Strain identifier

BacDive ID: 265

Type strain: Yes

Species: Aeromonas eucrenophila

Strain Designation: 546

Strain history: CIP <- 1976, M.Y. Popoff, Inst. Pasteur, Paris, France: strain 546 <- NCMB <- J.M. Shevan, Aeromonas punctata <- W. Scharperclaus: strain B, Pseudomonas punctata

NCBI tax ID(s): 649 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 7047

BacDive-ID: 265

DSM-Number: 17534

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, motile, human pathogen

description: Aeromonas eucrenophila 546 is a facultative anaerobe, mesophilic, motile human pathogen that was isolated from fresh water fish.

NCBI tax id

  • NCBI tax id: 649
  • Matching level: species

strain history

@refhistory
7047<- NCIMB <- W. Schaperclaus
67770NCIMB 74 <-- J. M. Shewan <-- W. Schäperclaus strain B.
119137CIP <- 1976, M.Y. Popoff, Inst. Pasteur, Paris, France: strain 546 <- NCMB <- J.M. Shevan, Aeromonas punctata <- W. Scharperclaus: strain B, Pseudomonas punctata

doi: 10.13145/bacdive265.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Aeromonas
  • species: Aeromonas eucrenophila
  • full scientific name: Aeromonas eucrenophila Schubert and Hegazi 1988

@ref: 7047

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Aeromonadales

family: Aeromonadaceae

genus: Aeromonas

species: Aeromonas eucrenophila

full scientific name: Aeromonas eucrenophila Schubert and Hegazi 1988 emend. Huys et al. 1997

strain designation: 546

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.847
6948099.986negative
119137yesnegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7047TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
7047NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
37744MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
119137CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
7047positivegrowth30mesophilic
37744positivegrowth30mesophilic
67770positivegrowth30mesophilic
119137positivegrowth5-41

Physiology and metabolism

oxygen tolerance

  • @ref: 119137
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.906

halophily

@refsaltgrowthtested relationconcentration
119137NaClpositivegrowth0-6 %
119137NaClnogrowth8 %
119137NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68368L-arabinose+fermentation30849
68368amygdalin+fermentation27613
68368melibiose-fermentation28053
68368sucrose-fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol+fermentation16899
68368D-glucose+fermentation17634
68368gelatin+hydrolysis5291
68368tryptophan+energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine+hydrolysis29016
119137mannitol+fermentation29864
119137citrate-carbon source16947
119137esculin+hydrolysis4853
119137glucose+fermentation17234
119137lactose-fermentation17716
119137nitrate+reduction17632
119137nitrite+reduction16301
119137malonate-assimilation15792
119137sodium thiosulfate-builds gas from132112
119137glucose+degradation17234

antibiotic resistance

  • @ref: 119137
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno
11913735581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole+
11913715688acetoin-
11913717234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase+1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
119137oxidase+
119137beta-galactosidase+3.2.1.23
119137alcohol dehydrogenase+1.1.1.1
119137gelatinase+
119137amylase+
119137DNase+
119137caseinase+3.4.21.50
119137catalase+1.11.1.6
119137tween esterase+
119137lecithinase-
119137lipase-
119137lysine decarboxylase-4.1.1.18
119137ornithine decarboxylase-4.1.1.17
119137phenylalanine ammonia-lyase+4.3.1.24
119137protease+
119137tryptophan deaminase-
119137urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119137-+++++----++-+-+++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
7047++------+-+++-----+++

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119137+--++----++++-+/---+---++++++++--+---++-+-------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119137+++++----+++-+-++++++--+--+-+--+-+------------+--+---------++-----------+---+-+++-+---+--++++++--+-

Isolation, sampling and environmental information

isolation

@refsample type
7047fresh water fish
67770Freshwater fish
119137Fresh water fish

isolation source categories

  • Cat1: #Host
  • Cat2: #Fishes

taxonmaps

  • @ref: 69479
  • File name: preview.99_485.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_11;96_15;97_15;98_18;99_485&stattab=map
  • Last taxonomy: Aeromonas
  • 16S sequence: X60411
  • Sequence Identity:
  • Total samples: 313
  • soil counts: 16
  • aquatic counts: 156
  • animal counts: 131
  • plant counts: 10

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
7047yes, in single casesyes, in single cases1Risk group (German classification)
1191371Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218A.eucrenophila (ATCC 23309T) gene for 16S ribosomal RNAX746751460ena649
7047Aeromonas eucrenophila 16S rRNA gene, strain NCIMB 74X604111503ena649

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aeromonas eucrenophila CECT 4224649.3wgspatric649
66792Aeromonas eucrenophila CECT 42242645727995draftimg649
67770Aeromonas eucrenophila CECT 4224GCA_000819865scaffoldncbi649

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno99.05no
anaerobicno97.913no
halophileno91.317no
spore-formingno96.395no
glucose-utilyes91.699no
thermophileno99.591yes
flagellatedyes89.834no
aerobicyes90.063no
motileyes90.196no
glucose-fermentyes89.244yes

External links

@ref: 7047

culture collection no.: DSM 17534, ATCC 23309, CCEB 704, CCTM 885, CDC RH63, CIP 76.17, LMG 3774, NCIMB 74, JCM 8238, BCRC 13017, BCRC 14134, CCM 4354, CCUG 25942, CCUG 30340, CECT 4224, CNCTC 5356

straininfo link

  • @ref: 69947
  • straininfo: 9565

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny9336923Inclusion of Aeromonas DNA hybridization group 11 in Aeromonas encheleia and extended descriptions of the species Aeromonas eucrenophila and A. encheleia.Huys G, Kampfer P, Altwegg M, Coopman R, Janssen P, Gillis M, Kersters KInt J Syst Bacteriol10.1099/00207713-47-4-11571997Aeromonas/*classification/drug effects/genetics/physiology, Base Composition, Culture Media/metabolism, DNA, Bacterial/*analysis, Microbial Sensitivity Tests, Nucleic Acid Hybridization, Phenotype, *PhylogenyPhenotype
Phylogeny31935185Conservation of the name Aeromonas eucrenophila over the name Aeromonas punctata for the organism based on type stain NCMB 74(T) and universally defined as 'Aeromonas DNA hybridization group 6'.Farmer JJ, Holmes BInt J Syst Evol Microbiol10.1099/ijsem.0.0039612020Aeromonas/*classification, DNA, Bacterial/genetics, Nucleic Acid Hybridization, *Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7047Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17534)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17534
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37744Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10786
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69947Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID9565.1StrainInfo: A central database for resolving microbial strain identifiers
119137Curators of the CIPCollection of Institut Pasteur (CIP 76.17)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2076.17