Strain identifier

BacDive ID: 2643

Type strain: No

Species: Clostridium perfringens

Strain Designation: ES-MS 22

Strain history: <- European Space Agency, Noordwijk, Netherlands; ES-MS 22 <- C. Moissl-Eichinger, Univ. Regensburg, Germany

NCBI tax ID(s): 1502 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 17129

BacDive-ID: 2643

DSM-Number: 30770

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, human pathogen

description: Clostridium perfringens ES-MS 22 is an anaerobe, spore-forming, mesophilic human pathogen that was isolated from spacecraft-associated clean room class ISO 8, Herschel Space Observatory.

NCBI tax id

  • NCBI tax id: 1502
  • Matching level: species

strain history

  • @ref: 17129
  • history: <- European Space Agency, Noordwijk, Netherlands; ES-MS 22 <- C. Moissl-Eichinger, Univ. Regensburg, Germany

doi: 10.13145/bacdive2643.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium perfringens
  • full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus perfringens

@ref: 17129

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium perfringens

full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937

strain designation: ES-MS 22

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

multimedia

  • @ref: 17129
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_30770.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

  • @ref: 17129
  • name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/110
  • composition: Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water

culture temp

  • @ref: 17129
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
17129anaerobe
69480anaerobe98.549

spore formation

@refspore formationconfidence
69481yes100
69480yes99.628

Isolation, sampling and environmental information

isolation

  • @ref: 17129
  • sample type: spacecraft-associated clean room class ISO 8, Herschel Space Observatory
  • geographic location: Noordwijk, European Space Research and Technology Centre
  • country: Netherlands
  • origin.country: NLD
  • continent: Europe

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Built environment
  • Cat3: #Clean room

taxonmaps

  • @ref: 69479
  • File name: preview.99_335.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_235;97_257;98_286;99_335&stattab=map
  • Last taxonomy: Clostridium perfringens subclade
  • 16S sequence: EU888515
  • Sequence Identity:
  • Total samples: 88326
  • soil counts: 3063
  • aquatic counts: 8762
  • animal counts: 75654
  • plant counts: 847

Safety information

risk assessment

  • @ref: 17129
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17129
  • description: Clostridium perfringens strain ES_MS22c 16S ribosomal RNA gene, partial sequence
  • accession: EU888515
  • length: 1275
  • database: ena
  • NCBI tax ID: 1502

Genome sequences

  • @ref: 66792
  • description: Clostridium perfringens strain FDAARGOS_932
  • accession: 1502.1790
  • assembly level: complete
  • database: patric
  • NCBI tax ID: 1502

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno81.919no
gram-positiveyes92.916no
anaerobicyes97.389yes
aerobicno96.341no
halophileno69.654no
spore-formingyes90.361no
glucose-utilyes90.4no
flagellatedno91.663no
thermophileno97.552yes
glucose-fermentyes77.203no

External links

@ref: 17129

culture collection no.: DSM 30770

straininfo link

  • @ref: 72174
  • straininfo: 401966

Reference

@idauthorscataloguedoi/urltitle
17129Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 30770)https://www.dsmz.de/collection/catalogue/details/culture/DSM-30770
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72174Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID401966.1StrainInfo: A central database for resolving microbial strain identifiers