Strain identifier

BacDive ID: 271

Type strain: Yes

Species: Pseudaeromonas sharmana

Strain Designation: GPTSA-6

Strain history: CIP <- 2006, DSMZ <- MTCC <- P. Saha, India: strain GPTSA-6

NCBI tax ID(s): 328412 (species)

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General

@ref: 6986

BacDive-ID: 271

DSM-Number: 17445

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Pseudaeromonas sharmana GPTSA-6 is a facultative anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from warm spring water.

NCBI tax id

  • NCBI tax id: 328412
  • Matching level: species

strain history

@refhistory
6986<- T. Chakrabarti, MTCC <- P. Saha; GPTSA-6
122582CIP <- 2006, DSMZ <- MTCC <- P. Saha, India: strain GPTSA-6

doi: 10.13145/bacdive271.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Pseudaeromonas
  • species: Pseudaeromonas sharmana
  • full scientific name: Pseudaeromonas sharmana (Saha and Chakrabarti 2006) Padakandla and Chae 2017

  • synonyms

    • @ref: 20215
    • synonym: Aeromonas sharmana

@ref: 6986

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Aeromonadales

family: Aeromonadaceae

genus: Pseudaeromonas

species: Pseudaeromonas sharmana

full scientific name: Pseudaeromonas sharmana (Saha and Chakrabarti 2006) Padakandla and Chae 2017

strain designation: GPTSA-6

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23258negative1-2 µm0.4-0.5 µmrod-shapedyesmonotrichous, polar
25256negative5 µm1-2 µmrod-shapedyespolar
122582negativerod-shapedyes

colony morphology

  • @ref: 23258
  • colony color: whitish
  • colony shape: circular
  • incubation period: 1.5 days
  • medium used: TSBA

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6986TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
6986NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
23258TSBAyes
25256Mueller Hinton agaryes
25256Mueller Hinton brothyes
37534MEDIUM 9 - Brain heart infusion BHIyesDistilled water make up to (1000.000 ml);Brain heart infusion (37.000 g)
122582CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
122582CIP Medium 9yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=9

culture temp

@refgrowthtypetemperaturerange
6986positivegrowth30mesophilic
23258positivegrowth15psychrophilic
23258positivegrowth42thermophilic
23258mesophilic
25256positivegrowth10-45
37534positivegrowth30mesophilic

culture pH

@refabilitytypepH
23258positivegrowth5.7-8.0
25256positivegrowth6.0-8.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23258facultative anaerobe
122582facultative anaerobe

