Strain identifier

BacDive ID: 2666

Type strain: Yes

Species: Paeniclostridium sordellii

Strain Designation: 211

Strain history: CIP <- 1997, J.P. Carlier, Inst. Pasteur, Paris, France: strain

NCBI tax ID(s): 1292036 (strain), 1505 (species)

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General

@ref: 976

BacDive-ID: 2666

DSM-Number: 2141

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Paeniclostridium sordellii 211 is an anaerobe, spore-forming, mesophilic human pathogen that was isolated from Environment, Spore appendages.

NCBI tax id

NCBI tax idMatching level
1292036strain
1505species

strain history

@refhistory
976<- ATCC <- G.B. Reed, 211
381411997, J.P. Carlier, Inst. Pasteur, Paris, France: strain
67770ATCC 9714 <-- G. B. Reed 211.
122681CIP <- 1997, J.P. Carlier, Inst. Pasteur, Paris, France: strain

doi: 10.13145/bacdive2666.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Paeniclostridium
  • species: Paeniclostridium sordellii
  • full scientific name: Paeniclostridium sordellii (Hall and Scott 1927) Sasi Jyothsna et al. 2016

  • synonyms

    @refsynonym
    20215Clostridium sordellii
    20215Bacillus sordelli

@ref: 976

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Paeniclostridium

species: Paeniclostridium sordellii

full scientific name: Paeniclostridium sordellii (Hall and Scott 1927) Sasi Jyothsna et al. 2016

strain designation: 211

type strain: yes

Morphology

cell morphology

  • @ref: 122681
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refincubation periodhemolysis ability
451831 day
1226811

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
976CHOPPED MEAT MEDIUM (DSMZ Medium 78)yeshttps://mediadive.dsmz.de/medium/78Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
38141MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
122681CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6
122681CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
976positivegrowth37mesophilic
38141positivegrowth37mesophilic
45183positivegrowth37mesophilic
67770positivegrowth37mesophilic
122681positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
976anaerobe
45183anaerobe
45183obligate anaerobe
122681anaerobe

spore formation

  • @ref: 122681
  • spore formation: yes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12268117108D-arabinose-degradation
12268115824D-fructose+degradation
12268117634D-glucose+degradation
12268165327D-xylose-degradation
12268117057cellobiose-degradation
12268117716lactose+degradation
12268117306maltose+degradation
12268117814salicin-degradation
12268117992sucrose+degradation
1226814853esculin+hydrolysis
12268117632nitrate-reduction
12268116301nitrite-reduction
12268117632nitrate+respiration

metabolite production

  • @ref: 122681
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122681oxidase-
122681beta-galactosidase+3.2.1.23
122681gelatinase+/-
122681amylase+
122681DNase+
122681caseinase+3.4.21.50
122681catalase-1.11.1.6
122681tween esterase-
122681lecithinase+
122681lipase-
122681protease-
122681urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122681-+++----+-++++-+----

Isolation, sampling and environmental information

isolation

  • @ref: 122681
  • sample type: Environment, Spore appendages

taxonmaps

  • @ref: 69479
  • File name: preview.99_1682.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_2;96_337;97_376;98_1330;99_1682&stattab=map
  • Last taxonomy: Paeniclostridium sordellii
  • 16S sequence: AB550230
  • Sequence Identity:
  • Total samples: 46616
  • soil counts: 4480
  • aquatic counts: 4522
  • animal counts: 35570
  • plant counts: 2044

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
976yesyes2Risk group (German classification)
1226812Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Clostridium sordellii 16S ribosomal RNAM591051454ena1505
976[Clostridium] sordellii gene for 16S rRNA, partial sequence, strain: JCM 3814AB5502301463ena1505

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Paeniclostridium sordellii ATCC 9714GCA_000953675chromosomencbi1505
66792Paeniclostridium sordellii ATCC 9714GCA_000444075contigncbi1292036
66792[Clostridium] sordellii strain ATCC97141505.8completepatric1505
66792[Clostridium] sordellii strain JGS63821505.7completepatric1505
66792Clostridium sordellii ATCC 97141292036.3wgspatric1292036
66792Paeniclostridium sordellii strain JGS63641505.66wgspatric1505
66792Paeniclostridium sordellii strain JGS63821505.56plasmidpatric1505
66792Paeniclostridium sordellii strain UMC21505.67wgspatric1505
66792Paeniclostridium sordellii ATCC 97142651869763completeimg1292036
66792Paeniclostridium sordellii ATCC 97142563367177draftimg1292036

