Strain identifier

BacDive ID: 2529

Type strain: Yes

Species: Clostridium acetobutylicum

Strain Designation: 2291, W

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General

@ref: 449

BacDive-ID: 2529

DSM-Number: 792

keywords: mesophilic, anaerobe, Bacteria, 16S sequence, genome sequence, alcohol production

description: Clostridium acetobutylicum 2291 is an anaerobe, mesophilic bacterium that produces alcohol and was isolated from cornmeal.

strain history: <- ATCC <- E. R. Weyer, Yale University, Laboratory of General Bacteriology, New Haven, Connecticut, USA (Granulobacter pectinovorum)

doi: 10.13145/bacdive2529.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/firmicutes
  • domain: Bacteria
  • phylum: Firmicutes
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium acetobutylicum
  • full scientific name: Clostridium acetobutylicum McCoy et al. 1926 (Approved Lists 1980)

@ref: 449

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium acetobutylicum

full scientific name: Clostridium acetobutylicum McCoy et al. 1926 emend. Keis et al. 2001

strain designation: 2291, W

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlink
449CLOSTRIDIUM ACETOBUTYLICUM MEDIUM (DSMZ Medium 411), anaerobicyeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium411.pdf
449PY + X MEDIUM (DSMZ Medium 104b), anaerobicyeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf

culture temp

@refgrowthtypetemperaturerange
449positivegrowth37mesophilic
55354positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
449anaerobe
55354anaerobe

compound production

@refcompound
449acetone
449n butanol
449autobacteriocin
449acidolysin

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
55354--++------+----++---

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
55354----+++---+------------------

Isolation, sampling and environmental information

isolation

  • @ref: 449
  • sample type: cornmeal

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Engineered#Food production

Safety information

risk assessment

  • @ref: 449
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Clostridium acetobutylicum ATCC 824 16S ribosomal RNA (rrn) gene, partial sequenceU161661411ena272562
20218Clostridium acetobutylicum DSM 792 16S ribosomal RNA (rrn) gene, partial sequenceU17030554ena272562
20218C.acetobutylicum (ATCC 824, T) 16S rRNAX780701389ena272562

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridium acetobutylicum ATCC 824GCA_000008765completencbi272562
66792Clostridium acetobutylicum LJ4GCA_003254825chromosomencbi1488
66792Clostridium acetobutylicum DJ024GCA_013297095contigncbi1488
66792Clostridium acetobutylicum DJ310GCA_013296345contigncbi1488
66792Clostridium acetobutylicum NCCB 24020GCA_002006385contigncbi1488
66792Clostridium acetobutylicum BOH3GCA_006546765scaffoldncbi1488
66792Clostridium acetobutylicum WAGCA_002954145scaffoldncbi1488
66792Clostridium acetobutylicum JB200GCA_002216285scaffoldncbi1488
66792Clostridium acetobutylicum GXAS18-1GCA_000785855scaffoldncbi1488
66792Clostridium acetobutylicum ATCC 824272562.8completepatric272562
66792Clostridium acetobutylicum ATCC 824272562.61plasmidpatric272562
66792Clostridium acetobutylicum GXAS18-11488.5wgspatric1488
66792Clostridium acetobutylicum strain BOH31488.48wgspatric1488
66792Clostridium acetobutylicum strain LJ41488.39completepatric1488
66792Clostridium acetobutylicum strain NCCB 240201488.19wgspatric1488
66792Clostridium acetobutylicum strain WA1488.38wgspatric1488
66792Clostridium acetobutylicum DJ313 (Re-Annotation)2853669153permanentimg1488
66792Clostridium acetobutylicum DJ311 (Re-Annotation)2875888368permanentimg1488
66792Clostridium acetobutylicum DJ0242706794910permanentimg1488
66792Clostridium acetobutylicum DJ310 (Re-Annotation)2853664947permanentimg1488
66792Clostridium acetobutylicum DJ024 (Re-Annotation)2852917358permanentimg1488
66792Clostridium acetobutylicum DJ0742724679640permanentimg1488
66792Clostridium acetobutylicum DJ3102772190829permanentimg1488
66792Clostridium acetobutylicum DJ074 (Re-Annotation)2853072447permanentimg1488
66792Clostridium acetobutylicum DJ3132772190827permanentimg1488
66792Clostridium acetobutylicum DJ3112772190980permanentimg1488

GC content

  • @ref: 449
  • GC-content: 28.0

External links

@ref: 449

culture collection no.: DSM 792, ATCC 824, VKM B-1787, CCUG 42182 C, LMG 5710, CECT 508, IFO 13948

straininfo link

@refpassport
20218http://www.straininfo.net/strains/7820
20218http://www.straininfo.net/strains/7818
20218http://www.straininfo.net/strains/156989

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
449Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 792)https://www.dsmz.de/collection/catalogue/details/culture/DSM-792
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
55354Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 42182 C)https://www.ccug.se/strain?id=42182
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)