spore formation

  • @ref: 25256
  • type of spore: endospore
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
23258NaClpositivemaximum2 %
25256NaClmaximum4 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2325815963ribitol-builds acid from
2325817108D-arabinose-builds acid from
232586731melezitose-builds acid from
2325828053melibiose-builds acid from
2325816634raffinose-builds acid from
2325816988D-ribose-builds acid from
2325817924D-sorbitol-builds acid from
2325865327D-xylose-builds acid from
2325816813galactitol-builds acid from
2325817754glycerol-builds acid from
2325830849L-arabinose-builds acid from
2325818403L-arabitol-builds acid from
2325862345L-rhamnose-builds acid from
2325817266L-sorbose-builds acid from
2325865328L-xylose-builds acid from
2325817268myo-inositol-builds acid from
2325817151xylitol-builds acid from
2325817234glucose-builds gas from
2325817019(R)-amygdalin-carbon source
2325830089acetate-carbon source
2325815963ribitol-carbon source
2325818305arbutin-carbon source
2325816947citrate-carbon source
2325817108D-arabinose-carbon source
2325816988D-ribose-carbon source
2325817924D-sorbitol-carbon source
2325816813galactitol-carbon source
2325829806fumarate-carbon source
2325817859glutaric acid-carbon source
2325817754glycerol-carbon source
2325818403L-arabitol-carbon source
2325862345L-rhamnose-carbon source
2325817266L-sorbose-carbon source
2325825115malate-carbon source
232586731melezitose-carbon source
2325817272propionate-carbon source
2325830031succinate-carbon source
23258casein-hydrolysis
2325853424tween 20-hydrolysis
2325853423tween 40-hydrolysis
2325853425tween 60-hydrolysis
2325853426tween 80-hydrolysis
2325816199urea-hydrolysis
2325817632nitrate-reduction
2325817019(R)-amygdalin+builds acid from
2325818305arbutin+builds acid from
2325817057cellobiose+builds acid from
2325812936D-galactose+builds acid from
2325817634D-glucose+builds acid from
2325817306maltose+builds acid from
2325816024D-mannose+builds acid from
2325828757fructose+builds acid from
2325815443inulin+builds acid from
2325817814salicin+builds acid from
2325817992sucrose+builds acid from
2325827082trehalose+builds acid from
2325817057cellobiose+carbon source
2325815824D-fructose+carbon source
2325812936D-galactose+carbon source
2325817634D-glucose+carbon source
2325817716lactose+carbon source
2325817306maltose+carbon source
2325816024D-mannose+carbon source
2325830849L-arabinose+carbon source
2325817992sucrose+carbon source
232584853esculin+hydrolysis
23258O-nitrophenyl-beta-D-galactopyranosid+hydrolysis
2325828017starch+hydrolysis
2325865327D-xylose+/-carbon source
2525613705acetoacetate-carbon source
2525618305arbutin-carbon source
2525616947citrate-assimilation
252565291gelatin-hydrolysis
2525630849L-arabinose-carbon source
2525617632nitrate-reduction
2525617309pectin-hydrolysis
2525633942ribose-carbon source
2525653423tween 40-hydrolysis
2525616199urea-hydrolysis
25256739183-O-methyl-D-glucose+carbon source
2525627613amygdalin+carbon source
252564853esculin+hydrolysis
2525628066gentiobiose+carbon source
2525617234glucose+fermentation
2525637684mannose+carbon source
2525617814salicin+carbon source
2525628017starch+hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
6836830849L-arabinose-fermentation
6836827613amygdalin+fermentation
6836828053melibiose-fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
12258217632nitrate-reduction
12258216301nitrite-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
232586472lincomycinyesyes2 µg
232586827methicillinyesyes5 µg
2325873908vibriostatyesyes
2325818208penicillin gyesyes10 Unit
232588309polymyxin byesyes300 Unit
2325828669bacitracinyesyes8 Unit
2325828971ampicillinyesyes10 µg
2325837943colistinyesyes10 µg
2325817833gentamicinyesyes10 µg
2325817076streptomycinyesyes10 µg
2325848923erythromycinyesyes15 µg
2325828077rifampicinyesyes2 µg
2325827902tetracyclineyesyes3 µg
232583542cephalothinyesyes30 µg
2325817698chloramphenicolyesyes30 µg
232586104kanamycinyesyes30 µg
232587507neomycinyesyes30 µg
2325828368novobiocinyesyes30 µg
2325871415nitrofurantoinyesyes300 µg
2325832166sulfisomidineyesyes300 µg

metabolite production

@refChebi-IDmetaboliteproduction
2325835581indoleno
2325816136hydrogen sulfideno
2525615688acetoinno
2525616136hydrogen sulfideno
2525635581indoleno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
12258235581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-testindole test
2525617234glucose+
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
23258beta-galactosidase+3.2.1.23
23258catalase+/-1.11.1.6
23258cytochrome oxidase+1.9.3.1
23258gelatinase-
23258lysine decarboxylase-4.1.1.18
23258ornithine decarboxylase-4.1.1.17
25256arginine dihydrolase-3.5.3.6
25256beta-galactosidase+3.2.1.23
25256catalase-1.11.1.6
25256cytochrome oxidase-1.9.3.1
25256lysine decarboxylase-4.1.1.18
25256ornithine decarboxylase-4.1.1.17
25256tryptophan deaminase-4.1.99.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
122582oxidase+
122582catalase+1.11.1.6
122582urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25256C12:06.3
    25256C14:05.5
    25256C14:0 3OH / C16:1 iso6.4
    25256C16:029.6
    25256C16:1ω7c / C16:1ω6c26.3
    25256C18:01
    25256C18:1ω7c23.9
  • type of FA analysis: whole cell analysis
  • incubation medium: Mueller Hinton agar
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • software version: Sherlock 6.0
  • system: MIS MIDI
  • method/protocol: Sasser 2001

