Hsu HM, Mathers JJ, Papapetropoulou M, Petty B, Teitzel C, Reimschuessel RDis Aquat Organ10.3354/dao0642112005Aeromonas salmonicida/*drug effects, Anti-Bacterial Agents/*toxicity, Culture Media/chemistry, Escherichia coli/*drug effects, Microbial Sensitivity Tests/*methods/*standards, Quality Control, Reproducibility of Results, TemperatureCultivation
Enzymology19447190Isolation and cDNA cloning of an antibacterial L-amino acid oxidase from the skin mucus of the great sculpin Myoxocephalus polyacanthocephalus.Nagashima Y, Tsukamoto C, Kitani Y, Ishizaki S, Nagai H, Yanagimoto TComp Biochem Physiol B Biochem Mol Biol10.1016/j.cbpb.2009.05.0062009Amino Acid Sequence, Animals, Anti-Bacterial Agents/*isolation & purification/metabolism/pharmacology, Bacteria/drug effects, Cloning, Molecular, DNA, Complementary/*genetics, Fishes/*genetics, L-Amino Acid Oxidase/*genetics/*isolation & purification/metabolism/pharmacology, Molecular Sequence Data, Mucus/*enzymology, Sequence Alignment, *SkinPhylogeny
Phylogeny20298453First description of atypical furunculosis in freshwater farmed Atlantic salmon, Salmo salar L., in Chile.Godoy M, Gherardelli V, Heisinger A, Fernandez J, Olmos P, Ovalle L, Ilardi P, Avendano-Herrera RJ Fish Dis10.1111/j.1365-2761.2010.01142.x2010Aeromonas salmonicida/*genetics/isolation & purification, Animals, Chile, Fish Diseases/*microbiology/*pathology, *Fresh Water, Furunculosis/microbiology/pathology/*veterinary, Genes, Bacterial/genetics, Molecular Sequence Data, Phenotype, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Salmo salarGenetics
Pathogenicity23324417Quality control ranges for testing broth microdilution susceptibility of Flavobacterium columnare and F. psychrophilum to nine antimicrobials.Gieseker CM, Mayer TD, Crosby TC, Carson J, Dalsgaard I, Darwish AM, Gaunt PS, Gao DX, Hsu HM, Lin TL, Oaks JL, Pyecroft M, Teitzel C, Somsiri T, Wu CCDis Aquat Organ10.3354/dao025272012Animals, Anti-Bacterial Agents/*pharmacology, *Drug Resistance, Bacterial, Flavobacterium/*drug effects, Microbial Sensitivity Tests/*methods, Reproducibility of Results, Sensitivity and Specificity
Pathogenicity25667335Marine Lactobacillus pentosus H16 protects Artemia franciscana from Vibrio alginolyticus pathogenic effects.Garces ME, Sequeiros C, Olivera NLDis Aquat Organ10.3354/dao028152015Animals, Antibiosis/physiology, Artemia/*microbiology, Host-Pathogen Interactions, Lactobacillus/*physiology, Vibrio alginolyticus/*physiology
25773973Improving survival and storage stability of bacteria recalcitrant to freeze-drying: a coordinated study by European culture collections.Peiren J, Buyse J, De Vos P, Lang E, Clermont D, Hamon S, Begaud E, Bizet C, Pascual J, Ruvira MA, Macian MC, Arahal DRAppl Microbiol Biotechnol10.1007/s00253-015-6476-62015Bacterial Physiological Phenomena/*radiation effects, Biological Specimen Banks, Freeze Drying/*methods, Microbial Viability/*radiation effects, Preservation, Biological/*methods
Cultivation26949840Development of Similar Broth Microdilution Methods to Determine the Antimicrobial Susceptibility of Flavobacterium columnare and F. psychrophilum.Gieseker CM, Crosby TC, Mayer TD, Bodeis SM, Stine CBJ Aquat Anim Health10.1080/08997659.2015.11058782016Animals, Anti-Bacterial Agents/*pharmacology, Bacteriological Techniques/*methods, Culture Media, Dose-Response Relationship, Drug, *Drug Resistance, Bacterial, Flavobacterium/*drug effects, Horses/blood, Microbial Sensitivity TestsPathogenicity
Genetics28982999Draft Genome Sequence of the Type Strain Aeromonas salmonicida subsp. salmonicida ATCC 33658.Valderrama K, Soto-Davila M, Santander JGenome Announc10.1128/genomeA.01064-172017
29259599Epidemiology of Danish Aeromonas salmonicida subsp. salmonicida in Fish Farms Using Whole Genome Sequencing.Bartkova S, Leekitcharoenphon P, Aarestrup FM, Dalsgaard IFront Microbiol10.3389/fmicb.2017.024112017

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8224Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19634)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19634
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41734Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14982
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69957Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID388.1StrainInfo: A central database for resolving microbial strain identifiers
122396Curators of the CIPCollection of Institut Pasteur (CIP 103209)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103209