External links

@ref: 976

culture collection no.: DSM 2141, ATCC 9714, NCIB 10717, CCUG 9284, LMG 15708, CIP 103658, BCRC 10649, JCM 3814, NCIMB 10717

straininfo link

  • @ref: 72195
  • straininfo: 92518

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phenotype3911662Correlative properties for a differentiation of two Clostridium sordellii phenotypes and their distinction from Clostridium bifermentans.Roggentin P, Gutschker-Gdaniec G, Schauer R, Hobrecht RZentralbl Bakteriol Mikrobiol Hyg A10.1016/s0176-6724(85)80020-11985Animals, Bacteriological Techniques, Clostridium/classification/*genetics/pathogenicity, Culture Media, Humans, Mice, Phenotype, VirulenceCultivation
Enzymology8050999Characterization and regulation of the NADP-linked 7 alpha-hydroxysteroid dehydrogenase gene from Clostridium sordellii.Coleman JP, Hudson LL, Adams MJJ Bacteriol10.1128/jb.176.16.4865-4874.19941994Amino Acid Sequence, Bacterial Proteins/chemistry/genetics, Base Sequence, Cloning, Molecular, Clostridium/*enzymology/*genetics, DNA, Bacterial/genetics, Gene Expression Regulation, Bacterial, *Genes, Bacterial, Hydroxysteroid Dehydrogenases/*genetics, Molecular Sequence Data, NADP, Oligodeoxyribonucleotides/chemistry, RNA, Messenger/genetics, Restriction Mapping, Sequence Alignment, Sequence Homology, Amino AcidGenetics
Phylogeny812355616S ribosomal DNA sequences of anaerobic cocci and proposal of Ruminococcus hansenii comb. nov. and Ruminococcus productus comb. nov.Ezaki T, Li N, Hashimoto Y, Miura H, Yamamoto HInt J Syst Bacteriol10.1099/00207713-44-1-1301994Anaerobiosis, Base Sequence, DNA, Bacterial/*chemistry, DNA, Ribosomal/*chemistry, Molecular Sequence Data, Peptococcaceae/*classification/*genetics, RNA, Ribosomal, 16S/*geneticsGenetics
Pathogenicity19527792Lethal toxin is a critical determinant of rapid mortality in rodent models of Clostridium sordellii endometritis.Hao Y, Senn T, Opp JS, Young VB, Thiele T, Srinivas G, Huang SK, Aronoff DMAnaerobe10.1016/j.anaerobe.2009.06.0022009Animals, Antitoxins/administration & dosage, Bacterial Toxins/antagonists & inhibitors/genetics/*toxicity, Clostridium Infections/*microbiology/*mortality/prevention & control, Clostridium sordellii/genetics/isolation & purification/metabolism/*pathogenicity, Endometritis/*microbiology/*mortality/prevention & control, Female, Gene Expression Profiling, Humans, Immunoglobulin G/administration & dosage, Mice, Mice, Inbred C57BL, Rats, Rats, Wistar, Virulence Factors/genetics/toxicityEnzymology
Stress20152919Heat resistance of Clostridium sordellii spores.Kozma-Sipos Z, Szigeti J, Asvanyi B, Varga LAnaerobe10.1016/j.anaerobe.2010.01.0022010Clostridium sordellii/*physiology, Food Handling/methods, *Hot Temperature, *Microbial Viability, Spores, Bacterial/physiology, Time Factors
Proteome20338254Comparative analysis of the extracellular proteomes of two Clostridium sordellii strains exhibiting contrasting virulence.Kachman MT, Hurley MC, Thiele T, Srinivas G, Aronoff DMAnaerobe10.1016/j.anaerobe.2010.03.0042010Bacterial Proteins/*analysis/*metabolism, Bacterial Toxins/genetics, Clostridium sordellii/*chemistry/pathogenicity, Electrophoresis, Gel, Two-Dimensional, Mass Spectrometry, Proteome/*analysis, Virulence Factors/*analysisEnzymology
Pathogenicity21199912TcsL is an essential virulence factor in Clostridium sordellii ATCC 9714.Carter GP, Awad MM, Hao Y, Thelen T, Bergin IL, Howarth PM, Seemann T, Rood JI, Aronoff DM, Lyras DInfect Immun10.1128/IAI.00968-102011Animals, Bacterial Toxins/*genetics, Blotting, Southern, Blotting, Western, Chlorocebus aethiops, Clostridium sordellii/*genetics/pathogenicity, Genes, Bacterial/genetics, Mice, Mice, Inbred BALB C, Polymerase Chain Reaction, Vero Cells, Virulence, Virulence Factors/*genetics
