Strain identifier

BacDive ID: 259

Type strain: Yes

Species: Aeromonas salmonicida subsp. pectinolytica

Strain Designation: 34mel, 34 mel

Strain history: CIP <- 2001, J. Zorzopulos, Buenos Aires Univ., Buenos Aires, Argentina: strain 34 mel

NCBI tax ID(s): 96473 (subspecies)

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General

@ref: 4770

BacDive-ID: 259

DSM-Number: 12609

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Aeromonas salmonicida subsp. pectinolytica 34mel is an aerobe, mesophilic, motile bacterium that was isolated from water.

NCBI tax id

  • NCBI tax id: 96473
  • Matching level: subspecies

strain history

@refhistory
4770<- M. E. Pavan; 34mel
396402001, J. Zorzopulos, Univ. Buenos Aires, Argentina: strain 34 mel
122395CIP <- 2001, J. Zorzopulos, Buenos Aires Univ., Buenos Aires, Argentina: strain 34 mel

doi: 10.13145/bacdive259.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Aeromonas
  • species: Aeromonas salmonicida subsp. pectinolytica
  • full scientific name: Aeromonas salmonicida subsp. pectinolytica Pavan et al. 2000

@ref: 4770

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Aeromonadales

family: Aeromonadaceae

genus: Aeromonas

species: Aeromonas salmonicida subsp. pectinolytica

full scientific name: Aeromonas salmonicida subsp. pectinolytica Pavan et al. 2000

strain designation: 34mel, 34 mel

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.558
6948099.994negative
122395nonegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4770NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39640MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122395CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
4770positivegrowth30mesophilic
39640positivegrowth30mesophilic
56550positivegrowth30-37mesophilic
122395positivegrowth5-37
122395nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
56550aerobe
122395facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.948

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin-builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68368L-arabinose+fermentation30849
68368amygdalin-fermentation27613
68368melibiose-fermentation28053
68368sucrose+fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol+fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol+fermentation16899
68368D-glucose+fermentation17634
68368gelatin+hydrolysis5291
68368tryptophan+energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368arginine+hydrolysis29016
122395mannitol-fermentation29864
122395citrate+carbon source16947
122395glucose+fermentation17234
122395lactose+fermentation17716
122395nitrate+reduction17632
122395nitrite-reduction16301
122395malonate-assimilation15792
122395sodium thiosulfate-builds gas from132112
122395glucose+degradation17234

antibiotic resistance

  • @ref: 122395
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleyes
6836816136hydrogen sulfideno
12239535581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole+
12239515688acetoin-
12239517234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase+1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
122395oxidase+
122395beta-galactosidase+3.2.1.23
122395alcohol dehydrogenase+1.1.1.1
122395gelatinase+
122395amylase+
122395DNase+
122395caseinase+3.4.21.50
122395catalase+1.11.1.6
122395tween esterase+
122395lecithinase+
122395lipase+
122395lysine decarboxylase-4.1.1.18
122395ornithine decarboxylase-4.1.1.17
122395phenylalanine ammonia-lyase-4.3.1.24
122395protease+
122395tryptophan deaminase-
122395urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122395-++++++-+-++-+-+++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
4770+++/------+++++-+-+--++

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122395+--++----++++----++-++-++-+++-++---++---------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122395+++++--+-+++++-+-++++-+--------+-+--+------------+---------++------------------++-+---+--++-+++----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
4770waterBuenos Aires, River MatanzaArgentinaARGMiddle and South America
56550WaterBuenos Aires,River MatanzaArgentinaARGMiddle and South America
122395Environment, Water, riverBuenos AiresArgentinaARGMiddle and South America1988

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_485.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_11;96_15;97_15;98_18;99_485&stattab=map
  • Last taxonomy: Aeromonas
  • 16S sequence: AF134065
  • Sequence Identity:
  • Total samples: 313
  • soil counts: 16
  • aquatic counts: 156
  • animal counts: 131
  • plant counts: 10

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
47701Risk group (German classification)
1223951Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Aeromonas salmonicida subsp. pectinolytica 16S ribosomal RNA gene, partial sequence
  • accession: AF134065
  • length: 1431
  • database: ena
  • NCBI tax ID: 96473

