Strain identifier

BacDive ID: 2741

Type strain: Yes

Species: Peptoclostridium litorale

Strain Designation: W6

Strain history: <- C. Fendrich, W6

NCBI tax ID(s): 1121324 (strain), 1557 (species)

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@ref: 2132

BacDive-ID: 2741

DSM-Number: 5388

keywords: motile, Gram-negative, mesophilic, spore-forming, anaerobe, Bacteria, 16S sequence, genome sequence, curved-shaped, colony-forming

description: Peptoclostridium litorale W6 is an anaerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from anoxic marine sediment.

NCBI tax id

NCBI tax idMatching level

strain history: <- C. Fendrich, W6

doi: 10.13145/bacdive2741.20220920.7

Name and taxonomic classification


  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptostreptococcaceae
  • genus: Peptoclostridium
  • species: Peptoclostridium litorale
  • full scientific name: Peptoclostridium litorale (Fendrich et al. 1991) Galperin et al. 2016
  • synonyms

    • @ref: 20215
    • synonym: Clostridium litorale

@ref: 2132

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Peptostreptococcaceae

genus: Peptoclostridium

species: Peptoclostridium litorale

full scientific name: Peptoclostridium litorale (Fendrich et al. 1991) Galperin et al. 2016

strain designation: W6

type strain: yes


cell morphology

  • @ref: 25060
  • gram stain: negative
  • cell length: 2.0-8.0 µm
  • cell width: 0.5-1.5 µm
  • cell shape: curved-shaped
  • motility: yes

colony morphology

  • @ref: 25060
  • colony color: brown
  • colony shape: circular

Culture and growth conditions

culture medium

  • @ref: 2132
  • growth: yes
  • link:
  • composition: Name: PEPTOCLOSTRIDIUM MEDIUM (BETAINE) (DSMZ Medium 487) Composition: NaCl 10.0 g/l Betaine x H2O 6.0 g/l NaHCO3 5.0 g/l L-Alanine 2.2 g/l NH4Cl 1.0 g/l Yeast extract 1.0 g/l MgSO4 x 7 H2O 0.5 g/l K2HPO4 0.36 g/l Na2S x 9 H2O 0.3 g/l KH2PO4 0.23 g/l CaCl2 x 2 H2O 0.03 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l NaOH 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l Pyridoxine hydrochloride 0.0001 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l (±)-alpha-Lipoic acid 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l Nicotinic acid 5e-05 g/l Riboflavin 5e-05 g/l Thiamine HCl 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l H3BO3 6e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp


culture pH


Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
25060obligate anaerobe

spore formation

  • @ref: 25060
  • spore description: ovoid,subterminal,1.5-2.0 µm in diameter
  • type of spore: spore
  • spore formation: yes


  • @ref: 25060
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 6 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested


25060cytochrome oxidase-

Isolation, sampling and environmental information


  • @ref: 2132
  • sample type: anoxic marine sediment
  • geographic location: North Sea coast, Jadebusen
  • country: Germany
  • DEU
  • continent: Europe

isolation source categories

#Condition#Anoxic (anaerobic)

Safety information

risk assessment

  • @ref: 2132
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 2132
  • description: Clostridium litorale 16S rRNA gene, strain DSM 5388
  • accession: X77845
  • length: 1507
  • database: ena
  • NCBI tax ID: 1557

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Peptoclostridium litorale DSM 5388 W6GCA_000699585contigncbi1121324
66792Peptoclostridium litorale DSM 5388 DSM 5388GCA_900141635scaffoldncbi1121324
66792[Clostridium] litorale DSM 53881121324.3wgspatric1121324
66792Peptoclostridium litorale DSM 53881121324.4wgspatric1121324
66792Peptoclostridium litorale DSM 53882585428090draftimg1121324
66792Peptoclostridium litorale W62561511002draftimg1121324

GC content

213226.1thermal denaturation, midpoint method (Tm)
2506041.3genome sequence analysis
213241.3sequence analysis

External links

@ref: 2132

culture collection no.: DSM 5388, ATCC 49638

straininfo link



  • Pubmed-ID: 25081264
  • title: First Insights into the Genome of the Amino Acid-Metabolizing Bacterium Clostridium litorale DSM 5388.
  • authors: Poehlein A, Alghaithi HS, Chandran L, Chibani CM, Davydova E, Dhamotharan K, Ge W, Gutierrez-Gutierrez DA, Jagirdar A, Khonsari B, Nair KP, Daniel R
  • journal: Genome Announc
  • DOI: 10.1128/genomeA.00754-14
  • year: 2014


2132Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5388)
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
25060M. Y. B. Galperin, V.,Tolstoy, I.,Yutin, N.10.1099/ijsem.0.001548Phylogenomic analysis of the family Peptostreptococcaceae (Clostridium cluster XI) and proposal for reclassification of Clostridium litorale (Fendrich et al. 1991) and Eubacterium acidaminophilum (Zindel et al. 1989) as Peptoclostridium litorale gen. nov. comb. nov. and Peptoclostridium acidaminophilum comb. novIJSEM 66: 5506-5513 201627902180
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880