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122582---+-+-----+-+--+---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
6986+----------++---+-+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122582---------++++----+--+++-+++++-+----++-+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6986warm spring waterAssam, JorhatIndiaINDAsia
122582Environment, Water, warmspringAssam, JohratIndiaINDAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Thermal spring

taxonmaps

  • @ref: 69479
  • File name: preview.99_17173.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_11;96_8819;97_10603;98_13002;99_17173&stattab=map
  • Last taxonomy: Pseudaeromonas sharmana subclade
  • 16S sequence: DQ013306
  • Sequence Identity:
  • Total samples: 854
  • soil counts: 23
  • aquatic counts: 489
  • animal counts: 328
  • plant counts: 14

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
69861Risk group (German classification)
1225821Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6986
  • description: Aeromonas sharmana strain GPTSA-6 16S ribosomal RNA gene, partial sequence
  • accession: DQ013306
  • length: 1430
  • database: ena
  • NCBI tax ID: 328412

GC content

@refGC-contentmethod
2325860.7fluorimetric
2525660.7Thermal denaturation, fluorometry
698660.7thermal denaturation, midpoint method (Tm)

External links

@ref: 6986

culture collection no.: DSM 17445, CCUG 54939, CIP 109378, MTCC 7090

straininfo link

  • @ref: 69953
  • straininfo: 297117

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16902029Aeromonas sharmana sp. nov., isolated from a warm spring.Saha P, Chakrabarti TInt J Syst Evol Microbiol10.1099/ijs.0.63972-02006Aeromonas/chemistry/*classification/isolation & purification/physiology, India, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Species Specificity, *Water MicrobiologyGenetics
Phylogeny17407062The recently proposed species Aeromonas sharmana sp. nov., isolate GPTSA-6T, is not a member of the genus Aeromonas.Martinez-Murcia AJ, Figueras MJ, Saavedra MJ, Stackebrandt EInt Microbiolim23060492007Aeromonadaceae/*classification/genetics, DNA, Bacterial/chemistry/genetics, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/chemistry/genetics, Sequence Alignment, Sequence Analysis, DNATranscriptome
Phylogeny27974085Reclassification of Aeromonas sharmana to a new genus as Pseudaeromonas sharmana gen. nov., comb. nov., and description of Pseudaeromonas pectinilytica sp. nov. isolated from a freshwater stream.Padakandla SR, Chae JCInt J Syst Evol Microbiol10.1099/ijsem.0.0017342017Aeromonadaceae/*classification/genetics/isolation & purification, Aeromonas/*classification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rivers/*microbiology, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6986Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17445)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17445
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23258P. Saha, T. Chakrabarti10.1099/ijs.0.63972-0Aeromonas sharmana sp. nov., isolated from a warm springIJSEM 56: 1905-1909 200616902029
25256S. R. C. Padakandla, J. C.10.1099/ijsem.0.001734Reclassification of Aeromonas sharmana to a new genus as Pseudaeromonas sharmana gen. nov., comb. nov., and description of Pseudaeromonas pectinilytica sp. nov. isolated from a freshwater streamIJSEM 67: 1018-1023 201727974085
37534Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7112
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69953Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297117.1StrainInfo: A central database for resolving microbial strain identifiers
122582Curators of the CIPCollection of Institut Pasteur (CIP 109378)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109378