Strain identifier
BacDive ID: 2659
Type strain: 
Species: Clostridium saccharoperbutylacetonicum
Strain Designation: N1-4(HMT)
Strain history: <- ATCC <- S. Ogata; N1-4(HMT) <- M. Hongo and K. Nagata, Kyushu University, Fukuoka, Japan {1959}
NCBI tax ID(s): 36745 (species)
version 8.1 (current version)
General
@ref: 5607
BacDive-ID: 2659
DSM-Number: 14923
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic
description: Clostridium saccharoperbutylacetonicum N1-4 is an anaerobe, mesophilic bacterium that was isolated from soil.
NCBI tax id
- NCBI tax id: 36745
- Matching level: species
strain history
- @ref: 5607
- history: <- ATCC <- S. Ogata; N1-4(HMT) <- M. Hongo and K. Nagata, Kyushu University, Fukuoka, Japan {1959}
doi: 10.13145/bacdive2659.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium saccharoperbutylacetonicum
- full scientific name: Clostridium saccharoperbutylacetonicum Keis et al. 2001
@ref: 5607
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Clostridium
species: Clostridium saccharoperbutylacetonicum
full scientific name: Clostridium saccharoperbutylacetonicum Keis et al. 2001
strain designation: N1-4(HMT)
type strain: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 5607 | CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) | yes | https://mediadive.dsmz.de/medium/110 | Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water |
| 5607 | PY + X MEDIUM (DSMZ Medium 104b) | yes | https://mediadive.dsmz.de/medium/104b | Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water |
culture temp
- @ref: 5607
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 5607
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 5607
- sample type: soil
- geographic location: Fukuoka
- country: Japan
- origin.country: JPN
- continent: Asia
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
- @ref: 5607
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5607
- description: Clostridium saccharoperbutylacetonicum N1-4 16S ribosomal RNA (rrn) gene, partial sequence
- accession: U16122
- length: 1417
- database: ena
- NCBI tax ID: 931276
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Clostridium saccharoperbutylacetonicum N1-4(HMT) | GCA_000340885 | complete | ncbi | 931276 |
| 66792 | Clostridium saccharoperbutylacetonicum N1-4(HMT) ATCC 27021 | GCA_000334435 | contig | ncbi | 931276 |
| 66792 | Clostridium saccharoperbutylacetonicum N1-4(HMT) strain ATCC 27021 | 931276.4 | wgs | patric | 931276 |
| 66792 | Clostridium saccharoperbutylacetonicum N1-4(HMT) strain N1-4(HMT) | 931276.5 | complete | patric | 931276 |
| 66792 | Clostridium saccharoperbutylacetonicum N1-4(HMT) strain N1-4(HMT) strain N1-4(HMT) strain N1-4(HMT) | 931276.25 | plasmid | patric | 931276 |
| 66792 | Clostridium saccharoperbutylacetonicum N1-4(HMT) | 2608642274 | complete | img | 931276 |
| 66792 | Clostridium saccharoperbutylacetonicum N1-4(HMT) | 2551306649 | draft | img | 931276 |
GC content
- @ref: 5607
- GC-content: 31
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | yes | 80.715 | no |
| flagellated | yes | 64.484 | no |
| gram-positive | yes | 61.995 | no |
| anaerobic | yes | 95.214 | no |
| aerobic | no | 96.355 | no |
| halophile | no | 96.988 | no |
| spore-forming | yes | 93.989 | no |
| glucose-util | yes | 89.813 | no |
| thermophile | no | 99.58 | no |
| glucose-ferment | yes | 70.272 | no |
External links
@ref: 5607
culture collection no.: DSM 14923, ATCC 27021, NCIMB 12606
straininfo link
- @ref: 72188
- straininfo: 44738
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 11760952 | Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov. and Clostridium saccharobutylicum sp. nov. | Keis S, Shaheen R, Jones DT | Int J Syst Evol Microbiol | 10.1099/00207713-51-6-2095 | 2001 | Acetone/*metabolism, Bacterial Typing Techniques, Butanols/*metabolism, Carbohydrate Metabolism, Clostridium/*classification/genetics/metabolism, DNA Fingerprinting/methods, DNA, Ribosomal/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, Solvents/*metabolism | Metabolism |
| Genetics | 25323722 | Complete Genome Sequence of the Solvent Producer Clostridium saccharoperbutylacetonicum Strain DSM 14923. | Poehlein A, Krabben P, Durre P, Daniel R | Genome Announc | 10.1128/genomeA.01056-14 | 2014 | ||
| Metabolism | 27836845 | Development of a High-Efficiency Transformation Method and Implementation of Rational Metabolic Engineering for the Industrial Butanol Hyperproducer Clostridium saccharoperbutylacetonicum Strain N1-4. | Herman NA, Li J, Bedi R, Turchi B, Liu X, Miller MJ, Zhang W | Appl Environ Microbiol | 10.1128/AEM.02942-16 | 2016 | Biofuels/*analysis, Butanols/*metabolism, Clostridium/*metabolism, Fermentation, Metabolic Engineering/*methods | Biotechnology |
| Metabolism | 32614389 | Genome sequence analysis of the temperate bacteriophage TBP2 of the solvent producer Clostridium saccharoperbutylacetonicum N1-4 (HMT, ATCC 27021). | Schuler MA, Stegmann BA, Poehlein A, Daniel R, Durre P | FEMS Microbiol Lett | 10.1093/femsle/fnaa103 | 2020 | Acetone/metabolism, Bacteriophages/classification/*genetics/*isolation & purification, Butanols/metabolism, Clostridium/metabolism/*virology, Ethanol/metabolism, Fermentation, *Genome, Viral, Industrial Microbiology, Open Reading Frames, Viral Proteins/genetics | Genetics |
| 33307480 | Three-stage repeated-batch immobilized cell fermentation to produce butanol from non-detoxified sugarcane bagasse hemicellulose hydrolysates. | Chacon SJ, Matias G, Ezeji TC, Maciel Filho R, Mariano AP | Bioresour Technol | 10.1016/j.biortech.2020.124504 | 2020 | Butanols, Cells, Immobilized, Cellulose, Clostridium, Fermentation, Hydrolysis, Polysaccharides, *Saccharum |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5607 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14923) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14923 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 72188 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID44738.1 | StrainInfo: A central database for resolving microbial strain identifiers |