Strain identifier

BacDive ID: 257

Type strain: Yes

Species: Aeromonas allosaccharophila

Strain Designation: 289

Strain history: CIP <- 1995, ATCC <- CECT <- C. Esteve

NCBI tax ID(s): 656 (species)

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General

@ref: 4327

BacDive-ID: 257

DSM-Number: 11576

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Aeromonas allosaccharophila 289 is a mesophilic, Gram-negative, motile bacterium that was isolated from diseased elver .

NCBI tax id

  • NCBI tax id: 656
  • Matching level: species

strain history

@refhistory
4327<- CECT <- A. J. Martinez-Murcia
116258CIP <- 1995, ATCC <- CECT <- C. Esteve

doi: 10.13145/bacdive257.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Aeromonas
  • species: Aeromonas allosaccharophila
  • full scientific name: Aeromonas allosaccharophila Martinez-Murcia et al. 1992

@ref: 4327

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Aeromonadales

family: Aeromonadaceae

genus: Aeromonas

species: Aeromonas allosaccharophila

full scientific name: Aeromonas allosaccharophila Martinez-Murcia et al. 1992

strain designation: 289

type strain: yes

Morphology

cell morphology

  • @ref: 116258
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4327CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
4327VIBRIO NATRIEGENS MEDIUM (DSMZ Medium 115)yeshttps://mediadive.dsmz.de/medium/115Name: VIBRIO NATRIEGENS MEDIUM (DSMZ Medium 115) Composition: NaCl 15.0 g/l Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
41247MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116258CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
4327positivegrowth28mesophilic
41247positivegrowth30mesophilic
116258positivegrowth25-30mesophilic
116258nogrowth41thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
116258NaClpositivegrowth0-4 %
116258NaClnogrowth6 %
116258NaClnogrowth8 %
116258NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68369malate+assimilation25115
68369adipate-assimilation17128
68369decanoate+assimilation27689
68369gluconate+assimilation24265
68369maltose+assimilation17306
68369N-acetylglucosamine+assimilation59640
68369D-mannitol+assimilation16899
68369D-mannose+assimilation16024
68369L-arabinose+assimilation30849
68369D-glucose+assimilation17634
68369gelatin+hydrolysis5291
68369esculin+hydrolysis4853
68369urea-hydrolysis16199
68369arginine+hydrolysis29016
68369D-glucose+fermentation17634
68369tryptophan+energy source27897
68369nitrate+reduction17632
68368L-arabinose+fermentation30849
68368amygdalin+fermentation27613
68368melibiose-fermentation28053
68368sucrose+fermentation17992
68368L-rhamnose+fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol+fermentation16899
68368D-glucose+fermentation17634
68368gelatin+hydrolysis5291
68368tryptophan+energy source27897
68368urea-hydrolysis16199
68368citrate-assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine+hydrolysis29016
116258glucose+fermentation17234
116258lactose-fermentation17716
116258nitrate+reduction17632
116258nitrite-reduction16301
116258sodium thiosulfate-builds gas from132112

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleyes
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6836935581indole+
6836815688acetoin-
6836835581indole+
11625815688acetoin-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
68368cytochrome oxidase+1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
116258oxidase+
116258beta-galactosidase+3.2.1.23
116258alcohol dehydrogenase+1.1.1.1
116258gelatinase+
116258DNase+
116258caseinase+3.4.21.50
116258catalase+1.11.1.6
116258tween esterase+
116258lecithinase+
116258lipase+
116258lysine decarboxylase-4.1.1.18
116258ornithine decarboxylase-4.1.1.17
116258protease+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116258-+++++--+-+--+---+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
4327++------+-+++--++-+++

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
4327++++-+++++++++++-+--+
4327++++-+++++++++++-+--+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116258+--++----++++-+--+---+--+/--++--++---++----+-------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116258+++++--+-++--+-+-++++--+-------+-+---------------+---------++---------------+--++-----+--++++++--++

