Strain identifier

BacDive ID: 2511

Type strain: No

Species: unclassified bacterium

Strain Designation: LD64

Strain history: <- N. O. van Gylswyk, Swedish Univ. Agric. Sci., Uppsala, Sweden; LD64 (Sarcina sp.) <- N. O. van Gylswyk {1991}

NCBI tax ID(s): 1869227 (species)

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General

@ref: 4155

BacDive-ID: 2511

DSM-Number: 11001

keywords: genome sequence, 16S sequence, anaerobe, mesophilic, Gram-positive

description: unclassified bacterium LD64 is an anaerobe, mesophilic, Gram-positive prokaryote that was isolated from cow rumen, grass-silage diet.

NCBI tax id

  • NCBI tax id: 1869227
  • Matching level: species

strain history

  • @ref: 4155
  • history: <- N. O. van Gylswyk, Swedish Univ. Agric. Sci., Uppsala, Sweden; LD64 (Sarcina sp.) <- N. O. van Gylswyk {1991}

doi: 10.13145/bacdive2511.20230509.8.1

Name and taxonomic classification

@ref: 4155

phylum: Not assigned to order

class: Not assigned to order

order: Clostridiales

family: Not assigned to family

genus: unclassified

species: unclassified bacterium

full scientific name: unclassified bacterium

strain designation: LD64

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 99.719

Culture and growth conditions

culture medium

  • @ref: 4155
  • name: SUCCINICLASTICUM MEDIUM (DSMZ Medium 666)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/666
  • composition: Name: SUCCINICLASTICUM MEDIUM (DSMZ Medium 666; with strain-specific modifications) Composition: NaHCO3 6.4 g/l NaCl 0.45 g/l (NH4)2SO4 0.45 g/l Na2S x 9 H2O 0.3 g/l L-Cysteine HCl x H2O 0.3 g/l KH2PO4 0.23 g/l K2HPO4 0.23 g/l MgSO4 x 7 H2O 0.09 g/l CaCl2 x 2 H2O 0.06 g/l Indigocarmine 0.005 g/l Rumen fluid Distilled water

culture temp

  • @ref: 4155
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
4155anaerobe
69480anaerobe98.828

spore formation

@refspore formationconfidence
69481no100
69480no99.998

Isolation, sampling and environmental information

isolation

  • @ref: 4155
  • sample type: cow rumen, grass-silage diet
  • country: Sweden
  • origin.country: SWE
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Organ#Rumen

taxonmaps

  • @ref: 69479
  • File name: preview.99_42411.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_780;96_4829;97_8047;98_30817;99_42411&stattab=map
  • Last taxonomy: Lachnospiraceae
  • 16S sequence: MN336244
  • Sequence Identity:
  • Total samples: 6434
  • soil counts: 432
  • aquatic counts: 178
  • animal counts: 5606
  • plant counts: 218

Sequence information

16S sequences

  • @ref: 4155
  • description: Bacterium strain DSM _11001 16S ribosomal RNA gene, partial sequence
  • accession: MN336244
  • length: 1518
  • database: ena
  • NCBI tax ID: 1869227

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sarcina sp. DSM 11001GCA_900101015scaffoldncbi1798184
66792Sarcina sp. DSM 11001 Rumen_Coassembly_mag_1199GCA_905235985contigncbi1798184
66792Sarcina sp. DSM 110011798184.3wgspatric1798184
66792Sarcina sp. DSM 11001 strain Rumen_Coassembly_mag_11991798184.6wgspatric1798184
66792Sarcina sp. DSM 110012608642105draftimg1798184

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno90.478no
flagellatedno95.981no
gram-positiveyes82.581no
anaerobicyes99.242yes
aerobicno98.324yes
halophileno86.313no
spore-formingno69.138no
glucose-fermentyes74.969no
thermophileno96.259yes
glucose-utilyes82.654no

External links

@ref: 4155

culture collection no.: DSM 11001

Reference

@idauthorscataloguedoi/urltitle
4155Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11001)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11001
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1