Strain identifier
BacDive ID: 265
Type strain:
Species: Aeromonas eucrenophila
Strain Designation: 546
Strain history: CIP <- 1976, M.Y. Popoff, Inst. Pasteur, Paris, France: strain 546 <- NCMB <- J.M. Shevan, Aeromonas punctata <- W. Scharperclaus: strain B, Pseudomonas punctata
NCBI tax ID(s): 649 (species)
General
@ref: 7047
BacDive-ID: 265
DSM-Number: 17534
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, motile, human pathogen
description: Aeromonas eucrenophila 546 is a facultative anaerobe, mesophilic, motile human pathogen that was isolated from fresh water fish.
NCBI tax id
- NCBI tax id: 649
- Matching level: species
strain history
@ref | history |
---|---|
7047 | <- NCIMB <- W. Schaperclaus |
67770 | NCIMB 74 <-- J. M. Shewan <-- W. Schäperclaus strain B. |
119137 | CIP <- 1976, M.Y. Popoff, Inst. Pasteur, Paris, France: strain 546 <- NCMB <- J.M. Shevan, Aeromonas punctata <- W. Scharperclaus: strain B, Pseudomonas punctata |
doi: 10.13145/bacdive265.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Aeromonadales
- family: Aeromonadaceae
- genus: Aeromonas
- species: Aeromonas eucrenophila
- full scientific name: Aeromonas eucrenophila Schubert and Hegazi 1988
@ref: 7047
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Aeromonadales
family: Aeromonadaceae
genus: Aeromonas
species: Aeromonas eucrenophila
full scientific name: Aeromonas eucrenophila Schubert and Hegazi 1988 emend. Huys et al. 1997
strain designation: 546
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | yes | 98.847 | ||
69480 | 99.986 | negative | ||
119137 | yes | negative | rod-shaped |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
7047 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
7047 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
37744 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
119137 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
7047 | positive | growth | 30 | mesophilic |
37744 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
119137 | positive | growth | 5-41 |
Physiology and metabolism
oxygen tolerance
- @ref: 119137
- oxygen tolerance: facultative anaerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.906 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
119137 | NaCl | positive | growth | 0-6 % |
119137 | NaCl | no | growth | 8 % |
119137 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | + | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | + | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | + | builds acid from | 28087 |
68371 | starch | + | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | - | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | + | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | + | builds acid from | 17057 |
68371 | salicin | + | builds acid from | 17814 |
68371 | esculin | + | builds acid from | 4853 |
68371 | arbutin | + | builds acid from | 18305 |
68371 | amygdalin | + | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | + | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | + | builds acid from | 16988 |
68371 | L-arabinose | + | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | + | builds acid from | 17754 |
68368 | L-arabinose | + | fermentation | 30849 |
68368 | amygdalin | + | fermentation | 27613 |
68368 | melibiose | - | fermentation | 28053 |
68368 | sucrose | - | fermentation | 17992 |
68368 | L-rhamnose | - | fermentation | 62345 |
68368 | sorbitol | - | fermentation | 30911 |
68368 | myo-inositol | - | fermentation | 17268 |
68368 | D-mannitol | + | fermentation | 16899 |
68368 | D-glucose | + | fermentation | 17634 |
68368 | gelatin | + | hydrolysis | 5291 |
68368 | tryptophan | + | energy source | 27897 |
68368 | urea | - | hydrolysis | 16199 |
68368 | citrate | - | assimilation | 16947 |
68368 | ornithine | - | degradation | 18257 |
68368 | lysine | - | degradation | 25094 |
68368 | arginine | + | hydrolysis | 29016 |
119137 | mannitol | + | fermentation | 29864 |
119137 | citrate | - | carbon source | 16947 |
119137 | esculin | + | hydrolysis | 4853 |
119137 | glucose | + | fermentation | 17234 |
119137 | lactose | - | fermentation | 17716 |
119137 | nitrate | + | reduction | 17632 |
119137 | nitrite | + | reduction | 16301 |
119137 | malonate | - | assimilation | 15792 |
119137 | sodium thiosulfate | - | builds gas from | 132112 |
119137 | glucose | + | degradation | 17234 |
antibiotic resistance
- @ref: 119137
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68368 | 15688 | acetoin | no |
68368 | 35581 | indole | yes |
68368 | 16136 | hydrogen sulfide | no |
119137 | 35581 | indole | yes |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test | methylred-test |
---|---|---|---|---|---|
68368 | 15688 | acetoin | - | ||
68368 | 35581 | indole | + | ||
119137 | 15688 | acetoin | - | ||
119137 | 17234 | glucose | + |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68368 | cytochrome oxidase | + | 1.9.3.1 |
68368 | gelatinase | + | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | + | 3.5.3.6 |
68368 | beta-galactosidase | + | 3.2.1.23 |
119137 | oxidase | + | |
119137 | beta-galactosidase | + | 3.2.1.23 |
119137 | alcohol dehydrogenase | + | 1.1.1.1 |
119137 | gelatinase | + | |
119137 | amylase | + | |
119137 | DNase | + | |
119137 | caseinase | + | 3.4.21.50 |
119137 | catalase | + | 1.11.1.6 |
119137 | tween esterase | + | |
119137 | lecithinase | - | |
119137 | lipase | - | |
119137 | lysine decarboxylase | - | 4.1.1.18 |
119137 | ornithine decarboxylase | - | 4.1.1.17 |
119137 | phenylalanine ammonia-lyase | + | 4.3.1.24 |
119137 | protease | + | |
119137 | tryptophan deaminase | - | |
119137 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119137 | - | + | + | + | + | + | - | - | - | - | + | + | - | + | - | + | + | + | - | - |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
7047 | + | + | - | - | - | - | - | - | + | - | + | + | + | - | - | - | - | - | + | + | + |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119137 | + | - | - | + | + | - | - | - | - | + | + | + | + | - | +/- | - | - | + | - | - | - | + | + | + | + | + | + | + | + | - | - | + | - | - | - | + | + | - | + | - | - | - | - | - | - | - | + | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119137 | + | + | + | + | + | - | - | - | - | + | + | + | - | + | - | + | + | + | + | + | + | - | - | + | - | - | + | - | + | - | - | + | - | + | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | + | - | + | + | + | - | + | - | - | - | + | - | - | + | + | + | + | + | + | - | - | + | - |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
7047 | fresh water fish |
67770 | Freshwater fish |
119137 | Fresh water fish |
isolation source categories
- Cat1: #Host
- Cat2: #Fishes
taxonmaps
- @ref: 69479
- File name: preview.99_485.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_11;96_15;97_15;98_18;99_485&stattab=map
- Last taxonomy: Aeromonas
- 16S sequence: X60411
- Sequence Identity:
- Total samples: 313
- soil counts: 16
- aquatic counts: 156
- animal counts: 131
- plant counts: 10
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
7047 | yes, in single cases | yes, in single cases | 1 | Risk group (German classification) |
119137 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | A.eucrenophila (ATCC 23309T) gene for 16S ribosomal RNA | X74675 | 1460 | ena | 649 |
7047 | Aeromonas eucrenophila 16S rRNA gene, strain NCIMB 74 | X60411 | 1503 | ena | 649 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Aeromonas eucrenophila CECT 4224 | 649.3 | wgs | patric | 649 |
66792 | Aeromonas eucrenophila CECT 4224 | 2645727995 | draft | img | 649 |
67770 | Aeromonas eucrenophila CECT 4224 | GCA_000819865 | scaffold | ncbi | 649 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
gram-positive | no | 99.05 | no |
anaerobic | no | 97.913 | no |
halophile | no | 91.317 | no |
spore-forming | no | 96.395 | no |
glucose-util | yes | 91.699 | no |
thermophile | no | 99.591 | yes |
flagellated | yes | 89.834 | no |
aerobic | yes | 90.063 | no |
motile | yes | 90.196 | no |
glucose-ferment | yes | 89.244 | yes |
External links
@ref: 7047
culture collection no.: DSM 17534, ATCC 23309, CCEB 704, CCTM 885, CDC RH63, CIP 76.17, LMG 3774, NCIMB 74, JCM 8238, BCRC 13017, BCRC 14134, CCM 4354, CCUG 25942, CCUG 30340, CECT 4224, CNCTC 5356
straininfo link
- @ref: 69947
- straininfo: 9565
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 9336923 | Inclusion of Aeromonas DNA hybridization group 11 in Aeromonas encheleia and extended descriptions of the species Aeromonas eucrenophila and A. encheleia. | Huys G, Kampfer P, Altwegg M, Coopman R, Janssen P, Gillis M, Kersters K | Int J Syst Bacteriol | 10.1099/00207713-47-4-1157 | 1997 | Aeromonas/*classification/drug effects/genetics/physiology, Base Composition, Culture Media/metabolism, DNA, Bacterial/*analysis, Microbial Sensitivity Tests, Nucleic Acid Hybridization, Phenotype, *Phylogeny | Phenotype |
Phylogeny | 31935185 | Conservation of the name Aeromonas eucrenophila over the name Aeromonas punctata for the organism based on type stain NCMB 74(T) and universally defined as 'Aeromonas DNA hybridization group 6'. | Farmer JJ, Holmes B | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003961 | 2020 | Aeromonas/*classification, DNA, Bacterial/genetics, Nucleic Acid Hybridization, *Phylogeny |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
7047 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17534) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17534 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
37744 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/10786 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68368 | Automatically annotated from API 20E | |||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
69947 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID9565.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119137 | Curators of the CIP | Collection of Institut Pasteur (CIP 76.17) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2076.17 |