General

@ref: 1125

BacDive-ID: 2673

DSM-Number: 2575

keywords: Bacteria, anaerobe, thermophilic

description: Clostridium sp. RB-9 is an anaerobe, thermophilic bacterium that was isolated from mud hot spring.

NCBI tax id

• NCBI tax id: 1506
• Matching level: species

strain history

• @ref: 1125
• history: <- J. Wiegel, RB-9 ("Clostridium thermoamylolyticum")

doi: 10.13145/bacdive2673.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacillota
• domain: Bacteria
• phylum: Bacillota
• class: Clostridia
• order: Eubacteriales
• family: Clostridiaceae
• genus: Clostridium
• species: Clostridium sp.
• full scientific name: Clostridium Prazmowski 1880 (Approved Lists 1980)

• synonyms

  @ref	synonym
  20215	Tyzzerella
  20215	Pseudoclostridium
  20215	Lachnoclostridium
  20215	Anaerobacter
  20215	Inflabilis

@ref: 1125

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium sp.

full scientific name: Clostridium sp.

strain designation: RB-9

type strain: no

Culture and growth conditions

culture medium

• @ref: 1125
• name: CLOSTRIDIUM (RB-9) MEDIUM (DSMZ Medium 558a)
• growth: yes
• link: https://mediadive.dsmz.de/medium/558a
• composition: Name: CLOSTRIDIUM (RB-9) MEDIUM (DSMZ Medium 558a) Composition: D-Glucose 9.8912 g/l Na2HPO4 x 12 H2O 4.1543 g/l Yeast extract 1.97824 g/l KH2PO4 1.48368 g/l Na2SO4 0.98912 g/l NH4Cl 0.49456 g/l Na2S x 9 H2O 0.49456 g/l L-Cysteine HCl x H2O 0.49456 g/l MgCl2 x 6 H2O 0.356083 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l ZnSO4 x 7 H2O 0.00178042 g/l CoSO4 x 7 H2O 0.00178042 g/l FeSO4 x 7 H2O 0.00098912 g/l CaCl2 x 2 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l H3BO3 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l CuSO4 x 5 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l Thiamine HCl 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Biotin 1.97824e-05 g/l Folic acid 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

• @ref: 1125
• growth: positive
• type: growth
• temperature: 55
• range: thermophilic

Physiology and metabolism

oxygen tolerance

• @ref: 1125
• oxygen tolerance: anaerobe

compound production

• @ref: 1125
• compound: glucoamylase

Isolation, sampling and environmental information

isolation

• @ref: 1125
• sample type: mud hot spring
• geographic location: Hveragerdi
• country: Iceland
• origin.country: ISL
• continent: Europe

isolation source categories

External links

@ref: 1125

culture collection no.: DSM 2575, ATCC 39252

straininfo link

• @ref: 72202
• straininfo: 45084

Reference