Strain identifier
BacDive ID: 2717
Type strain:
Species: Acetoanaerobium sticklandii
Strain Designation: HF
Strain history: ATCC 12662 <-- A. C. Schwartz <-- T. C. Stadtman.
NCBI tax ID(s): 499177 (strain), 1511 (species)
General
@ref: 263
BacDive-ID: 2717
DSM-Number: 519
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive
description: Acetoanaerobium sticklandii HF is an anaerobe, spore-forming, mesophilic bacterium that was isolated from black mud.
NCBI tax id
NCBI tax id | Matching level |
---|---|
499177 | strain |
1511 | species |
strain history
@ref | history |
---|---|
263 | <- A. C. Schwartz, Univ. Bonn, Germany; HF <- T. C. Stadtman, National Institute of Health, Bethesda, Md., USA |
67770 | ATCC 12662 <-- A. C. Schwartz <-- T. C. Stadtman. |
doi: 10.13145/bacdive2717.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Peptostreptococcaceae
- genus: Acetoanaerobium
- species: Acetoanaerobium sticklandii
- full scientific name: Acetoanaerobium sticklandii (Stadtman and McClung 1957) Galperin et al. 2016
synonyms
- @ref: 20215
- synonym: Clostridium sticklandii
@ref: 263
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Peptostreptococcaceae
genus: Acetoanaerobium
species: Acetoanaerobium sticklandii
full scientific name: Acetoanaerobium sticklandii (Stadtman and McClung 1957) Galperin et al. 2016
strain designation: HF
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 99.72
Culture and growth conditions
culture medium
- @ref: 263
- name: ACETOANAEROBIUM MEDIUM (DSMZ Medium 38)
- growth: yes
- link: https://mediadive.dsmz.de/medium/38
- composition: Name: ACETOANAEROBIUM MEDIUM (DSMZ Medium 38) Composition: Tryptone 20.0 g/l Yeast extract 10.0 g/l K2HPO4 1.04 g/l KH2PO4 0.68 g/l Na2S x 9 H2O 0.15 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
263 | positive | growth | 35 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
263 | anaerobe | |
69480 | anaerobe | 99.68 |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 99 |
69480 | no | 96.141 |
compound production
- @ref: 263
- compound: glycine reductase
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
25060 | black mud | east shore of San Francisco Bay | USA | USA | North America |
263 | mud water | east shore of San Francisco Bay | USA | USA | North America |
67770 | Mud water |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Mud (Sludge)
taxonmaps
- @ref: 69479
- File name: preview.99_1599.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15797;96_907;97_1057;98_1263;99_1599&stattab=map
- Last taxonomy: Acetoanaerobium sticklandii
- 16S sequence: LC053841
- Sequence Identity:
- Total samples: 20854
- soil counts: 1323
- aquatic counts: 13161
- animal counts: 6000
- plant counts: 370
Safety information
risk assessment
- @ref: 263
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
67770 | [Clostridium] sticklandii gene for 16S ribosomal RNA, partial sequence, strain: JCM 1433 | LC053841 | 1388 | ena | 1511 |
67770 | C.sticklandii small subunit ribosomal RNA | M26494 | 1476 | ena | 1511 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Acetoanaerobium sticklandii DSM 519 | GCA_000196455 | complete | ncbi | 1511 |
66792 | Clostridium sticklandii DSM 519 | 499177.3 | complete | patric | 499177 |
66792 | Acetoanaerobium sticklandii DSM 519 | 649633033 | complete | img | 1511 |
GC content
@ref | GC-content |
---|---|
25060 | 33.3 |
263 | 31.0 |
67770 | 31 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 99 | no |
gram-positive | yes | 71.928 | no |
anaerobic | yes | 98.862 | yes |
halophile | no | 87.852 | no |
spore-forming | yes | 87.029 | no |
glucose-util | yes | 86.689 | no |
aerobic | no | 97.551 | yes |
thermophile | no | 95.391 | no |
motile | yes | 88.044 | no |
