Strain identifier

BacDive ID: 24815

Type strain: Yes

Species: Thermosipho activus

Strain Designation: Rift-s3

Strain history: <- O. A. Podosocorskaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; Rift-s3 <- O. A. Podosokorskaya {2010}

NCBI tax ID(s): 1462921 (species)

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General

@ref: 21063

BacDive-ID: 24815

DSM-Number: 26467

keywords: 16S sequence, Bacteria, anaerobe, thermophilic

description: Thermosipho activus Rift-s3 is an anaerobe, thermophilic bacterium that was isolated from Riftia pachyptila sheath scraping, 2004 m depth.

NCBI tax id

  • NCBI tax id: 1462921
  • Matching level: species

strain history

  • @ref: 21063
  • history: <- O. A. Podosocorskaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; Rift-s3 <- O. A. Podosokorskaya {2010}

doi: 10.13145/bacdive24815.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/thermotogota
  • domain: Bacteria
  • phylum: Thermotogota
  • class: Thermotogae
  • order: Thermotogales
  • family: Fervidobacteriaceae
  • genus: Thermosipho
  • species: Thermosipho activus
  • full scientific name: Thermosipho activus Podosokorskaya et al. 2014

@ref: 21063

domain: Bacteria

phylum: Thermotogae

class: Thermotogae

order: Fervidobacteriaceae, not assigned to order

family: Fervidobacteriaceae

genus: Thermosipho

species: Thermosipho activus

full scientific name: Thermosipho activus Podosokorskaya et al. 2014

strain designation: Rift-s3

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 21063
  • name: THERMOSIPHO MJ MEDIUM (DSMZ Medium 1011b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1011b
  • composition: Name: THERMOSIPHO MJ MEDIUM (DSMZ Medium 1011b) Composition: NaCl 19.9601 g/l MgSO4 x 7 H2O 3.44311 g/l MgCl2 x 6 H2O 2.74451 g/l D-Glucose 1.99601 g/l Na2CO3 0.998004 g/l KCl 0.329341 g/l Na2S x 9 H2O 0.299401 g/l L-Cysteine HCl x H2O 0.299401 g/l NH4Cl 0.249501 g/l K2HPO4 0.149701 g/l CaCl2 x 2 H2O 0.149701 g/l Yeast extract 0.0998004 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00998004 g/l Sodium resazurin 0.000499002 g/l H3BO3 0.000299401 g/l CoCl2 x 6 H2O 0.000199601 g/l Pyridoxine hydrochloride 9.98004e-05 g/l ZnSO4 x 7 H2O 9.98004e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Na2MoO4 x 2 H2O 2.99401e-05 g/l MnCl2 x 4 H2O 2.99401e-05 g/l Folic acid 1.99601e-05 g/l Biotin 1.99601e-05 g/l NiCl2 x 6 H2O 1.99601e-05 g/l CuCl2 x 2 H2O 9.98004e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

culture temp

  • @ref: 21063
  • growth: positive
  • type: growth
  • temperature: 65
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 21063
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 21063
  • sample type: Riftia pachyptila sheath scraping, 2004 m depth
  • host species: Riftia pachyptila
  • geographic location: Gulf of California, Guaymas Basin
  • country: Mexico
  • origin.country: MEX
  • continent: North America
  • latitude: 27.0065
  • longitude: -111.425

isolation source categories

  • Cat1: #Host
  • Cat2: #Invertebrates (Other)
  • Cat3: #Annelida

taxonmaps

  • @ref: 69479
  • File name: preview.99_156728.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_93;96_2192;97_2651;98_103299;99_156728&stattab=map
  • Last taxonomy: Thermosipho activus subclade
  • 16S sequence: KF931642
  • Sequence Identity:
  • Total samples: 24
  • aquatic counts: 24

Safety information

risk assessment

  • @ref: 21063
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21063
  • description: Thermosipho activus strain Rift-s3 16S ribosomal RNA gene, partial sequence
  • accession: KF931642
  • length: 1519
  • database: ena
  • NCBI tax ID: 1462921

GC content

  • @ref: 21063
  • GC-content: 30
  • method: sequence analysis

External links

@ref: 21063

culture collection no.: DSM 26467, VKM B-2803

straininfo link

  • @ref: 88636
  • straininfo: 405362

literature

  • topic: Phylogeny
  • Pubmed-ID: 24994778
  • title: Thermosipho activus sp. nov., a thermophilic, anaerobic, hydrolytic bacterium isolated from a deep-sea sample.
  • authors: Podosokorskaya OA, Bonch-Osmolovskaya EA, Godfroy A, Gavrilov SN, Beskorovaynaya DA, Sokolova TG, Kolganova TV, Toshchakov SV, Kublanov IV
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.063156-0
  • year: 2014
  • mesh: Bacterial Typing Techniques, Base Composition, California, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Gram-Negative Anaerobic Straight, Curved, and Helical Rods/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21063Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26467Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26467)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88636Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405362.1