Strain identifier

BacDive ID: 2645

Type strain: Yes

Species: Clostridium polysaccharolyticum

Strain Designation: B

Strain history: <- ATCC <- N. O. van Gylswyk, National Chemical Research Laboratory, Council for Scientific and Industrial Research, Pretoria, South Africa; B (Fusobacterium polysaccharolyticum)

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General

@ref: 660

BacDive-ID: 2645

DSM-Number: 1801

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive

description: Clostridium polysaccharolyticum B is an anaerobe, spore-forming, mesophilic bacterium that was isolated from sheep rumen.

NCBI tax id

NCBI tax idMatching level
1121329strain
29364species

strain history

  • @ref: 660
  • history: <- ATCC <- N. O. van Gylswyk, National Chemical Research Laboratory, Council for Scientific and Industrial Research, Pretoria, South Africa; B (Fusobacterium polysaccharolyticum)

doi: 10.13145/bacdive2645.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium polysaccharolyticum
  • full scientific name: Clostridium polysaccharolyticum (van Gylswyk 1981) van Gylswyk et al. 1983
  • synonyms

    • @ref: 20215
    • synonym: Fusobacterium polysaccharolyticum

@ref: 660

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium polysaccharolyticum

full scientific name: Clostridium polysaccharolyticum (van Gylswyk 1981) van Gylswyk et al. 1983

strain designation: B

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

Culture and growth conditions

culture medium

  • @ref: 660
  • name: RUMINICLOSTRIDIUM MEDIUM (DSMZ Medium 140)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/140
  • composition: Name: RUMINICLOSTRIDIUM MEDIUM (DSMZ Medium 140) Composition: Cellobiose 5.0 g/l Na2CO3 2.5 g/l Yeast extract 1.2 g/l NaCl 1.0 g/l K2HPO4 0.5 g/l (NH4)2SO4 0.5 g/l KH2PO4 0.5 g/l L-Cysteine HCl x H2O 0.25 g/l Na2S x 9 H2O 0.25 g/l CaCl2 x 2 H2O 0.1 g/l MgSO4 x 7 H2O 0.1 g/l Sodium resazurin 0.0005 g/l Sludge Rumen fluid Distilled water

culture temp

  • @ref: 660
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
660anaerobe
69480anaerobe99.434

spore formation

  • @ref: 69481
  • spore formation: yes
  • confidence: 92

Isolation, sampling and environmental information

isolation

  • @ref: 660
  • sample type: sheep rumen
  • geographic location: Pretoria
  • country: South Africa
  • origin.country: ZAF
  • continent: Africa

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Caprinae (Sheep/Goat)
#Host Body-Site#Organ#Rumen

Safety information

risk assessment

  • @ref: 660
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218C.polysaccharolyticum gene for 16S ribosomal RNAX718581523ena29364
660Clostridium polysaccharolyticum 16S rRNA gene, strain DSM 1801X778391509ena29364

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792[Clostridium] polysaccharolyticum DSM 1801GCA_900111595scaffoldncbi29364
66792[Clostridium] polysaccharolyticum strain DSM 180129364.8wgspatric29364
66792Lachnoclostridium polysaccharolyticum DSM 18012606217760draftimg29364

GC content

  • @ref: 660
  • GC-content: 41.6

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes92no
motileyes75.681no
flagellatedno54.855no
gram-positiveyes80.022no
anaerobicyes99.186yes
aerobicno97.106no
halophileno91.931no
spore-formingyes85.522no
glucose-fermentyes70.828no
thermophileno72.485no
glucose-utilyes87.73no

External links

@ref: 660

culture collection no.: DSM 1801, ATCC 33142

straininfo link

  • @ref: 72176
  • straininfo: 40354

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
660Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1801)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1801
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72176Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40354.1StrainInfo: A central database for resolving microbial strain identifiers