General

@ref: 21012

BacDive-ID: 24809

DSM-Number: 27849

keywords: Archaea, anaerobe, mesophilic

description: Methanocalculus chunghsingensis 01F9704a is an anaerobe, mesophilic archaeon that was isolated from sediment sample of estuarine.

NCBI tax id

• NCBI tax id: 156457
• Matching level: species

strain history

• @ref: 21012
• history: <- M.-C. Lai, National Chung Hsing Univ., Dept. of Botany, Taiwan; 01F9704a <- M.-C. Lai {1997}

doi: 10.13145/bacdive24809.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanomicrobiales
• family: Methanocalculaceae
• genus: Methanocalculus
• species: Methanocalculus chunghsingensis
• full scientific name: Methanocalculus chunghsingensis Lai et al. 2004

@ref: 21012

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanocalculaceae

genus: Methanocalculus

species: Methanocalculus chunghsingensis

full scientific name: Methanocalculus chunghsingensis Lai et al. 2004

strain designation: 01F9704a

type strain: no

Morphology

colony morphology

• @ref: 21012
• incubation period: 2-3 days

Culture and growth conditions

culture medium

• @ref: 21012
• name: METHANOCALCULUS MB MEDIUM (DSMZ Medium 924)
• growth: yes
• link: https://mediadive.dsmz.de/medium/924
• composition: Name: METHANOCALCULUS MB MEDIUM (DSMZ Medium 924) Composition: Na-formate 6.72601 g/l NaHCO3 3.95648 g/l Trypticase peptone 1.97824 g/l Yeast extract 1.97824 g/l Na-acetate 1.58259 g/l MgCl2 x 6 H2O 0.98912 g/l NH4Cl 0.98912 g/l KCl 0.49456 g/l K2HPO4 0.395648 g/l Na2S x 9 H2O 0.24728 g/l L-Cysteine HCl x H2O 0.24728 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

• @ref: 21012
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 21012
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 21012
• sample type: sediment sample of estuarine
• geographic location: Taiwan, Eriln Shi
• country: China
• origin.country: CHN
• continent: Asia

isolation source categories

Safety information

risk assessment

• @ref: 21012
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

External links

@ref: 21012

culture collection no.: DSM 27849, OCM 747

straininfo link

• @ref: 88631
• straininfo: 397009

Reference