Strain identifier

BacDive ID: 258

Type strain: Yes

Species: Aeromonas encheleia

Strain Designation: S181, S-181

Strain history: CIP <- 1992, C. Esteve, Valencia Univ., Spain: strain S-181

NCBI tax ID(s): 73010 (species)

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General

@ref: 4328

BacDive-ID: 258

DSM-Number: 11577

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, chemoorganotroph, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Aeromonas encheleia S181 is a facultative anaerobe, chemoorganotroph, mesophilic human pathogen that was isolated from healthy eel in fresh water.

NCBI tax id

  • NCBI tax id: 73010
  • Matching level: species

strain history

@refhistory
4328<- CECT <- C. Esteve; S181
119022CIP <- 1992, C. Esteve, Valencia Univ., Spain: strain S-181

doi: 10.13145/bacdive258.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Aeromonas
  • species: Aeromonas encheleia
  • full scientific name: Aeromonas encheleia Esteve et al. 1995

@ref: 4328

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Aeromonadales

family: Aeromonadaceae

genus: Aeromonas

species: Aeromonas encheleia

full scientific name: Aeromonas encheleia Esteve et al. 1995 emend. Huys et al. 1997

strain designation: S181, S-181

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
23255negativerod-shapedyes
119022negativerod-shapedyes

colony morphology

  • @ref: 23255
  • type of hemolysis: alpha/beta
  • hemolysis ability: 1
  • colony color: light brown
  • incubation period: 10-15 days
  • medium used: TSA

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4328CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
23255MacConkey agaryes
23255Trypticase Soy Agar (TSA)yes
41242MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
119022CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
4328positivegrowth28mesophilic
23255positivegrowth4psychrophilic
23255positivegrowth37mesophilic
41242positivegrowth30mesophilic
119022positivegrowth30-37mesophilic
119022nogrowth41thermophilic

culture pH

  • @ref: 23255
  • ability: positive
  • type: growth
  • pH: 9.0
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 119022
  • oxygen tolerance: facultative anaerobe

nutrition type

  • @ref: 23255
  • type: chemoorganotroph

halophily

  • @ref: 23255
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 3 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2325517057cellobiose-builds acid from
2325528053melibiose-builds acid from
2325516634raffinose-builds acid from
2325517924D-sorbitol-builds acid from
2325565327D-xylose-builds acid from
2325516813galactitol-builds acid from
2325517113erythritol-builds acid from
2325517754glycerol-builds acid from
2325530849L-arabinose-builds acid from
2325517716lactose-builds acid from
2325517268myo-inositol-builds acid from
23255309162-oxoglutarate-carbon source
2325516947citrate-carbon source
2325517057cellobiose-carbon source
232558391D-gluconate-carbon source
2325515748D-glucuronate-carbon source
2325516634raffinose-carbon source
23255370543-hydroxybutyrate-carbon source
2325516813galactitol-carbon source
2325517113erythritol-carbon source
2325516236ethanol-carbon source
2325515428glycine-carbon source
2325516977L-alanine-carbon source
2325530849L-arabinose-carbon source
2325516467L-arginine-carbon source
2325529991L-aspartate-carbon source
2325516349L-citrulline-carbon source
2325518050L-glutamine-carbon source
2325515603L-leucine-carbon source
2325562345L-rhamnose-carbon source
2325517895L-tyrosine-carbon source
2325517716lactose-carbon source
2325517268myo-inositol-carbon source
2325517272propionate-carbon source
2325517148putrescine-carbon source
2325512936D-galactose-growth
2325529985L-glutamate-growth
2325537397chondroitin sulfate-hydrolysis
232554767elastin-hydrolysis
2325516199urea-hydrolysis
2325512936D-galactose+builds acid from
2325516899D-mannitol+builds acid from
2325516024D-mannose+builds acid from
2325516551D-trehalose+builds acid from
2325517234glucose+builds acid from
2325562345L-rhamnose+builds acid from
2325517306maltose+builds acid from
2325517814salicin+builds acid from
2325517992sucrose+builds acid from
2325517234glucose+builds gas from
2325516899D-mannitol+carbon source
2325516024D-mannose+carbon source
2325516551D-trehalose+carbon source
2325529806fumarate+carbon source
2325515589L-malate+carbon source
2325517203L-proline+carbon source
2325517115L-serine+carbon source
2325517306maltose+carbon source
2325517814salicin+carbon source
2325517992sucrose+carbon source
2325516865gamma-aminobutyric acid+growth
2325517754glycerol+growth
2325515971L-histidine+growth
2325530031succinate+growth
2325518305arbutin+hydrolysis
23255casein+hydrolysis
2325517029chitin+hydrolysis
232553815collagen+hydrolysis
2325516991dna+hydrolysis
232554853esculin+hydrolysis
232555291gelatin+hydrolysis
2325528017starch+hydrolysis
2325553426tween 80+hydrolysis
2325517632nitrate+reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan+energy source
6836917632nitrate+reduction
6836830849L-arabinose-fermentation
6836827613amygdalin+fermentation
6836828053melibiose-fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin+hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis
11902216947citrate-carbon source
1190224853esculin+hydrolysis
11902217234glucose+fermentation
11902217716lactose-fermentation
11902217632nitrate+reduction
11902216301nitrite+reduction
119022132112sodium thiosulfate-builds gas from
11902217632nitrate+respiration