Metabolism23873908Identification and characterization of Clostridium sordellii toxin gene regulator.Sirigi Reddy AR, Girinathan BP, Zapotocny R, Govind RJ Bacteriol10.1128/JB.00711-132013Animals, *Bacterial Proteins/chemistry/genetics/metabolism, Bacterial Toxins/genetics/*metabolism, Clostridium Infections/microbiology, Clostridium sordellii/genetics/metabolism/pathogenicity, Female, *Gene Expression Regulation, Bacterial, Genes, Regulator, Genome, Bacterial, High-Throughput Nucleotide Sequencing, Humans, Molecular Sequence Data, Sequence Analysis, DNA, *Sigma Factor/chemistry/genetics/metabolism, Virulence Factors/chemistry/genetics/metabolismGenetics
Phylogeny25981746Clostridium sordellii genome analysis reveals plasmid localized toxin genes encoded within pathogenicity loci.Couchman EC, Browne HP, Dunn M, Lawley TD, Songer JG, Hall V, Petrovska L, Vidor C, Awad M, Lyras D, Fairweather NFBMC Genomics10.1186/s12864-015-1613-22015Bacterial Toxins/*genetics, Chromosome Mapping, Clostridium sordellii/classification/*genetics/*pathogenicity, Gene Transfer, Horizontal, Genetic Loci/genetics, Genome, Bacterial/*genetics, Neuraminidase/genetics, Phylogeny, Plasmids/genetics/*metabolism, Sequence Analysis, DNA, Type C Phospholipases/genetics, Virulence Factors/*geneticsGenetics
Phylogeny26738915Paraclostridium benzoelyticum gen. nov., sp. nov., isolated from marine sediment and reclassification of Clostridium bifermentans as Paraclostridium bifermentans comb. nov. Proposal of a new genus Paeniclostridium gen. nov. to accommodate Clostridium sordellii and Clostridium ghonii.Sasi Jyothsna TS, Tushar L, Sasikala C, Ramana CVInt J Syst Evol Microbiol10.1099/ijsem.0.0008742016
Metabolism26805011A novel murine model of Clostridium sordellii myonecrosis: Insights into the pathogenesis of disease.Aldape MJ, Bayer CR, Bryant AE, Stevens DLAnaerobe10.1016/j.anaerobe.2016.01.0042016Animals, Clostridium Infections/metabolism/*microbiology/mortality/*pathology, *Clostridium sordellii/pathogenicity, Cytokines, Disease Models, Animal, Leukocyte Count, Mice, Mortality, Muscle, Skeletal/*microbiology/*pathology, Necrosis
Pathogenicity27322322The Sialidase NanS Enhances Non-TcsL Mediated Cytotoxicity of Clostridium sordellii.Awad MM, Singleton J, Lyras DToxins (Basel)10.3390/toxins80601892016Bacterial Proteins/genetics/*toxicity, Bacterial Toxins/*genetics/toxicity, Caco-2 Cells, Cell Line, Cell Survival/drug effects, Clostridium sordellii/*enzymology/genetics, Humans, Mutation, Neuraminidase/genetics/*toxicityEnzymology
28515901Experimental identification and computational characterization of a novel extracellular metalloproteinase produced by Clostridium sordellii.Aldape MJ, Tao A, Heeney DD, McIndoo ER, French JM, Xu DRSC Adv10.1039/c6ra27654g2017
29339424Clostridium sordellii Pathogenicity Locus Plasmid pCS1-1 Encodes a Novel Clostridial Conjugation Locus.Vidor CJ, Watts TD, Adams V, Bulach D, Couchman E, Rood JI, Fairweather NF, Awad M, Lyras DmBio10.1128/mBio.01761-172018Clostridium sordellii/*genetics, Computational Biology, *Conjugation, Genetic, *Gene Transfer, Horizontal, Genes, Bacterial, *Genetic Loci, Multigene Family, *Plasmids

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
976Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2141)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2141
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38141Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15480
45183Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 9284)https://www.ccug.se/strain?id=9284
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72195Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92518.1StrainInfo: A central database for resolving microbial strain identifiers
122681Curators of the CIPCollection of Institut Pasteur (CIP 103658)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103658