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aeromonas salmonicida subsp. pectinolytica 34melGCA_002735225completencbi1324960
66792Aeromonas salmonicida subsp. pectinolytica 34melGCA_000447435contigncbi1324960
66792Aeromonas salmonicida subsp. pectinolytica 34mel1324960.3wgspatric1324960
66792Aeromonas salmonicida subsp. pectinolytica 34mel1324960.19completepatric1324960

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedyes90.511no
gram-positiveno98.667no
anaerobicno97.497no
aerobicyes89.362yes
halophileno86.435no
spore-formingno94.614no
glucose-utilyes88.021no
thermophileno99.588yes
motileyes88.149no
glucose-fermentyes87.217yes

External links

@ref: 4770

culture collection no.: DSM 12609, CAIM 16, CECT 5752, CCUG 44929, CIP 107036

straininfo link

  • @ref: 69941
  • straininfo: 13238

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10843053Aeromonas salmonicida subsp. pectinolytica subsp. nov., a new pectinase-positive subspecies isolated from a heavily polluted river.Pavan ME, Abbott SL, Zorzopulos J, Janda JMInt J Syst Evol Microbiol10.1099/00207713-50-3-11192000Aeromonas/*classification/*enzymology/isolation & purification, DNA, Bacterial/genetics, Fresh Water/*microbiology, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Polygalacturonase/*metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Water Pollution, ChemicalEnzymology
Genetics24029754Genome Sequence of the Melanin-Producing Extremophile Aeromonas salmonicida subsp. pectinolytica Strain 34melT.Pavan ME, Pavan EE, Lopez NI, Levin L, Pettinari MJGenome Announc10.1128/genomeA.00675-132013
Genetics26025898Living in an Extremely Polluted Environment: Clues from the Genome of Melanin-Producing Aeromonas salmonicida subsp. pectinolytica 34melT.Pavan ME, Pavan EE, Lopez NI, Levin L, Pettinari MJAppl Environ Microbiol10.1128/AEM.00903-152015Adaptation, Biological, Aeromonas salmonicida/*genetics/isolation & purification/metabolism, Biotransformation, DNA, Bacterial/*chemistry/*genetics, Drug Resistance, Bacterial, Gene Transfer, Horizontal, *Genome, Bacterial, Melanins/metabolism, Metabolic Networks and Pathways/*genetics, Metals, Heavy/toxicity, Molecular Sequence Data, Pectins/metabolism, Rivers/microbiology, *Sequence Analysis, DNA, Water Pollution, ChemicalMetabolism
Genetics29304740The complete and fully assembled genome sequence of Aeromonas salmonicida subsp. pectinolytica and its comparative analysis with other Aeromonas species: investigation of the mobilome in environmental and pathogenic strains.Pfeiffer F, Zamora-Lagos MA, Blettinger M, Yeroslaviz A, Dahl A, Gruber S, Habermann BHBMC Genomics10.1186/s12864-017-4301-62018Aeromonas/genetics, Aeromonas salmonicida/*genetics/isolation & purification/pathogenicity, DNA Transposable Elements, Environmental Microbiology, Genes, Bacterial, *Genome, Bacterial, Genomics, High-Throughput Nucleotide Sequencing, *Interspersed Repetitive SequencesEnzymology
Metabolism30539256Glycerol inhibition of melanin biosynthesis in the environmental Aeromonas salmonicida 34mel(T).Pavan ME, Venero ES, Egoburo DE, Pavan EE, Lopez NI, Julia Pettinari MAppl Microbiol Biotechnol10.1007/s00253-018-9545-92018Aeromonas salmonicida/*metabolism, Amino Acids, Aromatic/metabolism, Biotransformation, Carbon/metabolism, Culture Media/chemistry, Gene Expression Regulation, Bacterial/drug effects, Glycerol/*metabolism, Melanins/*antagonists & inhibitorsCultivation

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4770Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12609)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12609
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39640Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19232
56550Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 44929)https://www.ccug.se/strain?id=44929
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69941Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13238.1StrainInfo: A central database for resolving microbial strain identifiers
122395Curators of the CIPCollection of Institut Pasteur (CIP 107036)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107036