Strain identifier
BacDive ID: 2516
Type strain: 
Species: Thermobrachium celere
Strain Designation: JW/YL-NZ35
Strain history: <- J. Wiegel; JW/YL-NZ35 <- Y. Li and J. Wiegel {1992}
NCBI tax ID(s): 941824 (strain), 53422 (species)
version 8.1 (current version)
General
@ref: 3344
BacDive-ID: 2516
DSM-Number: 8682
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, motile
description: Thermobrachium celere JW/YL-NZ35 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from hot spring sediment.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 53422 | species |
| 941824 | strain |
strain history
- @ref: 3344
- history: <- J. Wiegel; JW/YL-NZ35 <- Y. Li and J. Wiegel {1992}
doi: 10.13145/bacdive2516.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Thermobrachium
- species: Thermobrachium celere
- full scientific name: Thermobrachium celere Engle et al. 1996
synonyms
- @ref: 20215
- synonym: Caloramator celer
@ref: 3344
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Thermobrachium
species: Thermobrachium celere
full scientific name: Thermobrachium celere Engle et al. 1996
strain designation: JW/YL-NZ35
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 96.729 | |
| 69480 | 99.999 | positive |
Culture and growth conditions
culture medium
- @ref: 3344
- name: YTG MEDIUM (DSMZ Medium 616)
- growth: yes
- link: https://mediadive.dsmz.de/medium/616
- composition: Name: CLOSTRIDIUM (YTG) MEDIUM (DSMZ Medium 616) Composition: Tryptone 10.0 g/l Na2CO3 5.3 g/l Yeast extract 5.0 g/l D-Glucose 3.0 g/l Na2HPO4 x 2 H2O 0.36 g/l Na2S x 9 H2O 0.2 g/l L-Cysteine HCl x H2O 0.2 g/l KCl 0.08 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 3344 | positive | growth | 55 | thermophilic | |
| 69480 | thermophilic | 98.624 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 3344 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
Isolation, sampling and environmental information
isolation
- @ref: 3344
- sample type: hot spring sediment
- geographic location: Ohinimutu
- country: New Zealand
- origin.country: NZL
- continent: Australia and Oceania
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Sediment |
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_4938.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_285;96_2427;97_2956;98_3697;99_4938&stattab=map
- Last taxonomy: Thermobrachium celere
- 16S sequence: X99238
- Sequence Identity:
- Total samples: 323
- soil counts: 35
- aquatic counts: 159
- animal counts: 121
- plant counts: 8
Safety information
risk assessment
- @ref: 3344
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 3344
- description: T.celer 16S rRNA gene
- accession: X99238
- length: 1404
- database: ena
- NCBI tax ID: 941824
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermobrachium celere DSM 8682 | GCA_000430995 | scaffold | ncbi | 941824 |
| 66792 | Thermobrachium celere DSM 8682 | 941824.4 | wgs | patric | 941824 |
| 66792 | Thermobrachium celere JW/YL-NZ35, DSM 8682 | 2519899721 | draft | img | 941824 |
GC content
- @ref: 3344
- GC-content: 31
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | yes | 84.027 | no |
| flagellated | no | 65.802 | no |
| gram-positive | yes | 57.949 | no |
| anaerobic | yes | 99.416 | no |
| aerobic | no | 97.977 | yes |
| halophile | no | 87.436 | no |
| spore-forming | yes | 87.176 | no |
| glucose-util | yes | 89.803 | no |
| thermophile | yes | 99.713 | yes |
| glucose-ferment | yes | 63.985 | no |
External links
@ref: 3344
culture collection no.: DSM 8682
straininfo link
- @ref: 72051
- straininfo: 43877
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 8863432 | Thermobrachium celere gen. nov., sp. nov., a rapidly growing thermophilic, alkalitolerant, and proteolytic obligate anaerobe. | Engle M, Li Y, Rainey F, DeBlois S, Mai V, Reichert A, Mayer F, Messner P, Wiegel J | Int J Syst Bacteriol | 10.1099/00207713-46-4-1025 | 1996 | Bacteria, Anaerobic/chemistry/*classification/metabolism, Base Sequence, DNA, Ribosomal/chemistry, Fermentation, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics | Genetics |
| Genetics | 23868125 | Draft Genome Sequence of the Hydrogen- and Ethanol-Producing Anaerobic Alkalithermophilic Bacterium Caloramator celer. | Ciranna A, Larjo A, Kivisto A, Santala V, Roos C, Karp M | Genome Announc | 10.1128/genomeA.00471-13 | 2013 | ||
| Metabolism | 35157106 | Biological cellulose saccharification using a coculture of Clostridium thermocellum and Thermobrachium celere strain A9. | Nhim S, Waeonukul R, Uke A, Baramee S, Ratanakhanokchai K, Tachaapaikoon C, Pason P, Liu YJ, Kosugi A | Appl Microbiol Biotechnol | 10.1007/s00253-022-11818-0 | 2022 | Cellulose/metabolism, Clostridiaceae, *Clostridium thermocellum/genetics/metabolism, Coculture Techniques, RNA, Ribosomal, 16S/genetics/metabolism, beta-Glucosidase/metabolism | Phylogeny |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 3344 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8682) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-8682 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 72051 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID43877.1 | StrainInfo: A central database for resolving microbial strain identifiers |