Strain identifier
BacDive ID: 2739
Type strain: 
Species: Clostridium thermobutyricum
Strain Designation: JW 171K
Strain history: <- J. Wiegel, JW 171K
NCBI tax ID(s): 1121339 (strain), 29372 (species)
version 8.1 (current version)
General
@ref: 1881
BacDive-ID: 2739
DSM-Number: 4928
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic
description: Clostridium thermobutyricum JW 171K is an anaerobe, thermophilic bacterium that was isolated from horse manure compost.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 29372 | species |
| 1121339 | strain |
strain history
- @ref: 1881
- history: <- J. Wiegel, JW 171K
doi: 10.13145/bacdive2739.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium thermobutyricum
- full scientific name: Clostridium thermobutyricum Wiegel et al. 1989
@ref: 1881
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Clostridium
species: Clostridium thermobutyricum
full scientific name: Clostridium thermobutyricum Wiegel et al. 1989
strain designation: JW 171K
type strain: yes
Culture and growth conditions
culture medium
- @ref: 1881
- name: THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144)
- growth: yes
- link: https://mediadive.dsmz.de/medium/144
- composition: Name: THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144) Composition: Trypticase peptone 10.0 g/l D-Glucose 5.0 g/l Yeast extract 3.0 g/l K2HPO4 1.5 g/l Na2S x 9 H2O 1.0 g/l NH4Cl 0.9 g/l KH2PO4 0.75 g/l MgCl2 x 6 H2O 0.4 g/l Nitrilotriacetic acid 0.1152 g/l NaCl 0.009 g/l FeSO4 x 7 H2O 0.003 g/l FeCl2 x 4 H2O 0.0018 g/l CoCl2 x 6 H2O 0.00153 g/l MnCl2 x 4 H2O 0.0009 g/l CaCl2 x 2 H2O 0.0009 g/l ZnCl2 0.0009 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.00027 g/l Na2SeO3 x 5 H2O 0.00027 g/l CuCl2 0.00018 g/l H3BO3 9e-05 g/l Na2MoO4 x 2 H2O 9e-05 g/l Pyridoxine hydrochloride 5e-05 g/l p-Aminobenzoic acid 2.5e-05 g/l (DL)-alpha-Lipoic acid 2.5e-05 g/l Riboflavin 2.5e-05 g/l Calcium D-(+)-pantothenate 2.5e-05 g/l Thiamine HCl 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l Biotin 1e-05 g/l Folic acid 1e-05 g/l Vitamin B12 5e-07 g/l Distilled water
culture temp
- @ref: 1881
- growth: positive
- type: growth
- temperature: 55
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 1881
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 1881
- sample type: horse manure compost
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Biodegradation | #Composting |
| #Host | #Mammals | #Equidae (Horse) |
| #Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
taxonmaps
- @ref: 69479
- File name: preview.99_25475.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_3674;97_15461;98_19150;99_25475&stattab=map
- Last taxonomy: Clostridium
- 16S sequence: LT626259
- Sequence Identity:
- Total samples: 2556
- soil counts: 540
- aquatic counts: 267
- animal counts: 1451
- plant counts: 298
Safety information
risk assessment
- @ref: 1881
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 1881 | Clostridium thermobutyricum partial 16S rRNA gene, type strain DSM4928T, isolate Clone1 | LT626252 | 1503 | ena | 29372 |
| 1881 | Clostridium thermobutyricum partial 16S rRNA gene, type strain DSM4928T, isolate Clone 2 | LT626253 | 1504 | ena | 29372 |
| 1881 | Clostridium thermobutyricum partial 16S rRNA gene, type strain DSM4928T, isolate Clone 3 | LT626254 | 1504 | ena | 29372 |
| 1881 | Clostridium thermobutyricum partial 16S rRNA gene, type strain DSM4928T, isolate Clone 4 | LT626255 | 1504 | ena | 29372 |
| 1881 | Clostridium thermobutyricum partial 16S rRNA gene, type strain DSM4928T, isolate Clone 5 | LT626256 | 1504 | ena | 29372 |
| 1881 | Clostridium thermobutyricum partial 16S rRNA gene, type strain DSM4928T, isolate Clone 6 | LT626257 | 1501 | ena | 29372 |
| 1881 | Clostridium thermobutyricum partial 16S rRNA gene, type strain DSM4928T, isolate Clone 7 | LT626258 | 1503 | ena | 29372 |
| 1881 | Clostridium thermobutyricum partial 16S rRNA gene, type strain DSM4928T, isolate Clone 8 | LT626259 | 1505 | ena | 29372 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Clostridium thermobutyricum DSM 4928 | GCA_002050515 | contig | ncbi | 1121339 |
| 66792 | Clostridium thermobutyricum DSM 4928 strain DSM 4928 | 1121339.3 | wgs | patric | 1121339 |
| 66792 | Clostridium thermobutyricum DSM 4928 | 2916599234 | draft | img | 29372 |
GC content
- @ref: 1881
- GC-content: 37.0
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 68.648 | no |
| flagellated | no | 73.186 | no |
| gram-positive | yes | 84.829 | no |
| anaerobic | yes | 97.177 | yes |
| aerobic | no | 96.412 | yes |
| halophile | no | 82.825 | no |
| spore-forming | yes | 91.456 | no |
| thermophile | no | 95.13 | yes |
| glucose-util | yes | 90.822 | no |
| glucose-ferment | yes | 70.895 | no |
External links
@ref: 1881
culture collection no.: DSM 4928, ATCC 49875
straininfo link
- @ref: 72268
- straininfo: 42730
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Genetics | 28522720 | First Insight into the Genome Sequence of Clostridium thermobutyricum DSM 4928, a Butyrate-Producing Moderate Thermophile. | Poehlein A, Anbalagan A, Nagel A, Daniel R | Genome Announc | 10.1128/genomeA.00367-17 | 2017 | ||
| Phylogeny | 30058994 | Clostridium bovifaecis sp. nov., a novel acetogenic bacterium isolated from cow manure. | Zhu H, Fu B, Lu S, Liu H, Liu H | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002928 | 2018 | Animals, Bacterial Typing Techniques, Base Composition, Cattle/*microbiology, Clostridium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Manure/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1881 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4928) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4928 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 72268 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42730.1 | StrainInfo: A central database for resolving microbial strain identifiers |