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
232552676amoxicillinyesyes
2325528971ampicillinyesyes
232553393carbenicillinyesyes
2325528915fosfomycinyesyes
2325528368novobiocinyesyes
2325527446phenoxymethylpenicillinyesyes
2325532161sulfadimethoxineyesyes
2325545373sulfanilamideyesyes
2325545924trimethoprimyesyes
2325573908vibriostatyesno
232552637amikacinyesyes
2325517698chloramphenicolyesyes
2325548923erythromycinyesyes
2325517833gentamicinyesyes
232556104kanamycinyesyes
23255100147nalidixic acidyesyes
2325571415nitrofurantoinyesyes
2325559066oxolinateyesyes
232558309polymyxin byesyes
2325528077rifampicinyesyes
2325517076streptomycinyesyes
2325527902tetracyclineyesyes
2325528864tobramycinyesyes
1190220129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)yesyesno

metabolite production

@refChebi-IDmetaboliteproduction
2325516136hydrogen sulfideno
2325515688acetoinno
2325535581indoleyes
6836935581indoleyes
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno
11902235581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
2325515688acetoin-
6836935581indole+
6836815688acetoin-
6836835581indole+
11902215688acetoin-

enzymes

@refvalueactivityec
23255arginine dihydrolase+3.5.3.6
23255catalase+1.11.1.6
23255cytochrome oxidase+1.9.3.1
23255lysine decarboxylase-4.1.1.18
23255ornithine decarboxylase-4.1.1.17
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
68368cytochrome oxidase+1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
119022oxidase+
119022beta-galactosidase+3.2.1.23
119022alcohol dehydrogenase+1.1.1.1
119022gelatinase-
119022amylase+
119022DNase+
119022caseinase+3.4.21.50
119022catalase+1.11.1.6
119022tween esterase+
119022gamma-glutamyltransferase+2.3.2.2
119022lecithinase+
119022lysine decarboxylase+4.1.1.18
119022ornithine decarboxylase-4.1.1.17
119022urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119022-+++++++--++----++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
4328++------+-+++--++-+-+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
4328++++-++++-++++++-+--+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119022+/----+----++++-+--+---+-++--+--++---++--+------+/---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119022+++++--+-++------+++---+-------+-+---------------+---------++------------------++-+------++++++--+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
4328healthy eel in fresh waterValenciaSpainESPEurope
119022Healthy European eels, Anguilla anguillaValenciaSpainESPEurope1987

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Host#Fishes

taxonmaps

  • @ref: 69479
  • File name: preview.99_485.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_11;96_15;97_15;98_18;99_485&stattab=map
  • Last taxonomy: Aeromonas
  • 16S sequence: HQ832414
  • Sequence Identity:
  • Total samples: 313
  • soil counts: 16
  • aquatic counts: 156
  • animal counts: 131
  • plant counts: 10

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
4328yes, in single casesyes, in single cases1Risk group (German classification)
1190221Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Aeromonas encheleia 16S rRNA gene, strain A 1881AJ2243091407ena73010
20218Aeromonas encheleia strain CECT4342 16S ribosomal RNA gene, partial sequenceHQ8324141503ena73010

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aeromonas encheleia CECT 4342GCA_000819825scaffoldncbi73010
66792Aeromonas encheleia CECT 434273010.3wgspatric73010
66792Aeromonas encheleia CECT 43422639762835draftimg73010

GC content

@refGC-contentmethod
432860.0thermal denaturation, midpoint method (Tm)
2325560.0Thermal denaturation, fluorometry

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes89.554no
flagellatedyes89.262no
gram-positiveno99.065no
anaerobicno98.142no
aerobicyes91.908no
halophileno90.884no
spore-formingno96.357no
glucose-utilyes92.297no
thermophileno99.681yes
glucose-fermentyes88.557yes

External links

@ref: 4328

culture collection no.: DSM 11577, CECT 4342, LMG 16330, CIP 104608, ATCC 51929

straininfo link

  • @ref: 69940
  • straininfo: 9342

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8590673Aeromonas encheleia sp. nov., isolated from European eels.Esteve C, Gutierrez MC, Ventosa AInt J Syst Bacteriol10.1099/00207713-45-3-4621995Aeromonas/*classification/drug effects/genetics/*isolation & purification/metabolism/pathogenicity, Amino Acids/metabolism, Anguilla/*microbiology, Animals, Base Composition, Carbohydrate Metabolism, Cell Movement, DNA, Bacterial/chemistry, Drug Resistance, Microbial, Indoles/metabolism, Nucleic Acid Hybridization, Penicillins/pharmacology, Phenotype, Sequence Homology, Nucleic Acid, Spain, Sulfonamides/pharmacologyMetabolism
Phylogeny9336923Inclusion of Aeromonas DNA hybridization group 11 in Aeromonas encheleia and extended descriptions of the species Aeromonas eucrenophila and A. encheleia.Huys G, Kampfer P, Altwegg M, Coopman R, Janssen P, Gillis M, Kersters KInt J Syst Bacteriol10.1099/00207713-47-4-11571997Aeromonas/*classification/drug effects/genetics/physiology, Base Composition, Culture Media/metabolism, DNA, Bacterial/*analysis, Microbial Sensitivity Tests, Nucleic Acid Hybridization, Phenotype, *PhylogenyPhenotype

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4328Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11577)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11577
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23255Consuelo Esteve, M. Carmen Gutiérrez, Antonio Ventosa10.1099/00207713-45-3-462Aeromonas encheleia sp. nov., Isolated from European EelsIJSEM 45: 462-466 19958590673
41242Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16536
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69940Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID9342.1StrainInfo: A central database for resolving microbial strain identifiers
119022Curators of the CIPCollection of Institut Pasteur (CIP 104608)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104608