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentsampling date
4327diseased elver (Anguilla anguilla)Anguilla anguillaValenciaSpainESPEurope
57561Eal,diseased,Anguilla anguillaSpainESPEurope1988
116258Diseased elversValenciaSpainESPEurope

isolation source categories

  • Cat1: #Host
  • Cat2: #Fishes

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
43272Risk group (German classification)
1162581Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Aeromonas allosaccharophila strain CECT 4199 16S ribosomal RNA gene, partial sequenceJX014444151ena656
4327Aeromonas allosaccharophila strain CECT 4199 16S ribosomal RNA gene, partial sequenceS392321503ena656

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aeromonas allosaccharophila CECT 4199GCA_000819685scaffoldncbi656
66792Aeromonas allosaccharophila CECT 4199656.3wgspatric656
66792Aeromonas allosaccharophila CECT 41992648501309draftimg656

GC content

  • @ref: 4327
  • GC-content: 59.5
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes89.269no
flagellatedyes86.433no
gram-positiveno98.752no
anaerobicno95.023no
aerobicyes83.777no
halophileno88.151no
spore-formingno95.25no
glucose-utilyes91.748no
thermophileno99.391yes
glucose-fermentyes87.112yes

External links

@ref: 4327

culture collection no.: DSM 11576, ATCC 51208, CECT 4199, CCUG 47180 A, CIP 104616

straininfo link

  • @ref: 69939
  • straininfo: 92313

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1378035Aeromonas allosaccharophila sp. nov., a new mesophilic member of the genus Aeromonas.Martinez-Murcia AJ, Esteve C, Garay E, Collins MDFEMS Microbiol Lett10.1016/0378-1097(92)90698-n1992Aeromonas/*classification/genetics, Base Sequence, Enzymes/analysis, Molecular Sequence Data, Phylogeny, RNA, Bacterial/*chemistry, RNA, Ribosomal, 16S/*genetics, Sequence Homology, Nucleic Acid, Tetracycline ResistanceGenetics
Phylogeny7727276DNA relatedness among Aeromonas allosaccharophila strains and DNA hybridization groups of the genus Aeromonas.Esteve C, Gutierrez MC, Ventosa AInt J Syst Bacteriol10.1099/00207713-45-2-3901995Aeromonas/classification/*genetics, Animals, DNA, Bacterial/*analysis, Nucleic Acid Hybridization
Phylogeny11518320New DNA-DNA hybridization and phenotypic data on the species Aeromonas ichthiosmia and Aeromonas allosaccharophila: A. ichthiosmia Schubert et al. 1990 is a later synonym of A. veronii Hickman-Brenner et al. 1987.Huys G, Kampfer P, Swings JSyst Appl Microbiol10.1078/0723-2020-000382001Aeromonas/*classification/*genetics/metabolism/physiology, Bacterial Typing Techniques/*methods, DNA, Bacterial/*analysis, Nucleic Acid Hybridization, PhenotypePhenotype
Phylogeny17080292Phylogenetic identification of Aeromonas strains isolated from carcasses of pig as new members of the species Aeromonas allosaccharophila.Saavedra MJ, Perea V, Fontes MC, Martins C, Martinez-Murcia AAntonie Van Leeuwenhoek10.1007/s10482-006-9107-52007Abattoirs, Aeromonas/*classification/genetics/*isolation & purification, Animals, Bacterial Proteins/genetics, Bacterial Typing Techniques, DNA Fingerprinting, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Equipment and Supplies/microbiology, Food Microbiology, Gastroenteritis/microbiology, Genes, rRNA, Genotype, Gram-Negative Bacterial Infections/microbiology, Humans, Meat/*microbiology, Molecular Epidemiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Swine/*microbiologyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4327Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11576)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11576
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41247Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16544
57561Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47180 A)https://www.ccug.se/strain?id=47180
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69939Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92313.1StrainInfo: A central database for resolving microbial strain identifiers
116258Curators of the CIPCollection of Institut Pasteur (CIP 104616)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104616