flagellated | yes | 50.639 | no |
glucose-ferment | yes | 51.307 | no |
External links
@ref: 263
culture collection no.: DSM 519, ATCC 12662, CCUG 9281, JCM 1433, NCIMB 10654, BCRC 14485
straininfo link
- @ref: 72245
- straininfo: 35630
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 25948619 | Acetoanaerobium pronyense sp. nov., an anaerobic alkaliphilic bacterium isolated from a carbonate chimney of the Prony Hydrothermal Field (New Caledonia). | Bes M, Merrouch M, Joseph M, Quemeneur M, Payri C, Pelletier B, Ollivier B, Fardeau ML, Erauso G, Postec A | Int J Syst Evol Microbiol | 10.1099/ijs.0.000307 | 2015 | Bacterial Typing Techniques, Base Composition, Carbonates, Clostridiales/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Hydrothermal Vents/*microbiology, Molecular Sequence Data, New Caledonia, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Genetics |
Genetics | 31425748 | Functional prediction, characterization, and categorization of operome from Acetoanaerobium sticklandii DSM 519. | Sangavai C, Prathiviraj R, Chellapandi P | Anaerobe | 10.1016/j.anaerobe.2019.102088 | 2019 | Amino Acid Motifs, Amino Acid Sequence, Bacterial Proteins/chemistry/genetics/metabolism, Clostridiales/*physiology, Computational Biology/methods, Conserved Sequence, Energy Metabolism, Gene Expression Regulation, Bacterial, *Genome, Bacterial, *Genomics/methods, Molecular Sequence Annotation, *Operon, Stress, Physiological, Transcription, Genetic | Metabolism |
Metabolism | 31471743 | A metabolic study to decipher amino acid catabolism-directed biofuel synthesis in Acetoanaerobium sticklandii DSM 519. | Sangavai C, Chellapandi P | Amino Acids | 10.1007/s00726-019-02777-4 | 2019 | Acetic Acid/metabolism, Acetone/metabolism, Amino Acids/chemistry/*metabolism, *Biofuels, Butanols/metabolism, Butyric Acid/metabolism, Chromatography, High Pressure Liquid, Clostridiales/*metabolism, Ethanol/metabolism, Fermentation, Gas Chromatography-Mass Spectrometry, Gelatin/*metabolism, Metabolomics, Solvents/chemistry/metabolism | |
Metabolism | 32812241 | Growth-associated catabolic potential of Acetoanaerobium sticklandii DSM 519 on gelatin and amino acids. | Sangavai C, Chellapandi P | J Basic Microbiol | 10.1002/jobm.202000292 | 2020 | Amino Acids/chemistry/*metabolism, Ammonia/metabolism, Biofuels, Clostridiales/*growth & development/*metabolism, Feedback, Physiological, Fermentation, Gelatin/*metabolism, Hydrolysis, Kinetics, Substrate Specificity | Enzymology |
Genetics | 34780936 | Comparative genomic analysis of hyper-ammonia producing Acetoanaerobium sticklandii DSM 519 with purinolytic Gottschalkia acidurici 9a and pathogenic Peptoclostridium difficile 630. | Sangavai C, Chellapandi P | Genomics | 10.1016/j.ygeno.2021.11.010 | 2021 | *Ammonia/metabolism, *Clostridiales/metabolism, Clostridium, Firmicutes, Genome, Bacterial, Genomics, Phylogeny | Metabolism |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
263 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 519) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-519 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
25060 | M. Y. B. Galperin, V.,Tolstoy, I.,Yutin, N. | 10.1099/ijsem.0.001548 | Phylogenomic analysis of the family Peptostreptococcaceae (Clostridium cluster XI) and proposal for reclassification of Clostridium litorale (Fendrich et al. 1991) and Eubacterium acidaminophilum (Zindel et al. 1989) as Peptoclostridium litorale gen. nov. comb. nov. and Peptoclostridium acidaminophilum comb. nov | IJSEM 66: 5506-5513 2016 | 27902180 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
72245 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID35630.1 | StrainInfo: A central database for resolving microbial strain identifiers |