Strain identifier

BacDive ID: 274

Type strain: Yes

Species: Aeromonas salmonicida subsp. masoucida

Strain Designation: T. Kimura 1-a-1, I-a-1

Strain history: CIP <- 1988, NCIB <- 1975, T. Kimura: strain I-a-1

NCBI tax ID(s): 1216980 (strain), 197700 (subspecies)

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General

@ref: 15922

BacDive-ID: 274

DSM-Number: 21760

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, Gram-negative, animal pathogen

description: Aeromonas salmonicida subsp. masoucida T. Kimura 1-a-1 is a facultative anaerobe, Gram-negative animal pathogen that was isolated from Heart blood of salmonid .

NCBI tax id

NCBI tax idMatching level
197700subspecies
1216980strain

strain history

@refhistory
15922<- CCM <- T. Kimura, Laboratory of Microbiology, Faculty of Fisheries, Hokkaido Uuiversity, Japan; 1-a-1
67770ATCC 27013 <-- R. H. W. Schubert 1-a-1 <-- T. Kimura.
122392CIP <- 1988, NCIB <- 1975, T. Kimura: strain I-a-1

doi: 10.13145/bacdive274.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Aeromonas
  • species: Aeromonas salmonicida subsp. masoucida
  • full scientific name: Aeromonas salmonicida subsp. masoucida Kimura 1969 (Approved Lists 1980)

@ref: 15922

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Aeromonadales

family: Aeromonadaceae

genus: Aeromonas

species: Aeromonas salmonicida subsp. masoucida

full scientific name: Aeromonas salmonicida subsp. masoucida Kimura 1969

strain designation: T. Kimura 1-a-1, I-a-1

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
122392negativerod-shapedno
69480negative99.994
69480yes90.503

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15922TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
38121MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
15922COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
122392CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
122392CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
15922positivegrowth28
38121positivegrowth22
15922positivegrowth30
67770positivegrowth25
122392positivegrowth5-37
122392nogrowth41

Physiology and metabolism

oxygen tolerance

  • @ref: 122392
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 90.965

halophily

@refsaltgrowthtested relationconcentration
122392NaClpositivegrowth0-4 %
122392NaClnogrowth6 %
122392NaClnogrowth8 %
122392NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68368L-arabinose-fermentation30849
68368amygdalin-fermentation27613
68368melibiose-fermentation28053
68368sucrose+fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol+fermentation16899
68368D-glucose-fermentation17634
68368gelatin+hydrolysis5291
68368tryptophan+energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
122392mannitol+fermentation29864
122392citrate-carbon source16947
122392glucose+fermentation17234
122392lactose-fermentation17716
122392nitrate+reduction17632
122392nitrite-reduction16301
122392malonate-assimilation15792
122392sodium thiosulfate-builds gas from132112
122392glucose+degradation17234

antibiotic resistance

  • @ref: 122392
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno
12239235581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole+
12239215688acetoin+
12239217234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase+1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368beta-galactosidase+3.2.1.23
122392oxidase+
122392beta-galactosidase+3.2.1.23
122392alcohol dehydrogenase+1.1.1.1
122392gelatinase+
122392amylase+
122392DNase+
122392caseinase+3.4.21.50
122392catalase+1.11.1.6
122392tween esterase+
122392gamma-glutamyltransferase+2.3.2.2
122392lecithinase-
122392lipase-
122392lysine decarboxylase-4.1.1.18
122392ornithine decarboxylase-4.1.1.17
122392phenylalanine ammonia-lyase+4.3.1.24
122392protease+
122392tryptophan deaminase-
122392urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122392-+++-+-++-++-+-+-+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
15922+-------+-+-+---+---+
15922++------+-+-+---+---+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122392+--++----++++----+---+-+++++--++---++---------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122392+++++--+-++----+-+---------------+---------------+----------+------------------++-+------+---------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
15922Heart blood of salmonid (Oncorhynchus masou)Oncorhynchus masouhatcheries in HokkaidoJapanJPNAsia
67770Masou (Oncorhynchus sp.)Oncorhynchus sp.
122392Animal, Fish blood, Oncorhynchus masou

isolation source categories

Cat1Cat2Cat3
#Host#Fishes#Salmonidae
#Host Body-Site#Organ#Heart
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_485.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_11;96_15;97_15;98_18;99_485&stattab=map
  • Last taxonomy: Aeromonas
  • 16S sequence: X74680
  • Sequence Identity:
  • Total samples: 313
  • soil counts: 16
  • aquatic counts: 156
  • animal counts: 131
  • plant counts: 10

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
15922yes1Risk group (German classification)
1223921Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Aeromonas salmonicida 16S rRNAD11175130nuccore645
20218Aeromonas salmonicida 16S rRNAD11224155nuccore645
20218Aeromonas salmonicida 16s rRNAD11273159nuccore645
15922A.salmonicida masoucida (ATCC 27013T) gene for 16S ribosomal RNAX746801460nuccore197700
67770Aeromonas salmonicida subsp. masoucida gene for 16S rRNA, strain: JCM 7873AB0275421467nuccore197700
124043Aeromonas salmonicida subsp. masoucida gene for 16S rRNA, partial sequence, strain: NBRC 13784.AB6805141470nuccore197700
124043Aeromonas salmonicida subsp. masoucida strain NBRC 13784 16S ribosomal RNA gene, partial sequence.MN526992493nuccore197700

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aeromonas salmonicida strain CIP 103210645.180wgspatric645
66792Aeromonas salmonicida subsp. masoucida NBRC 137841216980.4wgspatric1216980
66792Aeromonas salmonicida masoucida NBRC 137842576861489draftimg1216980
67770Aeromonas salmonicida subsp. masoucida NBRC 13784GCA_000647955contigncbi1216980
67770Aeromonas salmonicida CIP 103210GCA_006246135scaffoldncbi645

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingno99.994no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no95.221yes
69480spore-formingspore-formingAbility to form endo- or exosporesno90.965no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no71.992yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno100no
69480flagellatedmotile2+Ability to perform flagellated movementyes90.503no

External links

@ref: 15922

culture collection no.: DSM 21760, ATCC 27013, CCM 4124, CECT 896, CIP 103210, IFO 13784, JCM 8191, LMG 3782, NCIMB 2020, JCM 7873, JCM 8234, NBRC 13784

straininfo link

  • @ref: 69956
  • straininfo: 384

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15922Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21760)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21760
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38121Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14983
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69956Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID384.1StrainInfo: A central database for resolving microbial strain identifiers
122392Curators of the CIPCollection of Institut Pasteur (CIP 103210)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103210
124043Dr. Isabel Schober, Dr. Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy