Strain identifier
BacDive ID: 2646
Type strain:
Species: Anaerotignum propionicum
Strain Designation: X2
Strain history: ATCC 25522 <-- L. DS. Smith VPI 5303 <-- M. Poston <-- E. Stadtman <-- H. Barker X2.
NCBI tax ID(s): 991789 (strain), 28446 (species)
General
@ref: 856
BacDive-ID: 2646
DSM-Number: 1682
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, chemoorganotroph, spore-forming, mesophilic, Gram-positive, motile, rod-shaped
description: Anaerotignum propionicum X2 is an anaerobe, chemoorganotroph, spore-forming bacterium that was isolated from black mud.
NCBI tax id
NCBI tax id | Matching level |
---|---|
991789 | strain |
28446 | species |
strain history
@ref | history |
---|---|
856 | <- ATCC <- L. DS. Smith; VPI 5303 <- J. M. Poston, National Institute of Health, Bethesda, Md., USA <- E. R. Stadtman <- H. A. Barker, University of California, Berkeley, USA; X2 {1942} |
67770 | ATCC 25522 <-- L. DS. Smith VPI 5303 <-- M. Poston <-- E. Stadtman <-- H. Barker X2. |
doi: 10.13145/bacdive2646.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Lachnospiraceae
- genus: Anaerotignum
- species: Anaerotignum propionicum
- full scientific name: Anaerotignum propionicum (Cardon and Barker 1946) Ueki et al. 2017
synonyms
@ref synonym 20215 Clostridium propionicum 20215 Tyzzerella propionica
@ref: 856
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Lachnospiraceae
genus: Anaerotignum
species: Anaerotignum propionicum
full scientific name: Anaerotignum propionicum (Cardon and Barker 1946) Ueki et al. 2017
strain designation: X2
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
43524 | positive | 1.3-5 µm | 0.5-0.8 µm | rod-shaped | yes | |
69480 | positive | 100 |
Culture and growth conditions
culture medium
- @ref: 856
- name: ANAEROTIGNUM MEDIUM (DSMZ Medium 156)
- growth: yes
- link: https://mediadive.dsmz.de/medium/156
- composition: Name: ANAEROTIGNUM MEDIUM (DSMZ Medium 156) Composition: Yeast extract 4.0 g/l L-Alanine 3.0 g/l Peptone 3.0 g/l NaHCO3 1.0 g/l L-Cysteine HCl x H2O 0.3 g/l MgSO4 x 7 H2O 0.1 g/l FeSO4 x 7 H2O 0.018 g/l Sodium resazurin 0.0005 g/l Potassium phosphate buffer CaSO4 Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
856 | positive | growth | 35 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
856 | anaerobe | |
43524 | obligate anaerobe | |
69480 | anaerobe | 99.999 |
nutrition type
- @ref: 43524
- type: chemoorganotroph
spore formation
@ref | spore formation | confidence |
---|---|---|
43524 | yes | |
69481 | yes | 100 |
compound production
- @ref: 856
- compound: acrylic acid
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43524 | 17234 | glucose | - | assimilation |
43524 | 18222 | xylose | - | assimilation |
43524 | 16977 | L-alanine | + | assimilation |
43524 | 17191 | L-isoleucine | + | assimilation |
43524 | 15603 | L-leucine | + | assimilation |
43524 | 17115 | L-serine | + | assimilation |
43524 | 16857 | L-threonine | + | assimilation |
43524 | 16414 | L-valine | + | assimilation |
43524 | 16236 | ethanol | +/- | assimilation |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
43524 | 30089 | acetate | yes |
43524 | 17968 | butyrate | yes |
43524 | 48944 | isobutyrate | yes |
43524 | 48942 | isovalerate | yes |
43524 | 17272 | propionate | yes |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
856 | black mud | California, San Francisco Bay | USA | USA | North America |
43524 | black mud | ||||
45180 | Mud | ||||
67770 | Black mud from San Francisco Bay | CA | USA | USA | North America |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Mud (Sludge)
taxonmaps
- @ref: 69479
- File name: preview.99_4084.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_41;96_2071;97_2496;98_3089;99_4084&stattab=map
- Last taxonomy: Anaerotignum
- 16S sequence: AB649276
- Sequence Identity:
- Total samples: 3179
- soil counts: 79
- aquatic counts: 241
- animal counts: 2798
- plant counts: 61
Safety information
risk assessment
- @ref: 856
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
856 | Clostridium propionicum 16S rRNA gene, strain DSM 1682 | X77841 | 1527 | ena | 991789 |
67770 | Clostridium propionicum gene for 16S ribosomal RNA, partial sequence, strain: JCM 1430 | AB649276 | 1495 | ena | 28446 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Anaerotignum propionicum DSM 1682 X2 | GCA_001561955 | complete | ncbi | 991789 |
66792 | Anaerotignum propionicum DSM 1682 | GCA_900129165 | scaffold | ncbi | 991789 |
66792 | [Clostridium] propionicum DSM 1682 | 991789.4 | wgs | patric | 991789 |
66792 | Anaerotignum propionicum DSM 1682 strain X2 | 991789.25 | complete | patric | 991789 |
66792 | Anaerotignum propionicum X2, DSM 1682 | 2582581271 | draft | img | 991789 |
66792 | Anaerotignum propionicum X2, DSM 1682 | 2808606817 | complete | img | 991789 |
GC content
- @ref: 43524
- GC-content: 36
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
flagellated | no | 64.223 | no |
gram-positive | yes | 81.261 | yes |
anaerobic | yes | 99.074 | yes |
aerobic | no | 98.56 | yes |
halophile | no | 79.875 | no |
spore-forming | yes | 80.705 | yes |
thermophile | no | 95.253 | no |
glucose-util | yes | 71.225 | yes |
motile | yes | 90.006 | yes |
glucose-ferment | yes | 53.899 | no |
External links
@ref: 856
culture collection no.: DSM 1682, ATCC 25522, NCIMB 10656, CCUG 9280, JCM 1430, VPI 5303
straininfo link
- @ref: 72177
- straininfo: 38577
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 1953298 | Isolation of Clostridium propionicum strain 19acry3 and further characteristics of the species. | Janssen PH | Arch Microbiol | 10.1007/BF00245351 | 1991 | Acrylates/*metabolism, Anaerobiosis, Biodegradation, Environmental, Clostridium/growth & development/*isolation & purification/metabolism/ultrastructure, Culture Media, Fermentation, Microscopy, Electron, Microscopy, Fluorescence, Microscopy, Phase-Contrast | Metabolism |
Genetics | 27081148 | Complete Genome Sequence of the Amino Acid-Fermenting Clostridium propionicum X2 (DSM 1682). | Poehlein A, Schlien K, Chowdhury NP, Gottschalk G, Buckel W, Daniel R | Genome Announc | 10.1128/genomeA.00294-16 | 2016 | ||
Phylogeny | 28905695 | Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum. | Ueki A, Goto K, Ohtaki Y, Kaku N, Ueki K | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002268 | 2017 | Animals, Bacterial Typing Techniques, Base Composition, Bioreactors/microbiology, Cattle, Clostridiales/*classification/genetics/isolation & purification, Clostridium/*classification, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Japan, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Waste Disposal, Fluid | Transcriptome |
Phylogeny | 31680219 | Anaerotignum faecicola sp. nov., isolated from human faeces. | Choi SH, Kim JS, Park JE, Lee KC, Eom MK, Oh BS, Yu SY, Kang SW, Han KI, Suh MK, Lee DH, Yoon H, Kim BY, Lee JH, Lee JH, Lee JS, Park SH | J Microbiol | 10.1007/s12275-019-9268-3 | 2019 | Bacterial Typing Techniques, Base Composition, Clostridiales/*classification/genetics/*isolation & purification/physiology, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Feces/*microbiology, Humans, Hydrogen-Ion Concentration, *Microbiota, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seoul, Sequence Analysis, DNA, Temperature | Pathogenicity |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
856 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1682) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1682 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
43524 | Atsuko Ueki, Kazushi Goto, Yoshimi Ohtaki, Nobuo Kaku, Katsuji Ueki | 10.1099/ijsem.0.002268 | Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum | IJSEM 67: 4146-4153 2017 | 28905695 | |
45180 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 9280) | https://www.ccug.se/strain?id=9280 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
72177 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID38577.1 | StrainInfo: A central database for resolving microbial strain identifiers |